| Project/Area Number |
20791390
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
YOSHIYUKI Yasuda Health Sciences University of Hokkaido, 歯学部, 准教授 (80405670)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 象牙質再生 / 歯髄細胞 / アディポネクチン / 間葉系幹細胞 |
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| Research Abstract |
Adiponectin is a blood hormone that is abundantly secreted by adipocytes, and is known to play an important role in the regulation of blood glucose levels through the mediation of adiponectin receptors 1 and 2 (AR1 and AR2, respectively). In this study, to evaluate the usefulness of adiponectin as a dental pulp-conserving agent, we investigated the function of adiponectin in dental pulp cells. RT-PCR analysis showed the expression of both AR1 and AR2 in dental pulp cells. Western blot analysis revealed AR1 protein expression in dental pulp cells. Alizarin Red staining showed that after the addition of 10μg/ml of adiponectin to the culture medium, the formation of mineralized nodules was significantly increased on Day 12. Treatment of dental pulp cells with adiponectin induced the expression of dentin sialophosphoprotein (DSPP) gene on Day 8. These results indicate that adiponectin promotes mineralization by inducing DSPP expression in dental pulp cells.
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