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Dentin regeneration by dental pulp stem cells with adipokine

Research Project

Project/Area Number 20791390
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

YOSHIYUKI Yasuda  Health Sciences University of Hokkaido, 歯学部, 准教授 (80405670)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords象牙質再生 / 歯髄細胞 / アディポネクチン / 間葉系幹細胞
Research Abstract

Adiponectin is a blood hormone that is abundantly secreted by adipocytes, and is known to play an important role in the regulation of blood glucose levels through the mediation of adiponectin receptors 1 and 2 (AR1 and AR2, respectively). In this study, to evaluate the usefulness of adiponectin as a dental pulp-conserving agent, we investigated the function of adiponectin in dental pulp cells. RT-PCR analysis showed the expression of both AR1 and AR2 in dental pulp cells. Western blot analysis revealed AR1 protein expression in dental pulp cells. Alizarin Red staining showed that after the addition of 10μg/ml of adiponectin to the culture medium, the formation of mineralized nodules was significantly increased on Day 12. Treatment of dental pulp cells with adiponectin induced the expression of dentin sialophosphoprotein (DSPP) gene on Day 8. These results indicate that adiponectin promotes mineralization by inducing DSPP expression in dental pulp cells.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (18 results)

All 2009 2008

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (12 results)

  • [Journal Article] Carbon dioxide laser irradiation stimulates mineralization in rat dental pulp cells.2009

    • Author(s)
      Y. Yasuda, E. Ohtomo, T. Tsukuba, K. Okamoto, T. Saito
    • Journal Title

      Int Endod J 42

      Pages: 940-946

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adiponectin induces dentin sialophosphoprotein in rat dental pulp cells: an in vitro study.2009

    • Author(s)
      Y. Yasuda, T. Koike, T. Kawamorita
    • Journal Title

      J Endod 34

      Pages: 679-683

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Carbon dioxide laser irradiation stimulates mineralization in rat dentalpulp cells.2009

    • Author(s)
      Y.Yasuda, et al.
    • Journal Title

      Int Endod J 42

      Pages: 940-946

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adiponectin induces dentin sialophosphoprotein in rat dental pulp cells : an in vitro study2008

    • Author(s)
      Y. Yasuda, et.al.
    • Journal Title

      J Endod 34

      Pages: 679-683

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dentin phosphophoryn promotes cellular migration of human dental pulp cells2008

    • Author(s)
      Y. Yasuda, et.al.
    • Journal Title

      J Endod 34

      Pages: 575-578

    • NAID

      120006981302

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The effect of mineral trioxide aggregate on the mineralization ability of rat dental pulp cells : an in vitro study2008

    • Author(s)
      Y. Yasuda, et.al.
    • Journal Title

      J Endod 34

      Pages: 1057-1060

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 象牙質フォスフォフォリン由来ペプチドの石灰化への影響について2009

    • Author(s)
      安田善之,, ら
    • Organizer
      日本再生歯科医学会
    • Place of Presentation
      小倉
    • Year and Date
      2009-09-12
    • Related Report
      2009 Final Research Report
  • [Presentation] 象牙質フォスフォフォリン由来ペプチドの石灰化への影響について2009

    • Author(s)
      安田善之, ら
    • Organizer
      日本再生歯科医学会
    • Place of Presentation
      九州歯科大学(福岡)
    • Year and Date
      2009-09-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] 象牙質フォスフォフォリン由来ペプチドのヒト間葉系幹細胞の石灰化に与える影響2009

    • Author(s)
      安田善之,, ら
    • Organizer
      日本歯科基礎医学会
    • Place of Presentation
      新潟
    • Year and Date
      2009-09-10
    • Related Report
      2009 Final Research Report
  • [Presentation] 象牙質フォスフォフォリン由来ペプチドのヒト間葉系幹細胞の石灰化に与える影響2009

    • Author(s)
      安田善之, ら
    • Organizer
      日本歯科基礎医学会
    • Place of Presentation
      朱鷺メッセ(新潟)
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒト象牙質フォスフォフォリン由来ペプチドを用いた新規骨再生材料の開発2009

    • Author(s)
      安田善之,, ら
    • Organizer
      日本骨代謝学会
    • Place of Presentation
      大阪
    • Year and Date
      2009-07-23
    • Related Report
      2009 Final Research Report
  • [Presentation] ヒト象牙質フォスフォフォリン由来ペプチドを用いた新規骨再生材料の開発2009

    • Author(s)
      安田善之, ら
    • Organizer
      日本骨代謝学会
    • Place of Presentation
      大阪国際会議場(大阪)
    • Year and Date
      2009-07-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] MTAセメントは歯髄細胞のBMP-2発現を誘導する。2009

    • Author(s)
      安田善之,, ら
    • Organizer
      アジア歯内療法学会
    • Place of Presentation
      東京
    • Year and Date
      2009-04-25
    • Related Report
      2009 Final Research Report
  • [Presentation] MTAセメントは歯髄細胞のBMP-2発現を誘導する。2009

    • Author(s)
      安田善之, ら
    • Organizer
      アジア歯内療法学会
    • Place of Presentation
      都市センターホテル(東京)
    • Year and Date
      2009-04-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] 象牙質フォスフォフォリン由来ペプチドのヒト間葉系幹細胞の分化に与える影響2008

    • Author(s)
      安田善之, ら
    • Organizer
      日本歯科基礎医学会
    • Place of Presentation
      東京
    • Year and Date
      2008-09-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒト象牙質フォスフォフォリン由来ペプチドを用いた新規骨再生材料の開発2008

    • Author(s)
      安田善之, ら
    • Organizer
      日本再生歯科医学会
    • Place of Presentation
      東京
    • Year and Date
      2008-09-19
    • Related Report
      2008 Annual Research Report
  • [Presentation] Nd : YAGとEr : YAGレーザーによる根管内殺菌効果の比較検討2008

    • Author(s)
      安田善之, ら
    • Organizer
      日本歯科保存学会
    • Place of Presentation
      新潟
    • Year and Date
      2008-06-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] レーザーによる湾曲根管内消毒効果について2008

    • Author(s)
      安田善之, ら
    • Organizer
      日本歯内療法学会
    • Place of Presentation
      東京
    • Year and Date
      2008-05-24
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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