An Experimental Study on Development of Cultured Epithelial Grafting Sheets with Amnion and Oral Epithelial Cells

• Project/Area Number: 20791462
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Shinshu University
• Principal Investigator: KOIKE Takeshi Shinshu University, 医学部附属病院, 助教 (60359734)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 移植 / 再生医療 / 再生医学 / 歯学 / 移植・再生医療

Research Abstract

The aim of this study was to assess the possibility of human oral mucosal epithelial cells on hyper-dry-amnion can be used as new source for culturing stratified epithelium sheets. The oral keratinocyte and hyper-dry-amnion composites had a well-stratified epithelial layer similar to native oral keratinized mucosa. Immunohistochemistry, the oral keratinocyte and hyper-dry-amnion composites appeared to be in a more proliferative state than native keratinized mucosa. These results suggested that the oral keratinocyte and hyper-dry-amnion composites may be a promising cell source for the development of stratified epithelium allograft sheets using a human cell strain.

Research Products

• [Journal Article] Cultured epithelial grafting using human amniotic membrane: The potential for using human amniotic epithelial cells as a cultured oral epithelium sheet. (2011)
  • Author(s): Koike T, Yasuo M, Shimane T, Kobayashi H, Nikaido T, Kurita H.
  • Journal Title: Arch Oral Biol. In press
  • Peer Reviewed
• [Journal Article] Cultured epithelial grafting using human amniotic membrane : The potential for using human amniotic epithelial cells as a cultured oral epithelium sheet. (2011)
  • Author(s): Koike T, Yasuo M, Shimane T, Kobayashi II, Nikaido T, Kurita II.
  • Journal Title: Arch Oral Biol. Volume: (In press)
  • Peer Reviewed
• [Presentation] An Experimental Study of Cultured Epithelial Grafting Using Human Amnion (2010)
  • Author(s): Takeshi Koike, Hiroichi Kobayashi, Tetsu Shimane, Hiroshi Kurita
  • Organizer: 88th General session and Exhibition of the International Association for Dental Research
  • Place of Presentation: Barcelona
• [Presentation] An Experimental Study of Cultured Epithelial Grafting Using Human Amnion. (2010)
  • Author(s): 小池剛史
  • Organizer: 第88回国際歯科学会
  • Place of Presentation: バルセロナ、スペイン
• [Presentation] ヒト乾燥羊膜と口腔上皮細胞による培養上皮シートの開発に関する実験的研究 (2009)
  • Author(s): 小池剛史、鎌田孝広、飯島響、小林啓一、栗田浩、倉科憲治
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌市
• [Presentation] ヒト乾燥羊膜と口腔上皮細胞による培養上皮シートの開発に関する実験的研究 (2009)
  • Author(s): 小池剛史
  • Organizer: 第54回日本口腔外科学会総会・学術大会
  • Place of Presentation: 扎幌
• [Presentation] ヒト乾燥羊膜と口腔上皮細胞による培養上皮シートの開発に関する実験的研究 (2008)
  • Author(s): 小池剛史、酒井宣紀、成川純之助、鎌田孝広、栗田浩、倉科憲治
  • Organizer: 第53回日本口腔外科学会総会
  • Place of Presentation: 徳島市
• [Presentation] ヒト乾燥羊膜と口腔 上皮細胞による培養上皮シートの開発に関する実験的研究 (2008)
  • Author(s): 小池剛史、酒井宣紀、酒井洋徳、小林啓一、栗田浩、倉科憲治
  • Organizer: 第62回日本口腔科学会総会
  • Place of Presentation: 福岡市
• [Presentation] ヒト乾燥羊膜と口腔上皮細胞による培養上皮シートの開発に関する実験的研究 (2008)
  • Author(s): 小池剛史
  • Organizer: 第53回日本口腔外科学会総会・学術大会
  • Place of Presentation: 徳島
• [Remarks]
  • URL: http://www.shinshu-u.ac.jp/faculty/medicine/chair/i-shika/kenkyu.html
• [Patent(Industrial Property Rights)] 培養重層上皮シートの改良作製方法 (2008)
  • Inventor(s): 二階堂敏雄、岡部素典、齋藤滋、倉科憲治、小池剛史
  • Industrial Property Rights Holder: 国立大学法人富山大学、国立大学法人信州大学
  • Industrial Property Number: 2008-106448
  • Filing Date: 2008-04-16