| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Research Abstract |
A human osteoblast was cultured with α-MEM containing fish collagen (FC) that was total concentration of 0.1%(w/v). Osteoblast proliferation by cell count and the expression of mineralization-related genes and proteins with real-time PCR and western blot analyses were investigated. Furthermore, von Kossa staining was performed to study the effect of FC on the osteoblast at the prolonged culture. The results revealed that cell proliferation and mineralization-related genes and proteins increased in the FC group compared with control group. After the incubation of prolonged period, mineralized nodule of FC group augmented than that of control group.
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