Analysis of cis-reguratory elements and its ability to dedifferentiation of mature cells.
| Project/Area Number |
20791461
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NAKATA Hidemi Tokyo Medical and Dental University, 歯学部・附属病院, 医員 (30451967)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 唇顎口蓋裂 / MSX1 / Msx1 / 脱分化 / 再生医療 / 遺伝子調節領域 / アデノウイルスベクター |
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| Research Abstract |
To analyze the ability of Msx1 to dedifferentiation in vitro and in vivo, we generated eGFP reported adeno viral vector which carried mouse msh-like1 (a homolog of human Msx1) cDNA and without containing msh-like1 sequences for control vector. After infection of these viral vectors to various mature cells, we extracted mRNA and RT-PCR was performed by RUNX2 for the marker of dedifferentiation and GAPDH for house keeping gene.
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Report
(3 results)
Research Products
(3 results)
