Basic research of molecular targeting therapy targeted to SP cells in oral cancer
Project/Area Number |
20791534
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Surgical dentistry
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Research Institution | Nagasaki University |
Principal Investigator |
YANAMOTO Souichi Nagasaki University, 病院, 講師 (10315260)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
Keywords | 口腔癌 / 癌幹細胞 / SP細胞 |
Research Abstract |
To determine whether human oral cancer cell lines contain a SP cell fraction, we performed flow cytometry analysis and sorting, followed by culturing in serum-free medium (SFM) using the SCC25 tongue cancer cell line. SCC25 cells contained 0.23% SP cells. The fraction of SP cells was available to grow in SFM cultures. SP cells showed higher mRNA expression of stem cell markers (ABCG2, Oct-4 and EpCAM) as compared with non-SP cells. Moreover, SP cells demonstrated more drug resistance to 5-FU, as compared with non-SP cells. The clone formation efficiency of SP cells was significantly higher than non-SP cells at an equal cell number.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] RNAi-mediated down-regulation of alpha-actinin-4 decreases invasion potential in oral squamous cell carcinoma2010
Author(s)
Yamada, S., Yanamoto, S., Yoshida, H., Yoshitomi, I., Kawasaki, G., Mizuno, A., Nemoto, TK.
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Journal Title
International Journal of Oral and Maxillofacial Surgery
Volume: 39
Pages: 61-67
NAID
Related Report
Peer Reviewed
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