Study of involvement of sensory neurons in bone defect repair in rats with capsaicin-induced sensory denervation

• Project/Area Number: 20791488
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Tohoku University
• Principal Investigator: NAKAMURA Megumi 東北大学, 大学院・歯学研究科, 助教 (20431512)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥5,473,000 (Direct Cost: ¥4,210,000、Indirect Cost: ¥1,263,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 骨 / 修復 / 感覚神経 / カプサイシン / リモデリング / X線画像解析 / real-time PCR / ラット / 修復・再生

Research Abstract

We investigated bone repair in sensory-denervated rats. Nine-week-old male Wistar rats received subcutaneous injections of capsaicin to denervate sensory neurons. Rats treated with the same amount of vehicle served as controls. A standardized bone defect was created on the parietal bone. We measured the amount of repaired bone with quantitative radiographic analysis and the mRNA expressions of osteocalcin and cathepsin K with real-time PCR. The results suggested that capsaicin-induced sensory denervation affects the bone defect repair and remodeling.

Research Products

• [Journal Article] Remodeling of extracellular matrices initiates and advances calcification during development and healing of bones and teeth (2012)
  • Author(s): Sasano Y, Nakamura M, Okata H, Henmi A, Mikami Y
  • Journal Title: Journal of Oral Biosciences Volume: 54 Pages: 25-29
  • Peer Reviewed
• [Journal Article] Involvement of sensory neuron in bone defect repair in rats. (2011)
  • Author(s): Henmi A, Nakamura M, Echigo S, Sasano Y
  • Journal Title: J Electron Microsc Volume: 60(6) Pages: 393-400
  • Peer Reviewed
• [Journal Article] Root and periodontal tissue development after allogenic tooth transplantation between rat littermates. (2011)
  • Author(s): Andou K, Nakamura M, Ina Y, Sasaki K, Sasano Y
  • Journal Title: Oral Diseases Volume: 17
  • Peer Reviewed
• [Journal Article] Involvement of sensory neuron in bone defect repair in rats (2011)
  • Author(s): Henmi A, Nakamura M, 他
  • Journal Title: J Electron Microsc Volume: 60 Issue: 6 Pages: 393-400
  • DOI: 10.1093/jmicro/dfr075
  • Peer Reviewed
• [Presentation] 実験的骨欠損における修復骨形成過程の石灰化進行に関する検討 (2012)
  • Author(s): 大方広志、中村恵, 他
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 甲府市
• [Presentation] Effects of capsaicin treatment on nociception and structure of trigeminal nerve fibers in adult rats (2011)
  • Author(s): Akiko Kato, Megumi Nakamura, 他
  • Organizer: 第4回インターフェイス口腔健康科学国際シンポジウム
  • Place of Presentation: ホテル仙台プラザ(仙台市)
  • Year and Date: 2011-03-10
• [Presentation] Effects of capsaicin treatment on nociception and structure of trigeminal nerve fibers in adult rats (2011)
  • Author(s): Henmi A, Nakamura M, Echigo S, Sasano Y
  • Organizer: The 4th International Synposium for Interface Oral Health Science in Sendai
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2011-03-08
• [Presentation] Root elongation and periodontal tissue formation in tooth germs allogenically transplanted between rat littermates (2011)
  • Author(s): Andou K, Nakamura M, Ina Y, Sasaki K, Sasano Y
  • Organizer: The 4th International Synposium for Interface Oral Health Science in Sendai
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2011-03-08
• [Presentation] カプサイシンを利用した骨修復における感覚神経の役割の検討 (2010)
  • Author(s): 加藤晶子、中村恵、越後成志、笹野泰之
  • Organizer: 第115回日本解剖学会総会・全国学術集会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-03-28
• [Presentation] カプサイシンを利用した骨修復における感覚神経の役割の検討 (2010)
  • Author(s): 加藤晶子、中村恵, 他
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-28
• [Presentation] マイクロCTを利用した同腹他個体ラット間移植における歯根伸長の検討 (2009)
  • Author(s): 安藤浩二、中村恵、笹野泰之
  • Organizer: 第55回東北・北海道連合支部学術集会
  • Place of Presentation: 仙台市
  • Year and Date: 2009-09-26
• [Presentation] 同腹他個体ラット間の根未完成歯移植における歯根伸長と歯周組織形成に関する検討 (2009)
  • Author(s): 安藤浩二、中村恵、伊奈慶典、佐々木啓一、笹野泰之
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟市
  • Year and Date: 2009-09-11
• [Presentation] カプサイシンの全身投与が知覚および三叉神経線維の形態に及ぼす影響 (2009)
  • Author(s): 加藤晶子、中村恵, 他
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2009-09-10
• [Presentation] カプサイシンの全身投与が知覚および三叉神経線維の形態に及ぼす影響 (2009)
  • Author(s): 加藤晶子、中村恵、越後成志、笹野泰之
  • Organizer: 第51回歯科基礎医学会学術大会
  • Place of Presentation: 新潟市
• [Book] Effects of capsaicin treatment on nociception and structure of trigeminal nerve fibers in adult rats. (2012)
  • Author(s): Henmi A, Nakamura M, Echigo S, Sasano Y
  • Publisher: springer