Action potential-dependent ATP release from taste receptor cells : development of real-time micro determination for neurotransmitter release at single ceII level

• Project/Area Number: 20791355
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Kyushu University
• Principal Investigator: MURATA Yoshihiro Kyushu University, 教育研究部・医療学系, 助教 (40377031)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 味細胞 / 活動電位 / シナプス / 神経伝達物質 / ATP / ヘミチャネル

Research Abstract

Subsets of taste bud cells generate action potentials in response to sapid stimuli. Type II taste cells have many taste transduction molecules but lack well elaborated synapses, which leads to the question in the functional significance of action potentials in these cells. We examined the dependence of adenosine triphosphate (ATP) transmitter release from taste cells upon action potentials. To identify Type TT taste cells we used mice expressing a green fluorescence protein (GFP) transgene from the gustducin promoter. Action potentials were recorded by an electrode basolaterally attached to a. single GFP-positive taste cell. ATP release was monitored from gustducin-expressing taste cells by collecting the electrode solution immediately after tastant-stimulated action potentials and using a. luciferase assay to quantify ATP. Stimulation of gustducin-expressing taste cells with a. sweet, bitter, or umami compound on the apical membrane increased ATP levels in the electrode solution; the amount of ATP depended on the firing rate. Increased spontaneous firing rates also induced ATP release from gustducin-expressing taste cells. ATP release from gustducin-expressing taste cells was depressed by tetrodotoxin and inhibited below the detection limit by carbenoxolone. Our data support the hypothesis that action potentials in Type II taste cells enhance ATP release through hemichannels.

Research Products

• [Journal Article] Discrimination of taste qualities among mouse fungi form taste bud cells (2009)
  • Author(s): Yoshi da. R, Mi yauchi A, Y2 Suo T, kotaki M, Murata. Y, Yasumatsu K, Shi gemura N, Yanagawa Y, Obata K, Ueno H, Margolskee RF, Ninomi ya Y
  • Journal Title: The Journal of Physiology Vol.587 Pages: 4425-4439
  • Peer Reviewed
• [Journal Article] Multiple receptors underlie glutamate taste responses in mice (2009)
  • Author(s): Yasumatsu K, Horio N, Murata. Y, Shirosaki S, Ohkuri T, Yoshida. R, Ninomiya Y
  • Journal Title: The American Journal of Clinical Nutrition Vol.90 Pages: 747-752
  • Peer Reviewed
• [Journal Article] Modulation and transmission of sweet taste information for energy homeostasis (2009)
  • Author(s): Sanematsu K, Horio N, Murata Y, Yoshida R, Ohkuri T, Shigemura N, Ninomiya Y
  • Journal Title: Annals of the New York Academy of Sciences Vol.1170 Pages: 102-106
  • Peer Reviewed
• [Journal Article] Multiple receptor systems for umami taste in mice (2009)
  • Author(s): Yoshida R, Yasumastsu K, Shirosaki S, Jyotaki M, Horio N, Murata Y, Shigemura N, Ninomiya Y
  • Journal Title: Annals of the New York Academy ofSciences Vol.1170 Pages: 51-54
  • Peer Reviewed
• [Journal Article] Contribution of the inositol 1, 4, 5-trisphosphate transduction cascade to the detection of "bitter" compounds in blowflies (2009)
  • Author(s): Ouyang Q, Sate H, Murata Y, Nakamura A, Ozaki M, Nakamura T
  • Journal Title: Comparative Biochemistry and Physiology PartA Vol.153 Pages: 309-316
  • Peer Reviewed
• [Journal Article] NaCl responsive taste cells in the mouse fungiform taste buds (2009)
  • Author(s): Yoshida R, Morio N, Murata Y, Yasumatsu K, Shigemura N, Ninomiya Y
  • Journal Title: Neuroseienee Vol.159 Pages: 795-803
  • Peer Reviewed
• [Journal Article] NaCl responsive taste cells in the mouse fungiform taste buds (2009)
  • Author(s): Ryusuke Yoshida, Nao Horio, Yoshihiro Murata, Noriatsu Shigemura, Yuzo Ninomiya
  • Journal Title: Neuroscience 159 Pages: 795-803
  • Peer Reviewed
• [Journal Article] Contribution of the inositol 1, 4, 5-trisphosphate transduction cascade to the detection of"bitter"compounds in blowflies (2009)
  • Author(s): Qin Ouyang, Hiroyasu Sato, Yoshihiro Murata, Atsushi Nakamura, Mamiko Ozaki, Tadashi Nakamura
  • Journal Title: Comparative Biochemistry and Physiology Part A 153 Pages: 309-316
  • Peer Reviewed
• [Journal Article] Multiple receptors underlie glutamate-taste responses in mice (2009)
  • Author(s): Keiko Yasumatsu, Nao Horio, Yoshihiro Murata, Shinya Shirosaki, Tadahiro Ohkuri, Ryusuke Yoshida, Yuzo Ninomiya
  • Journal Title: The American Journal of Clinical Nutrition 90 Pages: 747-752
  • Peer Reviewed
• [Journal Article] Discrimination of taste qualities among mouse fungiform taste bud cells (2009)
  • Author(s): Rysuke Yoshida, Aya Miyauchi, Toshiaki Yasuo, Masafumi Jyotaki, Yoshihiro Murata, Keiko Yasumatsu, Noriatsu Shigemura, Yuchio Yanagawa, Kunihiko Obata, Hiroshi Ueno, Robert F.Margolskee, Yuzo Ninomiya
  • Journal Title: The Journal of Physiology 257 Pages: 4425-4439
  • Peer Reviewed
• [Journal Article] Multiple receptor systems for umami taste in mice (2009)
  • Author(s): Ryusuke Yoshida, Keiko Yasumastsu, Shinya Shirosaki, Masafumi Jyotaki, Nao Horio, Yoshihiro Murata, Noriatsu Shigemura, Yuzo Ninomiya
  • Journal Title: Annals of the New York Academy of Sciences 1170 Pages: 51-54
  • Peer Reviewed
• [Journal Article] Modulation and transmission of sweet taste information for energy homeostasis (2009)
  • Author(s): Keisuke Sanematsu, Nao Horio, Yoshihiro Murata, Ryusuke Yoshida, Tadahiro Ohkuri, Noriatsu Shigemura, Yuzo Ninomiya
  • Journal Title: Annals of the New York Academy of Sciences 1170 Pages: 102-106
  • Peer Reviewed
• [Journal Article] 味細胞の細胞型と応答特性 (2008)
  • Author(s): 吉田竜介、村田芳博、安尾敏明、上瀧将史、柳川右千夫、小幡邦彦、植野洋志、Margolskee RF、二ノ宮裕三
  • Journal Title: 日本味と匂学会誌 15巻 Pages: 285-288
  • NAID: 110007369619
  • Peer Reviewed
• [Journal Article] マウスIT型味細胞の発火頻度依存性ATP放出 (2008)
  • Author(s): 村田芳博、吉田竜介、安尾敏明、柳川右千夫、小幡邦彦、植野洋志、Margolgkee RF、ニノ宮裕三
  • Journal Title: 日本味と匂学会誌 15巻 Pages: 381-384
  • Peer Reviewed
• [Journal Article] 味覚研究は生活習慣病対策のヒントを見つけ出せるか (2008)
  • Author(s): 村田芳博、二ノ宮裕三
  • Journal Title: G.I. Research 16巻 Pages: 388-395
• [Journal Article] マウスII型味細胞の発火頻度依存性ATP放出 (2008)
  • Author(s): 村田芳博, ほか
  • Journal Title: 日本味と匂学会誌 15 Pages: 381-384
  • NAID: 110007369646
  • Peer Reviewed
• [Journal Article] 味覚研究は生活習慣病対策のヒントを見つけ出せるか (2008)
  • Author(s): 村田芳博・ニノ宮裕三
  • Journal Title: G. I. Research 16 Pages: 388-395
  • Peer Reviewed
• [Journal Article] Action potential-enhanced ATP release from taste cells through hemichannels
  • Author(s): Murata. Y, Yasuo T, Yoshida. R, Ohata. K, Yanagawa Y, Margolskee RF, Ninomiya Y
  • Journal Title: Journal of Neurophy giology (印刷中)
  • Peer Reviewed
• [Presentation] 味細胞の神経伝達物質Arp放出における活動電位の機能的役割 (2010)
  • Author(s): 村田芳博
  • Organizer: 第1回味覚健康科学シンポジウム
  • Place of Presentation: なないろの風(大分県由布市)
  • Year and Date: 2010-03-13
• [Presentation] 味細胞の神経伝達物質ATP放出における活動電位の機能的役割 (2010)
  • Author(s): 村田芳博
  • Organizer: 味覚健康科学シンポジウム2009
  • Place of Presentation: なないろの風(大分県)
  • Year and Date: 2010-03-13
• [Presentation] 味細胞が神経伝達物質ATPを放出するメカニズム-味細胞が発する活動電位の機能的役割- (2010)
  • Author(s): 村田芳博、安尾敏明、吉田竜介、小幡邦彦、柳川右千夫、Margolgkee RF、二ノ宮裕三
  • Organizer: 第9回KMS Research Meeting
  • Place of Presentation: 高知大学医学部(高知県南国市)
  • Year and Date: 2010-02-04
• [Presentation] 味細胞が神経伝達物質ATPを放出するメカニズム-味細胞が発する活動電位の機能的役割- (2010)
  • Author(s): 村田芳博、安尾敏明、吉田竜介、小幡邦彦、柳川右千夫、Robert F.Margolskee、二ノ宮裕三
  • Organizer: 第9回KMS Research Meeting
  • Place of Presentation: 高知大学医学部(高知県)
  • Year and Date: 2010-02-04
• [Presentation] Action potential-dependent ATP release from Type II taste cells through hemichannels in mice (2009)
  • Author(s): Murata Y, Yasuo T, Yoshida R, Obata K, Yanagawa Y, Margolskee RF, Ninomiya Y
  • Organizer: Beijing International Meeting on Research in Taste and Smell
  • Place of Presentation: Marriot City Wall Hotel, Beijing, China
  • Year and Date: 2009-11-16
• [Presentation] Action potential-dependent ATP release from Type II taste cells through hemichannels in mice (2009)
  • Author(s): Yoshihiro Murata, Toshiaki Yasuo, Ryusuke Yoshida, Kunihiko Obata, Yuchio Yanagawa, Robert F.Margolskee, Yuzo Ninomiya
  • Organizer: Beijing International Meeting on Research in Taste and Smell
  • Place of Presentation: マリオットシティーウォールホテル(中国・北京市)
  • Year and Date: 2009-11-16
• [Presentation] Functional role of action potentials for ATP transmitter release from Type II taste cells (2009)
  • Author(s): Murata Y, Yasuo T, Yoshida R, Obata K, Yanagawa Y, Margolskee RF, Ninomiya Y
  • Organizer: The 7th Internat i onal Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
  • Place of Presentation: 九州大学馬出キャンパスコラボステーション(福岡県福岡市)
  • Year and Date: 2009-11-04
• [Presentation] Functional role of action potentials for ATP transmitter release from Type II taste cells (2009)
  • Author(s): Yoshihiro Murata, Toshiaki Yasuo, Ryusuke Yoshida, Kunihiko Obata, Yuchio Yanagawa, Robert F.Margolskee, Yuzo Ninomiya
  • Organizer: The 7th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
  • Place of Presentation: 九州大学馬出キャンパスコラボステーション(福岡県)
  • Year and Date: 2009-11-04
• [Presentation] 味細胞における味覚の受容と情報伝達 (2009)
  • Author(s): 吉田竜介、村田芳博、安松啓子、重村憲徳、ニノ宮裕三
  • Organizer: 第51回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 朱鷺メッセ(新潟県新潟市)
  • Year and Date: 2009-09-09
• [Presentation] 味細胞における味覚の受容と情報伝達 (2009)
  • Author(s): 吉田竜介、村田芳博、安松啓子、重村憲徳、二ノ宮裕三
  • Organizer: 第51回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 朱鷺メッセ(新潟県)
  • Year and Date: 2009-09-09
• [Presentation] Taste responsiveness of Type II and III taste bud cells in mouse fungiform papillae (2009)
  • Author(s): Yoshida R, Yasuo T, Murata Y, Jyotaki M, Yanagawa Y, Obata K, Ueno H, Damak S, Margolskee RF, Ninomiya Y
  • Organizer: The XXXVI International Congress of Physiological Seiences
  • Place of Presentation: 京都国際会議場(京都府京都市)
  • Year and Date: 2009-07-27
• [Presentation] Taste responsiveness of Type II and III taste bud cells in mouse fungiform papillae (2009)
  • Author(s): Ryusuke Yoshida, Toshiaki Yasuo, Yoshihiro Murata, Masafumi Jyotaki, Yuchio Yanagawa, Kunihiko Obata, Hiroshi Ueno, Sami Damak, Robert F.Margolskee, Yuzo Ninomiya
  • Organizer: The XXXVI International Congress of Physiological Sciences
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2009-07-27
• [Presentation] 味細胞の活動電位依存性ATP放出 (2009)
  • Author(s): 村田芳博
  • Organizer: 第13回鋤鼻研究会
  • Place of Presentation: 四季の湯温泉・ヘリテイジリゾート(埼玉県熊谷市)
  • Year and Date: 2009-06-13
• [Presentation] 味細胞の活動電位依存性ATP放出 (2009)
  • Author(s): 村田芳博
  • Organizer: 第13回鋤鼻研究会
  • Place of Presentation: ヘリテイジリゾート(埼玉県)
  • Year and Date: 2009-06-13
• [Presentation] 活動電位を発生する味細胞からのATP放出におけるヘミチャネルの関与 (2008)
  • Author(s): 村田芳博、吉田竜介、安尾敏明、柳川右千夫、小幡邦彦、植野洋志、Margolskee RF、二ノ宮裕三
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場(福岡県福岡市)
  • Year and Date: 2008-12-03
• [Presentation] マウスにおけるうま味の受容・伝達メカニズムの解析 (2008)
  • Author(s): 吉田竜介、安松啓子、城崎慎也、川東由利子、村田芳博、重村憲徳、中島清人、MarolskeeRF、二ノ宮裕三
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場(福岡県福岡市)
  • Year and Date: 2008-12-03
• [Presentation] Hemichannels involved in ATP release from taste cells with action potentials (2008)
  • Author(s): Yoshihiro Murata, et.al
  • Organizer: 日本生物物理学会第46年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-03
• [Presentation] II型味細胞は活動電位の頻度に依存してATPを放出する (2008)
  • Author(s): 村田芳博、安尾敏明、吉田竜介、二ノ宮裕三
  • Organizer: 第50回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: TOC有明(東京都江東区)
  • Year and Date: 2008-09-25
• [Presentation] GFPノックインマウスを用いたII型およびIII型味細胞の応答特製の解析 (2008)
  • Author(s): 吉田竜介、村田芳博、安尾敏明、上瀧将史、安松啓子、重村憲徳、二ノ宮裕三
  • Organizer: 第50回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: TOC有明(東京都江東区)
  • Year and Date: 2008-09-25
• [Presentation] II型味細胞は活動電位の頻度に依存してATPを放出する (2008)
  • Author(s): 村田芳博, ほか
  • Organizer: 第50回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-25
• [Presentation] 味細胞の細胞型と応答特性 (2008)
  • Author(s): 吉田竜介、村田芳博、安尾敏明、上瀧将史、柳川右千夫、小幡邦彦、植野洋志、MargolskeeRF、ニノ宮裕三
  • Organizer: 日本味と匂学会第42回大会
  • Place of Presentation: 富山市民プラザ(富山県富山市)
  • Year and Date: 2008-09-19
• [Presentation] マウスII型味細胞の発火頻度依存性ATP放出 (2008)
  • Author(s): 村田芳博, 吉田竜介, 安尾敏明, 柳川右千夫, 小幡邦彦, 植野洋志、MargolskeeRF, 二ノ宮裕三
  • Organizer: 日本味と匂学会第42回大会
  • Place of Presentation: 富山市民プラザ(富山県富山市)
  • Year and Date: 2008-09-18
• [Presentation] 甘味の受容・伝達・調節機構とATP (2008)
  • Author(s): 吉田竜介、村田芳博、安松啓子, 重村憲徳, 二ノ宮裕三
  • Organizer: 生理研研究会『病態と細胞外プリン-治療標的としての可能性を探る」
  • Place of Presentation: 自然科学研究機構岡崎コンファレンスセンター(愛知県岡崎市)
  • Year and Date: 2008-09-05
• [Presentation] Firing rate-dependent ATP release from mouse fungi form taste cell s with action potential s (2008)
  • Author(s): Murata. Y, Yoshi da R, Yasuo T, Yanagawa Y, Obata K, Ueno H, Margol skee RF, Ni nomi ya Y
  • Organizer: The 45th Internat i onal Symposium on Olfaction and Taste
  • Place of Presentation: Hyatt Regency, San Francisco, USA
  • Year and Date: 2008-07-25
• [Presentation] Temperature- and gurmarin-sensitivity of the chorda tympani nerve responses to sweetners in the wild-type, T1R3-, Ggust-, TRPM5-KO mice (2008)
  • Author(s): Ohkuri T, Yasumatsu K, Jyotaki M, Kusuhara Y, Yasuo T, Murata Y, Margolskee RF, Ninomiya
  • Organizer: The 15th International Symposium on Olfaction and Taste
  • Place of Presentation: Hyatt Regency, San Francisco, USA
  • Year and Date: 2008-07-25
• [Presentation] Firing rate-dependent ATP release from mouse fungiform taste cells with action potentials (2008)
  • Author(s): Yoshihiro Murata, et.al
  • Organizer: The 15th International Symposium 木 on Olfaction and Taste
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2008-07-25
• [Presentation] Modulation transmission of sweet taste information for energy homeostasis (2008)
  • Author(s): Ninomiya Y, Yoshida. R, Murata. Y, Yasumatsu K, Ohkuri T, Shirosaki S, Sanematsu K, Yasuo T, Nakamura Y, Shigemura N
  • Organizer: The 15th International Symposium on Olfaction and Taste
  • Place of Presentation: Hyatt Regency, San Francisco, USA
  • Year and Date: 2008-07-24
• [Presentation] Taste si gna 1 transmissicn froff fungi form taste bud cells to chorda tympani nerve fibers in mice (2008)
  • Author(s): Yoshi da, R, Murata Y, Yasuo T, Yasumatsu K, Shi gemura N, Ni nomi ya Y
  • Organizer: he 4 5t h Tnternational Symposium on 01 faction and Taste
  • Place of Presentation: Hyatt Regency, San Francisco, USA
  • Year and Date: 2008-07-22
• [Presentation] 味細部尾の細胞型により異なる応答特性 (2008)
  • Author(s): 吉田竜介、村田芳博、安尾敏明、上瀧将史、柳川右千夫、小幡邦彦、植野洋志、MargolskeeRF、二ノ宮裕三
  • Organizer: 第31回日本神経科学学会大会
  • Place of Presentation: 東京国際フォーラム(東京都千代田区)
  • Year and Date: 2008-07-11
• [Presentation] 発火頻度に依存したII型味細胞のATP放出 (2008)
  • Author(s): 村田芳博、吉田竜介、安尾敏明、柳川右千夫、小幡邦彦、植野洋志、MargolskeeRF、二ノ宮裕三
  • Organizer: 第31回日本神経科学学会大会
  • Place of Presentation: 東京国際フォーラム(東京都千代田区)
  • Year and Date: 2008-07-10
• [Presentation] ATP release from type II taste cells depending on firing rate (2008)
  • Author(s): Yoshihiro Murata, et.al
  • Organizer: 第31回日木神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Book] うま味受容とシグナル伝達 (2008)
  • Author(s): 村田芳博、二ノ宮裕三
• [Book] アミノ酸の科学と最新応用技術 (2008)
  • Author(s): 村田芳博・ニノ宮裕三
  • Publisher: シーエムシー出版