Observation of ultra-structures in human enamel and dentin by Ultra-high Voltage Electron Tomography
| Project/Area Number |
20791384
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
JIRO Miura Osaka University, 歯学部附属病院, 助教 (70437383)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 石灰化硬組織 / 電子線トモグラフィー / ナノテクノロジー / 硬組織 / トモグラフィー / コラーゲン / 非脱灰観察 |
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| Research Abstract |
Human enamel and dentin are hard and brittle mineralized tissues. It is difficult to prepare thin specimens (under 200 nm) of these tissues for transmission electron microscope observation without demineralizing them. We present a novel method for creating three-dimensional ultra structural images of human enamel and dentin using Focus Ion Beam method and ultra-high voltage electron microscope tomography.
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Report
(3 results)
Research Products
(17 results)
