Analysis of the mechanism of Calcium-sensing receptor activation in cementoblasts
| Project/Area Number |
20791605
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Periodontal dentistry
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
KANAYA Sousuke Tohoku University, 病院, 医員 (80375097)
|
|---|
| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | セメント芽細胞 / 細胞外カルシウム / FGF-2 / Calcium-sensing receptor / 細胞外Ca^<2+> |
|---|
| Research Abstract |
We found that elevated levels of extracellular Ca^<2+> increase fibroblast growth factor (FGF)-2 gene expression in cementoblasts. Pretreatment with a protein kinase A (PKA) inhibitor, H89, or an adenylate cyclase inhibitor, MDL-12330A, inhibited Ca^<2+>-stimulated Fgf-2 expression. In contrast, pretreatment with the protein kinase C (PKC) inhibitor GF-109203X or the phospholipase C (PLC) inhibitor U73122 did not affect the expression of Fgf-2 transcripts, suggesting that the increase in Fgf-2 expression was dependent on the PKA but not the PLC/PKC signaling pathway.
|
Report
(3 results)
Research Products
(9 results)
