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Elucidation of the structural abnormality on all chromosomes using mapping array in oral cancer

Research Project

Project/Area Number 20791549
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionTokyo Dental College

Principal Investigator

YAMAMOTO Nobuharu  Tokyo Dental College, 歯学部 (60385185)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords舌扁平上皮癌 / ヘテロ接合性消失(LOH) / コピー数異常(CAN) / 一塩基多型(SNP) / 癌抑制遺伝子(TSG) / コピー数異常(CNA)
Research Abstract

[Purpose] We analyzed genomic copy number abnormality on all genomes in the squamous cell carcinoma of the tongue using Affymetrix GeneChipR Mapping 10K Array in this study and tried the identification of the new metastasis connection gene of the squamous cell carcinoma of the tongue. [Materials and a method] We used 20 squamous cell carcinoma of the tongue cases. We analyzed a metastasis to regional lymph node-positive case (pN(+)) 3 cases and a negative case (pN(-)) 2 cases by GeneChipR Mapping 10K Array for histopathology and detected genome copy number abnormality we were common to a pN(+) case as compared with a pN(-) case, and to be found and listed the metastasis connection gene candidates who were present in theatrical company rank. Furthermore, we inspected the mRNA manifestation situation of the candidate gene by quantitative Real-time PCR. [Results] In the region that we were common to a pN(+) case, and a genomic copy number increased, 16 regions, region that decreased were 32 regions. We listed 75 existing candidate genes on these regions. A ZDHHC14 gene did locus in the one 6q25.3 region of the region that a copy number specifically increased for a pN(+) case. As a result of having assayed the mRNA expression level of the gene, high-frequency manifestation sthenia was found (40% of 8/20 cases). Furthermore, the mRNA manifestation sthenia was significantly found in a pN(+) case (P = 0.019). [Conclusions] These results suggested the possibility that ZDHHC14 was a new metastasis of the squamous cell carcinoma of the tongue-related gene.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (9 results)

All 2009 2008 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (4 results)

  • [Journal Article] Expression of the FAM5C in tongue squamous cell carcinoma2009

    • Author(s)
      Kuroiwa Tsukasa, Yamamoto Nobuharu, Onda Takeshi, Shibahara Takahiko
    • Journal Title

      Oncology Reports 22巻

      Pages: 1005-1011

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of the FAM5C in tongue squamous cell carcinoma.2009

    • Author(s)
      Kuroiwa, T., Yamamoto, N., Onda, T., Shibahara, T.
    • Journal Title

      Oncology Reports 22

      Pages: 1005-1011

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 舌扁平上皮癌における高密度SNP Genotypingアレイを用いた全ゲノムコピー数とヘテロ接合性消失の解析2008

    • Author(s)
      黒岩司、山本信治、恩田健志、別所央城、藥師寺孝、片倉朗、高野伸夫、柴原孝彦
    • Journal Title

      日本口腔外科学会雑誌 54巻

      Pages: 316-322

    • NAID

      10027088647

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 舌扁平上皮癌における高密度SNP Genotypingアレイを用いた全ゲノムコピー数とヘテロ接合性消失の解析2008

    • Author(s)
      黒岩司、山本言治、恩田健志、別所央城、藥師寺孝、片倉朗、高野伸夫、柴原孝彦
    • Journal Title

      日本口腔外科学会雑誌 54

      Pages: 316-322

    • NAID

      10027088647

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Whole-genome analysis of loss of heterozygosity(LOH)using Affymetrix 10K SNP Mapping Array in tongue squamous cell carcinoma

    • Author(s)
      Tsukasa Kuroiwa, Nobuharu Yamamoto, Takahiko Shibahara
    • Journal Title

      International Journal of Oncology (投稿中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Aberrant expression of ZDHHC14 gene in human tongue squamous cell carcinoma2009

    • Author(s)
      Onda Takeshi, Yamamoto Nobuharu, Yakushiji Takashi, Takagi Ryo, Kamiyama Isao, Uchiyama Takeshi, Takano Nonuo, Shibahara Takahiko
    • Place of Presentation
      ベルリン(ドイツ)
    • Year and Date
      2009-09-20
    • Related Report
      2009 Final Research Report
  • [Presentation] Aberrant expression of ZDHHC14 gene in human tonguesquamous cell carcinoma2009

    • Author(s)
      Onda, T., Yamamoto, N., Yakushiji, T., Takagi, R., Kamiyama, I., Uchiyama, T., Takano, N., Shibahara, T.
    • Organizer
      第34回欧州臨床腫瘍学会
    • Place of Presentation
      ベルリン(ドイツ)
    • Year and Date
      2009-09-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] Analysis of copy number abnormality (CNA) and loss of heterozygosity (LOH) on whole genome using single nucleotide polymorphism (SNP) genotyping arrays in tongue squamous cell carcinoma2008

    • Author(s)
      Kuroiwa Tsukasa, Yamamoto Nobuharu, Onda Takeshi, Uchiyama Takeshi, Takano Nobuo, Shibahara Takahiko
    • Organizer
      第8回アジア口腔顎顔面外科学会
    • Place of Presentation
      バンコク(タイ)
    • Year and Date
      2008-11-04
    • Related Report
      2009 Final Research Report
  • [Presentation] Analysis of copy number abnormality(CNA)and loss of heterozygosity (LOH) on whole genome using single nucleotide polymorphism(SNP)genotyping arrays in tongue squamous cell carcinoma2008

    • Author(s)
      Kuroiwa T., Yamamoto N., Onda T., Uchiyama T., Takano N., Shibahara T.
    • Organizer
      第8回アジア口腔顎顔面外科学会
    • Place of Presentation
      バンコク(タイ)
    • Year and Date
      2008-11-04
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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