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Analysis of bacterial biofilm in rat's root canal in vivo

Research Project

Project/Area Number 20791403
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionAichi Gakuin University

Principal Investigator

HIGUCHI Naoya  Aichi Gakuin University, 歯学部・歯学科, 講師 (10329609)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords細菌バイオフィルム / 根管 / バイオフィルム / Enterococcus feacalis / Pornhvromonas gingivalis / in vivo / ラット / 生体内
Research Abstract

First of all, I examined some growth conditions to form bacterial biofilm in vitro, using Enterococcus faecalis and Porphyromonas gingivalis. Then I tried to make bacterial biofilm in rat's root canal under the growth condition in vivo. As the result, I could not find any bacteria in all samples. I think that the cause was uncertain pulpectomy. Many immunity charge cells left in every root canal were killed all of bacteria. I suppose that biofilm formation in rat's root canal in vivo need continuous infection from bacterial flora.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (7 results)

All 2009 2008

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results)

  • [Journal Article] Immunocytochemical approach for surfac e layer proteins of freeze-substituted Tannerella forsythe nsis by energy-filt ering transmission electron microscopy2008

    • Author(s)
      K. Moriguchi, T. Jogahara, T. Kurihara, J. Iwami, N. Higuchi, Y. Murakami, H. Maeda, F. Yoshimura, H. Nakamura, N. Ohno
    • Journal Title

      Okajimas Folia Anatomica Japonica 85

      Pages: 67-72

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Presentation] 歯内療法において歯科用CTとマイクロスコープが有用であった1症例2009

    • Author(s)
      樋口直也、中田和彦、中村洋
    • Organizer
      愛知学院大学歯学会第74回学術大会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-06-14
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 歯内療法における歯科用CTとマイクロスコープの有用性:症例報告2009

    • Author(s)
      樋口直也、中田和彦、中村洋
    • Organizer
      第130回日本歯科保存学会春期学術大会
    • Place of Presentation
      北海道
    • Year and Date
      2009-06-11
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] Evaluation of Correspondence of Dental Computed Tomography Imaging to Anatomical Observation of External Root Resorption2009

    • Author(s)
      K. Nakata, I. Imaizumi, N. Higuchi, T. Watanabe, K. Inamoto, N. Shibata, H. Nakamura
    • Organizer
      The 15th Asian Pacific Endodontic Confederation. The 7th KAE & JEA, 30th JEA.
    • Place of Presentation
      Tokyo
    • Year and Date
      2009-04-25
    • Related Report
      2009 Final Research Report
  • [Presentation] Evaluation of Correspondence of Dental Computed Tomography Imaging to Anatomical Observation of External Root Resorption2009

    • Author(s)
      K.Nakata, I.Imaizumi, N.Higuchi, T.Watanabe, K.Inamoto, N.Shibata, H.Nakamura
    • Organizer
      The 15^<th> Asian Pacific Endodontic Confederation. The 7^<th> KAE & JEA, 30^<th> JEA
    • Place of Presentation
      東京
    • Year and Date
      2009-04-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] マイクロスコープ下で根管内破折器具を除去した症例2009

    • Author(s)
      樋口直也、中村洋
    • Organizer
      第6回日本顕微鏡歯科学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-04-19
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] 歯科用CTとマイクロスコープを併用した歯内療法2008

    • Author(s)
      中田和彦、安芸義朗、柴田直樹、今泉一郎、樋口直也、鈴木一吉、中村洋、泉雅浩、内藤宗孝、有地榮一郎
    • Organizer
      第21回日本歯科医学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2008-11-15
    • Related Report
      2009 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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