Observation of ultra-structures in human enamel and dentin by Ultra-high Voltage Electron Tomography

• Project/Area Number: 20791384
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: JIRO Miura Osaka University, 歯学部附属病院, 助教 (70437383)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 石灰化硬組織 / 電子線トモグラフィー / ナノテクノロジー / 硬組織 / トモグラフィー / コラーゲン / 非脱灰観察

Research Abstract

Human enamel and dentin are hard and brittle mineralized tissues. It is difficult to prepare thin specimens (under 200 nm) of these tissues for transmission electron microscope observation without demineralizing them. We present a novel method for creating three-dimensional ultra structural images of human enamel and dentin using Focus Ion Beam method and ultra-high voltage electron microscope tomography.

Research Products

• [Journal Article] Multi-scale analysis of stress distribution in teeth under applied forces. (2009)
  • Author(s): Miura, J, Nakai H, Maeda Y, Zako M.
  • Journal Title: Dent Mater. 25 Pages: 67-73
  • Peer Reviewed
• [Journal Article] 人歯象牙質とボンディング層界面のトモグラフィー観察 (2009)
  • Journal Title: 阪大複合機能ナノファウンダリ研究成果報告書 第3巻 Pages: 109-110
  • Peer Reviewed
• [Journal Article] 超高圧電子顕微鏡を用いた歯科修復材料と象牙質の接着界面微細構造観察 (2009)
  • Author(s): 三浦治郎
  • Journal Title: 阪大複合機能ナノファウンダリ研究成果報告書 2巻 Pages: 66-67
• [Journal Article] Multi-scale analysis of stress distribution in teeth under applied forces (2009)
  • Author(s): Jiro Miura
  • Journal Title: Dental Materials 25 Pages: 67-73
  • Peer Reviewed
• [Journal Article] Biomechanical model of incisor avulsion: a preliminary report (2008)
  • Author(s): Miura, J. Maeda, Y.
  • Journal Title: Dent Traumatol. 24 Pages: 454-457
  • Peer Reviewed
• [Journal Article] 超高圧電子顕微鏡を用いた歯科修復材料と象牙質の接着界面微細構造観察 (2008)
  • Journal Title: 阪大複合機能ナノファウンダリ研究成果報告書 第2巻 Pages: 66-67
  • Peer Reviewed
• [Journal Article] Biomechanical model of incisor avulsion : a preliminary report (2008)
  • Author(s): Jiro Miura
  • Journal Title: Dental Traumatology 24 Pages: 454-457
  • Peer Reviewed
• [Presentation] マルチスケール解析を用いた歯の破折メカニズムの解明 (2009)
  • Author(s): 三浦治郎、長島正、竹重文雄
  • Organizer: 日本保存歯科学会(仙台)
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-29
• [Presentation] 超高圧電子顕微鏡を用いた修復材料と象牙質の接着界面微細構造観察 (2009)
  • Author(s): 三浦治郎、長島正、竹重文雄、森博太郎
  • Organizer: 日本保存歯科学会(仙台)
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-29
• [Presentation] 超高圧電子線トモグラフィーを用いた人歯長微細構造観察手法の開発 (2009)
  • Author(s): 三浦治郎、長谷川紀昭、竹重文雄、鷹岡昭夫
  • Organizer: 日本顕微鏡学会
  • Place of Presentation: 仙台
  • Year and Date: 2009-05-25
• [Presentation] マルチスケール応力解析を用いた歯の破折現象の解明 (2009)
  • Author(s): 三浦治郎
  • Organizer: 総合歯科医療学会
  • Place of Presentation: 千葉 松戸
• [Presentation] 超高圧電子顕微鏡を用いた修復材料と象牙質の接着界面微細構造観察 (2009)
  • Author(s): 三浦治郎
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 仙台
• [Presentation] マルチスケール解析を用いた歯の破折メカニズムの解明 (2009)
  • Author(s): 三浦治郎
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 仙台
• [Presentation] 超高圧電子線トモグラフィーを用いた人歯長微細構造観察手法の開発 (2008)
  • Author(s): 三浦治郎、長谷川紀昭、竹重文雄、森博太郎
  • Organizer: 日本保存歯科学会
  • Place of Presentation: 富山
  • Year and Date: 2008-11-07
• [Presentation] Observations of ultrastructures in human-tooth by Ultra-High Voltage Electron Tomography (2008)
  • Author(s): Jiro Miura
  • Organizer: International Association for Dental research
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-05
• [Presentation] Hirotaro Mori Observations of ultrastructures in human-tooth by Ultra-High Voltage Electron Tomography International Association for Dental research (2008)
  • Author(s): Jiro Miura, Noriaki Hasegawa
  • Place of Presentation: Toronto Canada
  • Year and Date: 2008-07-04
• [Book] 阪大複合機能ナノファウンダリ研究成果報告書第1巻 超高圧電子線トモグラフィーを用いた歯牙における非脱灰試料の超微細構造解析 (2008)
  • Author(s): 三浦治郎
  • Total Pages: 137
  • Publisher: 大阪大学産業科学研究所阪大複合機能ナノファウンダリ