| Project/Area Number |
20791537
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
ISHIDA Takayuki Kyushu University, 医学部・歯学部附属病院, 医員 (20404501)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | 口腔扁平上皮癌 / リンパ節転移 / VEGF / 低酸素 / MMP / 細胞培養 / 免疫染色 / VEFF |
|---|
| Research Abstract |
The human oral squamous cell carcinoma cell lines, HSC-2 and HSC-4 cultured under normaxia (20%O_2, 5%CO_2) and hypoxia (5%O_2, 5%CO_2, 37%). The expression of VEGF was assesed by immunohistocheical staining and RT-PCR. In imumunohisotcehiminal staining, the expression of VEGF-C was stronger under hypoxia rather than normaxia of each cell line. In RT-PCR, the expression of VEGF-C and MMP-9 were increase under hypoxia. Therefore this result suggests VEGF-C induced by hypoxia may increase ability of metasatsis in oral squamous cell carcinoma.
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