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Analysis of cis-reguratory elements and its ability to dedifferentiation of mature cells.

Research Project

Project/Area Number 20791461
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Dental engineering/Regenerative dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

NAKATA Hidemi  Tokyo Medical and Dental University, 歯学部・附属病院, 医員 (30451967)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords唇顎口蓋裂 / MSX1 / Msx1 / 脱分化 / 再生医療 / 遺伝子調節領域 / アデノウイルスベクター
Research Abstract

To analyze the ability of Msx1 to dedifferentiation in vitro and in vivo, we generated eGFP reported adeno viral vector which carried mouse msh-like1 (a homolog of human Msx1) cDNA and without containing msh-like1 sequences for control vector. After infection of these viral vectors to various mature cells, we extracted mRNA and RT-PCR was performed by RUNX2 for the marker of dedifferentiation and GAPDH for house keeping gene.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (3 results)

All 2009 2008

All Presentation (3 results)

  • [Presentation] Anterior tooth replacement with implants inalveolar cleft2009

    • Organizer
      18th EAO Annual ScientificMeeting Monaco
    • Related Report
      2009 Final Research Report
  • [Presentation] Anterior tooth replacement with implants in alveolar cleft sites2009

    • Author(s)
      Hidemi Nakata
    • Organizer
      EAO(18^(th) annual Scientific Meeting 2009)
    • Place of Presentation
      Monaco
    • Related Report
      2009 Annual Research Report
  • [Presentation] Clinical Evaluation on ImplantTreatment for CLP Patients2008

    • Organizer
      第53 回口腔外科学会総会
    • Place of Presentation
      徳島
    • Related Report
      2009 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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