The activation mechanisms of mechanosensitive cation channels in boneremodeling.

• Project/Area Number: 20791349
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Tohoku University
• Principal Investigator: YOSHIDA Takashi Tohoku University, 大学院・歯学研究科, 助教 (30455795)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 骨芽細胞 / チャネル / RT-PCR法 / 機械的刺激 / カルシウム / real-time PCR法 / MC3T3-E1細胞 / 発現調節

Research Abstract

Mechanical stress plays a vital role in maintaining bone architecture. The process by which osteoblasts convert mechanical signal into biochemical responses leading to bone remodeling is not fully understood. In spite of the significant role of channels in osteoblast, the molecular identity of mechanosensitive channels remains unknown. To identify the mechanosensitive channels, we examined the expression level of channels in the clonal mouse osteoblast cell line MC3T3-E1. RT-PCR analysis revealed some mechanosensitive channel genes are expressed in MC3T3-E1 cells.

Research Products

• [Journal Article] Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound. (2009)
  • Author(s): Kiyonaka S, Kato K, Nishida M, Mio K, Numaga T, Sawaguchi Y, Yoshida T, Wakamori M, Mori E, Numata T, Ishii M, Takemoto H, Ojida A, Watanabe K, Uemura A, Kurose H, Morii T, Kobayashi T, Sato Y, Sato C, Hamachi I, Mori Y.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 106巻 Pages: 5400-5404
  • Peer Reviewed
• [Journal Article] Selective and direct inhibition of TRPC3 channels un derlies biological activities of a pyrazole compound (2009)
  • Author(s): Shigeki Kiyonaka et.al.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 106 Pages: 5400-5405
  • Peer Reviewed
• [Presentation] TRPM5チャネル活性の脂肪酸による修飾 (2010)
  • Author(s): 若森実、吉田卓史、菊地尚
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2010-03-17
• [Presentation] TRPC5チャネルの新規活性化機構と生理的意義 (2009)
  • Author(s): 吉田卓史、森泰生
  • Organizer: 第37回薬物活性シンポジウム
  • Place of Presentation: 東北薬科大学(仙台)
  • Year and Date: 2009-10-10
• [Presentation] MC3T3-E1細胞における機械刺激受容に関与するTRPチャネル (2009)
  • Author(s): 吉田卓史、宮島悠旗、若森実
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ(新潟)
  • Year and Date: 2009-09-11
• [Presentation] TRP CHANNELS PLAY A ROLE IN MECHANICAL RESPONSES IN STRETCHED OSTEOBLASTS. (2009)
  • Author(s): Takashi Yoshida, Yuki Miyajima, Minoru Wakamori.
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2009-07-30
• [Presentation] TRP CHANNELS PLAY A ROLE IN MECHANICAL RESPONSES IN STRETCHED OSTEOBLASTS (2009)
  • Author(s): 吉田卓史, 宮島悠旗, 若森実
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2009-07-30
• [Presentation] マウス骨芽細胞株MC3T3-E1におけるTRPメカノセンサーチャネルの発現解析 (2009)
  • Author(s): 吉田卓史、宮島悠旗、若森実
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-16
• [Presentation] マウス骨芽細胞株MC3T3-E1におけるTRPメカノセンサーチャネルの発現解析 (2009)
  • Author(s): 吉田卓史、宮島悠旗、若森実
  • Organizer: 日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-16
• [Presentation] Expression patterns of mechanosensitive TRP channels in osteoblasts. (2009)
  • Author(s): Takashi Yoshida, Yuki Miyajima, Minoru Wakamori.
  • Organizer: The 3rd Inernational Symposium for Inerface Oral Health Science
  • Place of Presentation: 仙台
  • Year and Date: 2009-01-16
• [Presentation] Expression patterns of mechanosensitive TRP channels in ost eoblasts (2009)
  • Author(s): Takashi Yoshida, Yuki Miyajima, Minoru Wakamori
  • Organizer: The 3rd Inernational Symposium for Inerface Oral Health Science
  • Place of Presentation: 仙台
  • Year and Date: 2009-01-16
• [Presentation] 外液カルシウムによるTRPC5チャネルの活性調節の解析 (2008)
  • Author(s): 若森実、吉田卓史
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-25
• [Presentation] 感覚センサーとして機能するイオンチャネルの探求 (2008)
  • Author(s): 吉田卓史
  • Organizer: 新世代の生物有機化学研究会2008
  • Place of Presentation: 名古屋
  • Year and Date: 2008-06-28