The balance of MMPs/TIMPs in gingival tissues of gingival overgrowth

• Project/Area Number: 20791609
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Periodontal dentistry
• Research Institution: Niigata University
• Principal Investigator: NAKASONE Naohiro Niigata University, 医歯学総合病院, 医員 (20447634)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯肉増殖症 / tissue inhibitors of metalloproteinases (TIMPs) / metalloproteinases (MMPs) / 歯周炎 / calcium-channel blocker / tissueinhibitors of metalloproteinases (TIMPs) / Tissue inhibitors of metalloproteinases (TIMPs) / metalloproteinases(MMP) / Tissue inhibitors of metallonroteinases (TIMPs) / metallonroteinases (MMPs)

Research Abstract

We analyzed gene expression and protein localization of metalloproteinases (MMP)-1 and tissue inhibitors of metalloproteinases (TIMP)-1, -2, -3, and -4 in gingival tissues of gingival overgrowth and periodontitis. In particular, our data in TIMP-3 and -4 mRNA expression and protein localization accepted and published in Archives of Oral Biology.

Research Products

• [Journal Article] Microarray and quantitative RT-PCR analyses in calcium-channel blockers induced gingival overgrowth tissues of periodontitis patients. (2011)
  • Author(s): Shimizu T, Kubota T, Nakasone N, Abe D, Morozumi T, Yoshie H
  • Journal Title: Arch Oral Biol 56 Pages: 277-284
• [Journal Article] Microarray and quantitative RT-PCR analyses in calcium-channel blockers induced gingival overgrowth tissues of periodontitis patients (2011)
  • Author(s): Shimizu T, Kubota T, Nakasone N, Abe D, Morozumi T, Yoshie H
  • Journal Title: Archives of Oral Biology Volume: 56 Pages: 277-84
  • Peer Reviewed
• [Journal Article] Altered gene expression in leukocyte transendothelial migration and cell communication pathways in periodontitis-affected gingival tissues. (2011)
  • Author(s): Abe D, Kubota T, Shimizu T, Morozumi T, Nakasone N, Yoshie H
  • Journal Title: Journal of Periodontal Research Volume: (in press)
  • Peer Reviewed
• [Journal Article] Differential gene and protein expression of tissue inhibitors of metalloproteinases (TIMP)-3 and TIMP-4 in gingival tissues from drug induced gingival overgrowth. (2009)
  • Author(s): Nakasone N, Kubota T, Hoshino C, Nohno K, Itagaki M, Shimizu T, Yoshie H
  • Journal Title: Arch Oral Biol 54 Pages: 634-641
• [Journal Article] Differential gene and protein expression of tissue inhibitors of metalloproteinases (TIMP)-3 and TIMP-4 in gingival tissues from drug induced gingival overgrowth (2009)
  • Author(s): Nakasone N, Kubota T, Hoshino C, Nohno K, Itagaki M, Shimizu T, Yoshie H
  • Journal Title: Archives of Oral Biology 54 Pages: 634-641
  • Peer Reviewed
• [Journal Article] The relationship between the cusp pattern and plural stem cell compartments in guinea pig cheek teeth by chasing BrdU-labeling (2009)
  • Author(s): Hashimoto E, Nakakura-Ohshima K, Kenmotsu S, Suzuki H, Nakasone N, Saito C, Harada H, Ohshima H
  • Journal Title: Archives of Histology and Cytology 71 Pages: 317-332
  • NAID: 10025096314
  • Peer Reviewed
• [Journal Article] The relationship between the cusp pattern and plural stem cell compartments in guinea pig cheek teeth by chasing BrdU-labeling. (2008)
  • Author(s): Hashimoto E, Nakakura-Ohshima K, Kenmotsu S, Suzuki H, Nakasone N, Saito C, Harada H, Ohshima H
  • Journal Title: Arch Histol Cytol 71 Pages: 317-332
  • NAID: 10025096314
• [Journal Article] Cell dynamics in the pulpal healing process following cavity preparation in rat molars. (2008)
  • Author(s): Harada M, Kenmotsu S, Nakasone N, Nakakura-Ohshima K, Ohshima H
  • Journal Title: Histochem Cell Biol 130 Pages: 773-783
• [Journal Article] A case report of multi-drug induced gingival overgrowth with TIMP-3 over-expression. (2008)
  • Author(s): Kubota T, Itagaki M, Morozumi T, Maruyama S, Nakasone N, Shimizu T, Saku T, Yoshie H
  • Journal Title: Oral Med Pathol 12 Pages: 141-148
• [Journal Article] A case report of multi-drug induced gingival overgrowth with TIMP-3 over-expression (2008)
  • Author(s): Kubota T, Itagaki M, Morozumi T, Maruyama S, Nakasone N, Shimizu T, Saku T, Yoshie H
  • Journal Title: Oral Medicine and Pathology 12 Pages: 141-148
  • Peer Reviewed
• [Journal Article] Cell dynamics in the pulpal healing process following cavity preparation in rat molars (2008)
  • Author(s): Harada M, Kenmotsu S, Nakasone N, Nakakura-Ohshima K, Ohshima H
  • Journal Title: Histochemistry and Cell Biology 130 Pages: 773-783
  • Peer Reviewed
• [Journal Article] Altered gene expression in leukocyte transendothelial migration and cell communication pathways in periodontitis-affected gingival tissues.
  • Author(s): Abe D, Kubota T, Shimizu T, Morozumi T, Nakasone N, Yoshie H
  • Journal Title: J Periodontal Res in press
• [Presentation] アルツハイマー病関連遺伝子は歯周炎罹患歯肉において転写発現亢進している (2010)
  • Author(s): 久保田健彦, 阿部大輔, 清水太郎, 中曽根直弘, 両角俊哉, 吉江弘正
  • Organizer: 第53回秋季日本歯周病学術大会
  • Place of Presentation: 徳島
  • Year and Date: 2010-09-20
• [Presentation] アルツハイマー病関連遺伝子は歯周炎罹患歯肉において転写発現亢進している (2010)
  • Author(s): 久保田健彦, 阿部大輔, 清水太郎, 中曽根直弘, 両角俊哉, 吉江弘正
  • Organizer: 第53回秋季日本歯周病学会
  • Place of Presentation: 徳島
  • Year and Date: 2010-09-20
• [Presentation] Alzheimer's disease pathway was accelerated in periodontitis-affected gingival tissues. (2010)
  • Author(s): Kubota T, Abe D, Shimizu T, Nakasone N, Morozumi T, Yoshie H
  • Organizer: International Association for Dental Research
  • Place of Presentation: Balcerona.
• [Presentation] Alzheimer's disease pathway was accelerated in periodontitis-affected gingival tissues (2010)
  • Author(s): Kubota T, Abe D, Shimizu T, Nakasone N, Morozumi T, Yoshie H
  • Organizer: International Association for Dental Research
  • Place of Presentation: バルセロナ
• [Presentation] Transcriptomes in sites with drug-induced gingival-overgrowth Microarray and qRT-PCR analyses. (2009)
  • Author(s): Shimizu T, Kuboota T, Abe D, Morozumi T, Nakasone N, Yoshie H
  • Organizer: EUROPERIO6
  • Place of Presentation: Stockholm.
• [Presentation] Comprehensive gene expression analysis of gingival tissues affected by localized chronic periodontitis. (2009)
  • Author(s): Abe D, Kuboota T, Shimizu T, Morozumi T, Nakasone N, Yoshie H
  • Organizer: EUROPERIO6
  • Place of Presentation: Stockholm.
• [Presentation] Comprehensive gene expression analysis of gingival tissues affected by localized chronic periodontitis (2009)
  • Author(s): Abe D, Kuboota T, Shimizu T, Morozumi T, Nakasone N, Yoshie H
  • Organizer: EUROPERIO6 (Poster presentations)
  • Place of Presentation: ストックホルム国際展示場(スウェーデン)
• [Presentation] Transcriptomes in sites with drug-induced gingival-overgrowth Microarray and qRT-PCR analyses (2009)
  • Author(s): Shimizu T, Kuboota T, Abe D, Morozumi T, Nakasone N, Yoshie H
  • Organizer: EUROPERIO6 (Poster presentations)
  • Place of Presentation: ストックホルム国際展示場(スウェーデン)
• [Presentation] カルシウム拮抗薬誘導性歯肉増殖症歯肉組織におけるtissue inhibitors of metalloproteinases (TIMP) -3, -4遺伝子発現およびタンパク質局在 (2008)
  • Author(s): 中曽根直弘, 久保田健彦, 星野千華, 板垣真奈美, 清水太郎, 吉江弘正
  • Organizer: 第51回秋季日本歯周病学術大会
  • Place of Presentation: 四日市
  • Year and Date: 2008-10-19
• [Presentation] カルシウム拮抗薬誘導性歯肉増殖症歯肉組織におけるTissue Inhibitors of Metalloproteinases(TIMP)-3, -4遺伝子発現およびタンパク質局在 (2008)
  • Author(s): 中曽根直弘, 久保田健彦, 星野千華, 板垣真奈美, 清水太郎, 吉江弘正
  • Organizer: 第51回秋季日本歯周病学術大会
  • Place of Presentation: 四日市
  • Year and Date: 2008-10-19
• [Remarks] ホームページ等