Biological specificity of endodontic pathogen E. faecalis in wide pH environment

• Project/Area Number: 20791381
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Tohoku University
• Principal Investigator: NAKAJO KAZUKO Tohoku University, 大学院・歯学研究科, 助教 (40374946)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯学 / 口腔細菌 / 腸内細菌 / E. faecalis / bifidobacteria / 感染根管 / pH耐性 / 歯内病巣 / 齲蝕病巣 / 生存活性 / 菌体内pH(pHi)維持能 / Enterococcus faecalis / Streptococcus mutans / 歯内う蝕病巣 / pH / アミノ酸 / 薬剤感受性

Research Abstract

The aim of this study was to evaluate the biological specificity in the wide pH environment of infected root canal-related bacteria : Enterococcus faecalis and Bifidobacterium longum and caries-related bacteria : Bifidobacterium dentium.E. faecalis grew well at wide pH range of arginine- containing complex medium, suggesting that this bacteria can grow using amino acid such as arginine in the exudates of persistent infected root canals where calcium hydroxide medicaments are ineffective or poor carbohydrate supply. Meanwhile, the high survival rate and intracellular pH maintenance ability of bifidobacteria comparable to Streptococcus mutans in the acidic environment may account for why bifidobacteria exist as stable species in acidic caries lesions together with mutans streptococci.

Research Products

• [Journal Article] Effects of antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on bacterial viability and metabolism. (2011)
  • Author(s): Izutani N, Imazato SIzutani S, Nakajo K, Takahashi N, Takahashi Y, Ebisu S, Russell RRB
  • Journal Title: Eur J Oral Sci 119(2) Pages: 175-181
  • Peer Reviewed
• [Journal Article] Effects of antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on bacterial viability and metabolism. (2011)
  • Author(s): Izutani N, Imazato S, Nakajo K, Takahashi N, Takahashi Y, Ebisu S, Russell RRB
  • Journal Title: European Journal of Oral Sciences Volume: 119 Pages: 175-181
  • Peer Reviewed
• [Journal Article] Resistance to acidic environments of caries-associated bacteria : Bifidobacterium dentium and Bifidobacterium longum. (2010)
  • Author(s): Nakajo K, Takahashi N, Beighton D
  • Journal Title: Caries Res 44(5) Pages: 431-437
  • Peer Reviewed
• [Journal Article] Resistance to acidic environments of caries-associated bacteria : Bifidobacterium dentium and Bifidobacterium longum. (2010)
  • Author(s): Nakajo K, Takahashi N Beighton D
  • Journal Title: Caries Research Volume: 44 Pages: 431-437
  • Peer Reviewed
• [Journal Article] Effects of alpha-amylase and its inhibitors on acid production from cooked starch by oral streptococci. (2009)
  • Author(s): Aizawa S, Miyasawa-Hori H, Nakajo K, Washio J, Mayanagi H, Fukumoto S, Takahashi N
  • Journal Title: Caries Res 43(1) Pages: 17-24
  • Peer Reviewed
• [Journal Article] Fluoride released from glass-ionomer cement is responsible to inhibit the acid production of caries-related oral streptococci. (2009)
  • Author(s): Nakajo K, Imazato S, Takahashi Y, Kiba W, Ebisu S, Takahashi N
  • Journal Title: Dental Materials 25(6) Pages: 703-708
  • Peer Reviewed
• [Journal Article] Effects of alpha-amylase and its inhibitors on acid production from cooked starch by oral streptococci (2009)
  • Author(s): Aizawa S, Miyasawa-Hori H, Nakajo K, Washio J, Mayanagi H, Fukumoto S, Takahashi N
  • Journal Title: Caries Research 43 Pages: 17-24
  • Peer Reviewed
• [Journal Article] Fluoride released from glassionomer cement is responsible to inhibit the acid production of cariesrelated oral streptococci (2009)
  • Author(s): Kazuko Nakajo
  • Journal Title: Dental Materials 25(印刷中)
  • Peer Reviewed
• [Presentation] Divalent cations enhance short-time fluoride exposure-induced inhibition on acid production by oral streptococci. (2011)
  • Author(s): Domon-Tawaraya H, Nakajo K, Washio J, Fukumoto S, Takahashi N
  • Organizer: The 4th International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai
  • Year and Date: 2011-03-08
• [Presentation] Actinomyces acid production : Effects on bicarbonate and fluoride at neural and acid pH. (2011)
  • Author(s): Kawashima J, Nakajo K, Washio J, Shimauchi H, Takahashi N
  • Organizer: The 4th International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai
  • Year and Date: 2011-03-08
• [Presentation] Acid-tolerance and endogenous acid-production of oral bifidobacteria. (2011)
  • Author(s): Nakajo K, Beighton D, Takahashi N
  • Organizer: The 4th International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai
  • Year and Date: 2011-03-08
• [Presentation] Quantification and identification of bacteria in the maxillary obturator-prostheses. (2011)
  • Author(s): Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Koyama S, Sasaki K, Takahashi N
  • Organizer: The 4th International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai
  • Year and Date: 2011-03-08
• [Presentation] Acid-tolerance and endogenous acid-production of oral bifidobacteria. (2011)
  • Author(s): Nakajo K, Beighton D, Takahashi N
  • Organizer: The 4th International Symposium for Interface Oral Health Science
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-08
• [Presentation] フッ化物局所応用によるプラーク齲蝕原性制御効果を再考する-プラーク細菌の酸産生抑制機構と二価金属イオンの影響- (2011)
  • Author(s): 中條和子
  • Organizer: 第3回「口腔環境制御研究」カテゴリー集会
  • Place of Presentation: 長崎
  • Year and Date: 2011-02-04
• [Presentation] Divalent cations enhance short-time fluoride exposure-induced inhibition on streptococcal acidogenicity. (2010)
  • Author(s): Domon-Tawaraya H, Nakajo K, Washio J, Fukumoto S, Takahashi N
  • Organizer: 第58回JADR学術大会
  • Place of Presentation: 北九州
  • Year and Date: 2010-11-20
• [Presentation] カルシウム・プレリンスはフッ化ナトリウム洗口によるプラーク酸産生抑制効果を増強する (2010)
  • Author(s): 田代宗嗣, 北村淳, 村岡希, 中條和子, 高橋信博
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-22
• [Presentation] 齲蝕関連細菌Bifidobacterium dentiumとBifidobacterium longumの耐酸性-ミュータンスレンサ球菌、乳酸桿菌との比較 (2010)
  • Author(s): 中條和子, 高橋信博, Beighton D
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-22
• [Presentation] 齲蝕関連細菌Bifidobacterium dentiumとBifidobacterium longumの耐酸性-ミュータンスレンサ球菌、乳酸桿菌との比較- (2010)
  • Author(s): 中條和子, 高橋信博, Beighton D
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-22
• [Presentation] 短時間フッ化物曝露によるStreptococcus mutans酸産生抑制効果に与える二価イオンの影響 (2010)
  • Author(s): 土門-俵谷ひと美, 中條和子, 鷲尾純平, 福本敏, 高橋信博
  • Organizer: 第52回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Presentation] Acid-tolerance of oral bifidobacteria and its comparison with Streptococcus mutans. (2010)
  • Author(s): Nakajo K, Yagishita Y, Takahashi N, Beighton D
  • Organizer: The 88th IADR
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-17
• [Presentation] 種々の濃度での抗菌性モノマーMDPBの殺菌・静菌特性の解析 (2009)
  • Author(s): 泉谷尚美、今里聡、中條和子、高橋信博、高橋雄介、恵比須繁之
  • Organizer: 日本歯科保存学会2009 年度秋季学術大会 第131回
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-29
• [Presentation] 種々の濃度での抗菌性モノマーMDPBの殺菌・静菌特性の解析 (2009)
  • Author(s): 泉谷尚美、今里聡、中條和子、高橋信博、高橋雄介、恵比須繁之
  • Organizer: 日本歯科保存学会2009年度秋季学術大会(第131回)
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-29
• [Presentation] Evaluation of inhibitory effect of biomaterials on oral biofilm : Ti-Ag alloys. Interdisciplinary Science of Nanomaterials (2009)
  • Author(s): Nakajo K, Takahashi M, Sasaki K, Takahashi N
  • Organizer: Japan-Korea Asian Core Program General Meeting in conjunction with Global COE Program International Conference 2009
  • Place of Presentation: Sendai
  • Year and Date: 2009-09-25
• [Presentation] Evaluation of inhibitory effect of biomaterials on oral biofilm : Ti-Ag alloys (2009)
  • Author(s): Nakajo K, Takahashi M, Sasaki K, Takahashi N.
  • Organizer: Interdisciplinary Science of Nanomaterials(Japan-Korea Asian Core Program General Meeting in conjunction with Global COE Program International Conference 2009)
  • Place of Presentation: Sendai
  • Year and Date: 2009-09-25
• [Presentation] Detection of bacteria in closed hollow obturators of maxillary prostheses. (2009)
  • Author(s): Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Koyama S, Sasaki K, Takahashi N
  • Organizer: The 2nd Meeting of IADR Pan Asian Pacific Federation (PAPF) and the 1st Meeting of IADR Asia/Pacific Region (APR)
  • Place of Presentation: Wuhan, China
  • Year and Date: 2009-09-24
• [Presentation] Detection of bacteria in closed hollow obturators of maxillary prostheses (2009)
  • Author(s): Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Koyama S, Sasaki K, Takahashi N
  • Organizer: The 2nd Meeting of IADR Pan Asian Pacific Federation(PAPF)and the 1st Meeting of IADR Asia/Pacific Region(APR)
  • Place of Presentation: Wuhan, China
  • Year and Date: 2009-09-24
• [Presentation] Ti-Ag合金の口腔内細菌に対する付着抑制効果 (2009)
  • Author(s): 中條和子、高橋正敏、高橋信博
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-11
• [Presentation] Detection of microorganisms from inside of acrylic resin prostheses. (2009)
  • Author(s): Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Koyama S, Sasaki K, Takahashi N
  • Organizer: The 13^<th> International College of Prosthodontists.
  • Place of Presentation: Cape Town, South Africa
  • Year and Date: 2009-09-10
• [Presentation] Actinomycesの糖代謝-フッ化物耐性とその重炭酸による影響 (2009)
  • Author(s): 川嶋順子、中條和子、鷲尾純平、島内英俊、高橋信博
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-10
• [Presentation] 短時間フッ化物曝露によるStreptococcus mutansのフッ素吸着及び酸産生能への影響 (2009)
  • Author(s): 土門-俵谷ひと美、中條和子、鷲尾純平、宮澤-堀はるみ、福本敏、高橋信博
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-10
• [Presentation] Detection of microorganisms from inside of acrylic resin prostheses (2009)
  • Author(s): Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Koyama S, Sasaki K, Takahashi N
  • Organizer: The 13th International College of Prosthodontists
  • Place of Presentation: Cape Town, South Africa
  • Year and Date: 2009-09-10
• [Presentation] 短時間フッ化物曝露による Streptococcus mutans のフッ素吸着及び酸産生能への影響 (2009)
  • Author(s): 土門-俵谷ひと美、中條和子、鷲尾純平、宮澤-堀はるみ、福本敏、高橋信博
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-10
• [Presentation] Actinomyces の糖代謝―フッ化物耐性とその重炭酸による影響 (2009)
  • Author(s): 川嶋順子、中條和子、鷲尾純平、島内英俊、高橋信博
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-10
• [Presentation] 顎義歯の中空型オブチュレーター(塞栓部)内部の細菌叢の解析 (2009)
  • Author(s): 竹内裕尚、中條和子、佐藤拓一、佐久間陽子、小山重人、佐々木啓一、高橋信博
  • Organizer: 第6回東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2009-06-16
• [Presentation] 試作Ti-Ag合金はバイオフィルム形成を抑制する (2009)
  • Author(s): 高橋正敏
  • Organizer: 第54回東北大学歯学会
  • Place of Presentation: 仙台
  • Year and Date: 2009-02-27
• [Presentation] Short-term effect of single NaFmouthrinse on glucoseinduced pH fall in dental plaque (2009)
  • Author(s): Kazuko Nakajo
  • Organizer: 第3回インターフェイス口腔健康科学国際シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2009-01-16
• [Presentation] Detection of viable bacterial cells in acrylic resin denture bases (2009)
  • Author(s): Yasuhisa Takeuchi
  • Organizer: 第3回インターフェイス口腔健康科学国際シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2009-01-16
• [Presentation] Experimental Ti-Ag alloys inhibit biofilm adhesion (2009)
  • Author(s): Masatoshi Takahashi
  • Organizer: 第3回インターフェイス口腔健康科学国際シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2009-01-16
• [Presentation] アクリルレジン製補綴物内部の微生物の検出 (2009)
  • Author(s): 竹内裕尚、中條和子、佐藤拓一、佐久間陽子、小山重人、佐々木啓一、高橋信博
  • Organizer: 平成21年度先端歯学スクール2009
  • Place of Presentation: 淡路島
• [Presentation] Detection of microorganisms from inside of acrylic resin prostheses. (2009)
  • Author(s): Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Koyama S, Sasaki K, Takahashi N
  • Organizer: ナノ・アモルファス材料および高機能インターフェイス科学シンポジウム
  • Place of Presentation: 蔵王,山形
• [Presentation] Short-term inhibition of plaque acidogenicity by NaF-mouthrinse and fluoride retention within dental plaque. (2009)
  • Author(s): Takahashi N, Nakajo K, Asanoumi T, Shibata A, Yagishita Y, Washio J
  • Organizer: ORCA 2009
  • Place of Presentation: Budapest, Hungary
• [Presentation] Detection of viable bacterial cells in acrylic resin denture bases. (2009)
  • Author(s): Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Sasaki K, Takahashi N
  • Organizer: The 3rd International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai
• [Presentation] Short-term effect of single NaF-mouthrinse on glucose-induced pH fall in dental plaque. (2009)
  • Author(s): Nakajo K, Asanoumi T, Shibata A, Yagishita Y, Kato K, Takahashi N
  • Organizer: The 3rd International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai
• [Presentation] Short-term effect of fluoride on acid production by Streptococcus mutans. (2009)
  • Author(s): Domon H, Nakajo K, Washo J, Miyasawa-Hori H, Fukumoto S, Takahashi N
  • Organizer: The 3rd International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai
• [Presentation] Experimental Ti-Ag alloys inhibit biofilm adhesion. (2009)
  • Author(s): Takahashi M, Nakajo K, Takahashi N, Sasaki K, Okuno O
  • Organizer: The 3rd International Symposium for Interface Oral Health Science
  • Place of Presentation: Sendai
• [Presentation] Short-term inhibition of plaque acidogenicity by NaF-mouthrinse and fluoride retention within dental plaque (2009)
  • Author(s): Takahashi N, Nakajo K, Asanoumi T, Shibata A, Yagishita Y, Washio J
  • Organizer: ORCA 2009
  • Place of Presentation: Budapest, Hungary
• [Presentation] 細菌代謝コントロールによる口腔疾患予防戦略-フッ化物が有する静菌作用とその生化学的メカニズム- (2008)
  • Author(s): 中條和子、土門ひと美、川嶋順子、柳下陽子、高橋信博
  • Organizer: 第50回歯科基礎医学会学術大会,サテライトシンポジウム「基礎と臨床を繋ぐ研究を求めて-口腔細菌研究の未来を見据えて-」
  • Place of Presentation: 東京
  • Year and Date: 2008-09-23
• [Presentation] 短時間フッ化物応用による齲蝕関連菌Streptococcus mutansの酸産生抑制効果 (2008)
  • Author(s): 土門ひと美、中條和子、宮澤-堀はるみ、福本敏、高橋信博
  • Organizer: 第50回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-23
• [Presentation] フッ化物洗口はプラークの酸産生を短時間において抑制する (2008)
  • Author(s): 柳下陽子、柴田哲伸、浅海友文、中條和子、高橋信博
  • Organizer: 第50回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-23
• [Presentation] 細菌代謝コントロールによる口腔疾患予防戦略-フッ化物が有する静菌作用とその生化学的メカニズム- (2008)
  • Author(s): 中條和子
  • Organizer: 第50回歯科基礎医学会学術大会サテライトシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-09-23
• [Presentation] Quantification and identification of bacteria within acrylic resin denture bases. (2008)
  • Author(s): Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Sasaki K, Takahashi N
  • Organizer: The 86th IADR
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-03
• [Presentation] Short-term effect of NaF-mouthrinse on glucose-induced pH fall in plaque. (2008)
  • Author(s): Nakajo K, Asanoumi T, Shibata A, Yagishita Y, Kato K, Takahashi N
  • Organizer: The 86th IADR
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-03
• [Presentation] Short-term effect of NaFmouthrinse on glucoseinduced pH fall in plaque (2008)
  • Author(s): Kazuko Nakajo
  • Organizer: 第86回国際歯科研究学会
  • Place of Presentation: カナダトロント
  • Year and Date: 2008-07-03
• [Presentation] Quantification and identification of bacteria within acrylic resin denture bases (2008)
  • Author(s): Yasuhisa Takeuchi
  • Organizer: 第86回国際歯科研究学会
  • Place of Presentation: カナダトロント
  • Year and Date: 2008-07-03
• [Presentation] アクリルレジン製の入れ歯(レジン床義歯)の内部に潜む細菌の解析 (2008)
  • Author(s): 竹内裕尚、中條和子、佐藤拓一、佐久間陽子、佐々木啓一、高橋信博
  • Organizer: 第5回東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2008-05-19
• [Presentation] 歯科用充填材料・グラスアイオノマーセメントは齲蝕の原因となる酸産生を抑制する (2008)
  • Author(s): 中條和子、高橋雄介、騎馬和歌子、今里聡、高橋信博
  • Organizer: 第5回東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2008-05-19
• [Presentation] 歯科用充填材料・グラスアイオノマーセメントは齢蝕の原因となる酸産生を抑制する (2008)
  • Author(s): 中條和子
  • Organizer: 第5回東北大学バイオサイエンスシンポジウムおよび第12回学際ライフサイエンスシンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2008-05-19
• [Presentation] アクリルレジン製の入れ歯(レジン床義歯)の内部に潜む細菌の解析 (2008)
  • Author(s): 竹内裕尚
  • Organizer: 第5回東北大学バイオサイエンスシンポジウムおよび第12回学際ライフサイエンスシンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2008-05-19
• [Book] Detection of viable bacterial cells in acrylic resin denture bases.(Interface Oral Health Science 2009)(T.Sasano, O.Suzuki (eds.)) (2010)
  • Author(s): Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Sasaki K, Takahashi N
  • Publisher: Springer, New York
• [Book] Short-term effect of single NaF-mouthrinse on glucose-induced pH fall in dental plaque.(Interface Oral Health Science 2009)(T.Sasano, O.Suzuki (eds.)) (2010)
  • Author(s): Nakajo K, Asanoumi T, Shibata A, Yagishita Y, Kato K, Takahashi N
  • Publisher: Springer, New York
• [Book] Short-term effect of fluoride on acid production by Streptococcus mutans.(Interface Oral Health Science 2009)(T.Sasano, O.Suzuki (eds.)) (2010)
  • Author(s): Domon H, Nakajo K, Washo J, Miyasawa-Hori H, Fukumoto S, Takahashi N
  • Publisher: Springer, New York
• [Book] Experimental Ti-Ag alloys inhibit biofilm adhesion.(Interface Oral Health Science 2009)(T.Sasano, O.Suzuki (eds.)) (2010)
  • Author(s): Takahashi M, Nakajo K, Takahashi N, Sasaki K, Okuno O
  • Publisher: Springer, New York
• [Patent(Industrial Property Rights)] バイオフィルム抑制型抗菌性チタン合金 (2008)
  • Inventor(s): 高橋正敏, 中條和子, 奥野攻, 高橋信博, 菊池聖史, 高田雄京, 佐々木啓一
  • Industrial Property Rights Holder: 高橋正敏, 中條和子, 奥野攻, 高橋信博, 菊池聖史, 高田雄京, 佐々木啓一
  • Industrial Property Number: 2008-294012
  • Filing Date: 2008-11-18