The quantitative analysis of epigenetics for tailor-made cancer therapy

• Project/Area Number: 20791516
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: MATSUZAKI Hidenobu Okayama University, 岡山大学病院, 助教 (70325124)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 実験腫瘍学 / 臨床 / 薬理 / 遺伝子 / 発現制御

Research Abstract

In the present study, we examined the effects of FR901228, a histone deacetylase (HDAC) inhibitor, on epigenetic regulation of gene expression in oral cancer cell lines. The epigenetic status was quantified before and after FR901228 treatment via chromatin immuno-precipitation assay (ChIP). The methylation status was also quantitatively examined after 5-aza-dC/ FR901228 treatment. Moreover, the alterd-expression of genes related to angiogenesis and invasion may contribute to the recuperation of biological functions linked to FR901228 such as an inhibitory effect on tumor angiogenesis and cell invasion. These results indicate that FR901228 and its target genes may be excellent leads for future studies on the potential benefits of HDAC inhibitors and epigenetics in cancer therapy.

Research Products

• [Journal Article] A new phantom using polyethylene glycol as an apparent diffusion coefficient standard for MR imaging (2009)
  • Author(s): atsuya R, Kuroda M, Matsumoto Y, Kato H, Matsuzaki H, Asaumi J, Murakami JKatashima K, Ashida M, Sasaki T, Sei T, Himei K, Katsui K, Katayama N, Takemoto M, Kanazawa S, Mimura S, Oono S, Kitayama T, Tahara S, Inamura K
  • Journal Title: Int J Oncol ol.35、No.4 Pages: 893-900
  • Peer Reviewed
• [Journal Article] In vitro experimental study of the relationship between the apparent diffusion coefficient and changes in cellularity and cell morphology (2009)
  • Author(s): Katashima K, Ashida M, Sasaki T, Sei T, Kanazawa S, Mimura S, Oono S, Kitayama T, Tahara S, Inamura K
  • Journal Title: Oncol Rep Vol.22、No.3 Pages: 641-648
  • Peer Reviewed
• [Journal Article] A new phantom using polyethylene glycol as an apparent diffusion coefficient standard for MR imaging. (2009)
  • Author(s): Matsuya R, et al.
  • Journal Title: Int J Oncol. 35(4) Pages: 893900-893900
  • Peer Reviewed
• [Journal Article] In vitro experimental study of the relationship between the apparent diffusion coefficient and changes in cellularity and cell morphology. (2009)
  • Author(s): Matsumoto Y, et al.
  • Journal Title: Oncol Rep. 22(3) Pages: 641648-641648
  • Peer Reviewed
• [Presentation] 1, 4-dihydroxy-2-naphthoic acid(DHNA)投与による骨吸収抑制作用について (2009)
  • Author(s): 村上純, 畦坪輝寿, 松崎秀信, 柳文修, 浅海淳一
  • Organizer: 第50回日本歯科放射線学会総会
  • Place of Presentation: 大阪国際会議場(グランキュ-ブ大阪)
• [Presentation] 1,4-dihydroxy-2-naphthoic acid(DHNA)投与による骨吸収抑制作用について (2009)
  • Author(s): 村上純, 畦坪輝寿, 松崎秀信, 柳文修, 浅海淳一
  • Organizer: 第50回日本歯科放射線学会総会
  • Place of Presentation: 大阪国際会議場(グランキューブ大阪)
• [Presentation] 口腔癌細胞株に対する新規抗癌剤KNK437による血管新生阻害と癌浸潤阻害作用について (2008)
  • Author(s): 村上純
  • Organizer: 日本歯科放射線学会第49回学術大会総会
  • Place of Presentation: 名古屋
• [Book] Inc. Drug Resistant Neoplasms (2009)
  • Author(s): Jun Murakami, et al
  • Total Pages: 38
  • Publisher: Nova Science Publishers
• [Book] Drug Resistant Neoplasms (2009)
  • Author(s): Jun Murakami, et al.
  • Total Pages: 38
  • Publisher: Nova Science Publishers, Inc