| Project/Area Number |
20791553
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | The Nippon Dental University |
|---|
Principal Investigator |
YOSHIHITO Shimazu The Nippon Dental University, 生命歯学部, 講師 (10297947)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 口腔癌 / 脈管新生 / 脈管侵襲 / 免疫組織化学 / 3次元構築 / 歯学 / 病理学 / 口腔腫瘍 / 癌浸潤 / 転移 / 組織立体構築 / 3次元形態計測 / 舌癌 / 立体構築 / 細胞増殖 / 血管新生 / リンパ管 |
|---|
| Research Abstract |
The present study aimed at gaining 3D quantitative information about invasion front pattern, angiogenesis, and lymphangiogenesis in oral squamous cell carcinoma. During the approved 2-year-period, the P.I. developed the experimental protocol and procedures of histology-based 3D reconstruction comprising multiple immunolabeling of cancer constituents (e.g., carcinoma cells and blood/lymph endothelial cells), alignment and superposition of digitized images, and computer-assisted color segmentation of labeled targets. The reconstructed 3D images allow us to inspect visually and quantitatively the complex architecture of cancer lesions.
|
