Developomental study of molecular target therapy to oral cancer with FGFBP.
Project/Area Number |
20791519
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Surgical dentistry
|
Research Institution | Hiroshima University |
Principal Investigator |
SHINTANI Tomoaki Hiroshima University, 病院, 助教 (90403518)
|
Project Period (FY) |
2008 – 2009
|
Project Status |
Completed (Fiscal Year 2009)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 分子標的治療 / FGF結合蛋白 |
Research Abstract |
HBp17 siRNA was synthesized and transfected. Control scrambled siRNA was used. Quantitative RT-PCR analysis was performed. Then, we performed westernblotting analysis to compare with HBp17 in cell and culture media at 48hrs after transfection. Finaly growth assay was done. In HBp17-transfected cells, HBp17 mRNA expression was significantly decreased compared with control. HBp17 protein expression was also decreased in cell or culture media. In roliferation assay, growth of HBp17 siRNA trasfected-OSCC KA, UE and NA was inhibited.
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Report
(3 results)
Research Products
(8 results)