Delivery of siRNA to central nervous system viablood-brain barrirer transport : Therapy for prion disease

• Project/Area Number: 20800066
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Fukuoka University
• Principal Investigator: WATANABE Takuya Fukuoka University, 薬学部, 助教 (90509647)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: 薬学 / 感染症

Research Abstract

This study aimed to develop new therapy for prion diease without side effects, using a complex of siRNA and RVG-9R. The siRNA decreased expression of prion mRNA and prion protein of scrapie form (PrP^<Sc>). And siRNA/RVG-9R complex bound to cell. However, treatment of siRNA/RVG-9R complex did not affect PrP^<Sc> level in prion-infected cell culture. Sequence of RVG-9R needs to optimize for knock-down of prion.

Research Products

• [Journal Article] Lipopolysaccharide-activated microglia induce dysfunction of the blood-brain barrier in rat microvascular endothelial cells co-cultured with microglia. (2010)
  • Author(s): Sumi N, Nishioku T, Takata F, Matsumoto J, Watanabe T, Shuto H, Yamauchi A, Dohgu S, Kataoka Y.
  • Journal Title: Cell Mol Neurobiol. vol.30,No.2 Pages: 247-253
  • Peer Reviewed
• [Journal Article] Lipopolysaccharide-activated microglia induce dysfunction of the blood-brain barrier in rat microvascular endothelial cells co-cultured with microglia (2010)
  • Author(s): Sumi N, Nishioku T, Takata F, Matsumoto J, Watanabe T, Shuto H, Yamauchi A, Dohgu S, Kataoka Y.
  • Journal Title: Cell Mol Neurobiol. 30 Pages: 247-253
  • Peer Reviewed