Identification of genes related to the regeneration of human periodontalligament tissue

• Project/Area Number: 20791387
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Kyushu University
• Principal Investigator: SHINSUKE Fujii Kyushu University, 歯内治療科, 助教 (60452786)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ヒト / 歯根膜 / 再生

Research Abstract

In this study, the purpose is to identify the genes that is related to the regeneration of human periodontal ligament tissue. In my laboratory, two undifferentiated PDL stem cell lines had been established, and had been named as cell line 1-11 or cell line 1-17. I identified 2420 genes of which expression level was different between these two cell lines. Of them, Transforming growth factor-β1(TGF- β1) was cultured with line 1-11. TGF-β1 suppressed the proliferation of line 1-11 and promoted the gene expression of type I collagen, fibrillin-1 and alpha smooth muscle actin in line 1-11.

Research Products

• [Journal Article] Effect of MTAD on the differentiation of osteoblast-like cells. (2010)
  • Author(s): Yasuda Y, Tatematu Y, Fujii S, Maeda H, Akamine A, Torabinejad M, Saito T
  • Journal Title: Journal of Endodontics. 36巻 Pages: 260-263
  • Peer Reviewed
• [Journal Article] Effect of MTAD on the differentiation of osteoblast-like cells (2010)
  • Author(s): Yasuda, et al.
  • Journal Title: Journal of Endodontics. 36 Pages: 260-263
  • Peer Reviewed
• [Presentation] AngiotensinIIis involved in the loading signal in stretched human PDL cells. (2009)
  • Author(s): Monnouchi S, Maeda H, Fujii S, Tomokiyo A, Hori K, Akamine A
  • Organizer: 3rd Hiroshima Conference on Education and Science in Dentistry
  • Place of Presentation: 広島
  • Year and Date: 2009-11-08
• [Presentation] AhRシグナルがヒト歯根膜細胞のコラーゲン代謝に及ぼす影響 (2009)
  • Author(s): 友清淳、前田英史、藤井慎介、和田尚久、門野内聡、堀清美、郡勝明、山本直秀、赤峰昭文
  • Organizer: 第131回日本歯科保存学会
  • Place of Presentation: 仙台
  • Year and Date: 2009-10-29
• [Presentation] TGFβ1がヒト歯根膜細胞および前駆細胞の増殖および分化に及ぼす影響 (2009)
  • Author(s): 藤井慎介、前田英史、友清淳、橋口勇、門野内聡、堀清美、和田尚久、赤峰昭文
  • Organizer: 第130回日本歯科保存学会
  • Place of Presentation: 札幌
  • Year and Date: 2009-06-11
• [Presentation] 伸展力が負荷されたヒト歯根膜細胞のシグナル伝達にはAngiotensinIIが関与している (2009)
  • Author(s): 門野内聡、前田英史、藤井慎介、友清淳、堀清美、赤峰昭文
  • Organizer: 第130回日本歯科保存学会
  • Place of Presentation: 札幌
  • Year and Date: 2009-06-11
• [Presentation] AhRシグナルがヒト歯根膜細胞のコラーゲン代謝に及ぼす影響 (2009)
  • Author(s): 友清淳、前田英史、藤井慎介、和田尚久、門野内聡、堀清美、内博史、古江増隆、赤峰昭文
  • Organizer: 全国油症会議
  • Place of Presentation: 福岡
  • Year and Date: 2009-06-08
• [Presentation] Expression and effects of TGF β-1 in periodontal ligament tissue. (2009)
  • Author(s): Fujii S, Maeda H, Wada N, Tomokiyo A, Monnouchi S, Hori K, Akamine A
  • Organizer: 87th General session & exhibition of the IADR/AADR/CADR
  • Place of Presentation: マイアミ米国
  • Year and Date: 2009-04-01
• [Presentation] MTA stimulates BMP2 expression in human PDL cells. (2009)
  • Author(s): Maeda H, Tomokiyo A, Fujii S, Monnouchi S, Wada N, Hori K, Akamine A
  • Organizer: 87th General session & exhibition of the IADR/AADR/CADR
  • Place of Presentation: マイアミ米国
  • Year and Date: 2009-04-01
• [Presentation] Expression and effects of TGF β-1 in periodontal ligament tissue. (2009)
  • Author(s): S Fujii, et al.
  • Organizer: 87^<th> general session & exhibition of the IADR/A ADR/CADR
  • Place of Presentation: マイアミ(米国)
  • Year and Date: 2009-04-01
• [Remarks]
  • URL: http://www.dent.kyushu-u.ac.jp/
• [Remarks]
  • URL: http://www.kyushu-u.ac.jp/
• [Remarks]
  • URL: http://www.kyushu-u.ac.jp/