The effected of cancer gene therapy for Del 1 and Fas L

• Project/Area Number: 20791551
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Nihon University
• Principal Investigator: KITANO Hisataka Nihon University, 医学部, 専修医 (50424726)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 癌 / 遺伝子 / 蛋白質 / バイオテクノロジー

Research Abstract

FasL and FasL/E3D1 was made and transfected for a cancer cell. In this examination, the transfected cells were concentrated to FasL protein for ECM by Del1. Andmore, the protein was effected to apoptosis and, second gene transfer. It was shown that the organization could concentrate it without a composed domain of Del1 losing the function with various kinds of bioactive substances.

Research Products

• [Journal Article] The Extracellular Matr ix Protein Del1 Induces Apoptosis via its Epidermal Growth Factor Motif (2010)
  • Author(s): Hisataka Kitano, Shinichiro Kokubun, Chiaki Hidai
  • Journal Title: Biochemical and Biophysical Research C ommunications (BBRC). 393(4) Pages: 757-76
  • Peer Reviewed
• [Journal Article] The Extracellular Matrix Protein Dell Induces Apoptosis viaits Epidermal Growth Factor Motif. (2010)
  • Author(s): Hisataka Kitano, Shinichiro Kokubun, Chiaki Hidai.
  • Journal Title: Biochemical and Biophysical Research Communications 393(4) Pages: 757-761
  • Peer Reviewed
• [Journal Article] The Del1 deposition domain can immobilize 3α-hydroxysteroid dehydrogenase in the extracellular matrix without interfering with enzymatic activity (2009)
  • Author(s): Chiaki Hidai, Hisataka Kitano, Shinichiro Kokubun
  • Journal Title: Bioprocess Biosyst Eng. 32(5) Pages: 569-573
  • Peer Reviewed
• [Journal Article] The Dell deposition domain can immobilize 3 a -hydroxysteroi d dehydrogenase in the extracellular matrix without interferi ng with enzymatic activity (2008)
  • Author(s): Chiaki Hidai, Hisataka Kitano, Shinichiro Kokubun
  • Journal Title: Bioprocess Biosyst Eng (in press)
  • Peer Reviewed