| Project/Area Number |
20791543
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Saitama Medical University |
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Principal Investigator |
ABE Takahiro Saitama Medical University, 医学部, 助教 (20383250)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ゾレドロン酸 / コルチコステロイド / RANKL / OPG / TCR / ゾレドロネート / osteoprotegerin / αβTCR / γδTCR |
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| Research Abstract |
The aim of this study was to examine, in a basic setting, changes in receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG), and the expression of T cell receptor (TCR) in mice treated with zoledronate (ZOL) and prednisolone (PSL) and lipopolysaccharide (LPS). C57BL/6, NOD-scid, and NOD-scid transplanted peripheral blood mononuclear cells (PBMC) were used. Peripheral blood samples were obtained to assay for OPG and RANKL. Femur and mandible were prepared for histomorphometric analysis. The microarchitecture of the bone samples was assessed by micro-CT. TCR expression was analyzed by immunohistochemistry. Histomorphometric analysis indicated that OS/BS and OV/BV were lowered by the administration of ZOL. Ob.S/BS and Oc.S/BS were decreased. In the C57BL/6 group taking both of ZOL and PSL, injection of LPS into the oral mucosa increased the levels of serum-RANKL. We showed that combined administration of ZOL with PSL decreased bone turn-over, and furthermore the combination causes a different pattern of TCR expression. These findings suggest that PSL and LPS have differential effects on bone metabolism and the immunoresponse to ZOL.
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