Structural study of the polyglutamine aggregates

• Project/Area Number: 20810010
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: The University of Tokyo
• Principal Investigator: MATSUOKA Shigeru The University of Tokyo, 大学院・薬学系研究科, 助教 (60456184)
• Research Collaborator: INOUE Masayuki 東京大学, 大学院・薬学系研究科, 教授 (70322998) ; YAMAZAKI Toshio 理化学研究所, SSBC・チームリーダー (60273710)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥1,664,000 (Direct Cost: ¥1,280,000、Indirect Cost: ¥384,000); Fiscal Year 2009: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2008: ¥1,144,000 (Direct Cost: ¥880,000、Indirect Cost: ¥264,000)
• Keywords: 核磁気共鳴 / 有機合成 / ポリグルタミン病

Research Abstract

Uniform length short polyglutamine peptides (Q_5～Q_<10>) were synthesized and subjected to thioflavin T (ThT) stainning. Formation of ThT-stainable aggregate were observed for polyglutamines longer than 7 residues. Solid-state NMR showed short polyglutamine peptides adopts β-sheet structures.

Research Products

• [Journal Article] The First F-ring Modified Ciguatoxin Analogue Showing Significant Toxicity (2010)
  • Author(s): Yuuki Ishihara, Nayoung Lee, Naomasa Oshiro, Shigeru Matsuoka, Shuji Yamashita, Masayuki Inoue, Masahiro Hirama
  • Journal Title: Chem.Commun. 46 Pages: 2968-2970
  • Peer Reviewed
• [Journal Article] Variability in C_3-Plant Cell-Wall Biosynthesis in a High-CO_2 Atmosphere by Solid-State NMR (2010)
  • Author(s): Tsyr-Yan Yu, Manmilan Singh, Shigeru Matsuoka, Gary Patti, Gregory Potter, Jacob Schaefer
  • Journal Title: J.Am.Chem.Soc. 132 Pages: 6335-6341
  • Peer Reviewed
• [Journal Article] Overproduction, purification, crystallization and preliminary X-ray diffraction analysis of Trypanosoma brucei gambiense glycerol kinase (2010)
  • Author(s): Emmanuel Oluwadare Balogun, Daniel Ken Inaoka, Yasutoshi Kido, Tomoo Shiba, Takeshi Nara, Takashi Aoki, Teruki Honma, Akiko Tanaka, Masayuki Inoue, Shigeru Matsuoka, Paul A. M., Shigeharu Harada, Kiyoshi Kita
  • Journal Title: Acta Cryst. F66 Pages: 304-308
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic analysis of cyanide-insensitive alternative oxidase from Trypanosoma brucei brucei (2010)
  • Author(s): Yasutoshi Kido, Tomoo Shiba, Daniel Ken Inaoka, K. Sakamoto, Takeshi Nara, Takashi Aoki, Teruki Honma, Akiko Tanaka, Masayuki Inoue, Shigeru Matsuoka, Anthony Moore, Shigeharu Harada, Kiyoshi Kita
  • Journal Title: Acta Cryst. F66 Pages: 275-278
  • Peer Reviewed
• [Journal Article] Total Synthesis of the Large Non-Ribosomal Peptide Polytheonamide B (2010)
  • Author(s): Masayuki Inoue, Naoki Shinohara, Shintaro Tanabe, Tomoaki Takahashi, Ken Okura, Hiroaki Ito, Yuki Mizoguchi, Maiko Iida, Nayoung Lee, Shigeru Matsuoka
  • Journal Title: Nature Chem. 2 Pages: 280-285
  • Peer Reviewed
• [Journal Article] Importance of the Twisted Side-Chain of Antillatoxin for Potent Toxicity : Total Synthesis and Biological Evaluation of Antillatoxin and Analogues (2010)
  • Author(s): Ken Okura, Shigeru Matsuoka, Ryosuke Goto, Masayuki Inoue
  • Journal Title: Angew.Chem.Int.Ed. 49 Pages: 329-332
  • Peer Reviewed
• [Journal Article] Application of REDOR NMR in natural product chemistry (2009)
  • Author(s): Shigeru Matsuoka, Masayuki Inoue
  • Journal Title: Chem.Commun. 38 Pages: 5664-5675
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of aspartate carbamoyltransferase from a parasitic protist (2009)
  • Author(s): Kazuaki Matoba, Takeshi Nara, Takashi Aoki, Teruki Homma, Akiko Tanaka, Masayuki Inoue, Shigeru Matsuoka, Daniel Ken Inaoka, Kiyoshi Kita, Shigeharu Harada
  • Journal Title: Trypanosoma cruzi.Acta Cryst. F65 Pages: 933-936
  • Peer Reviewed
• [Journal Article] Critical importance of the nine-membered F ring of ciguatoxin for potent bioactivity : total synthesis and biological evaluation of F-ring-modified analogues (2008)
  • Author(s): Masayuki Inoue, Nayoung Lee, Keisuke Miyazaki, Toyonobu Usuki, Shigeru Matsuoka, Masahiro Hirama
  • Journal Title: Angew.Chem.Int.Ed. 47 Pages: 8611-8614
  • Peer Reviewed
• [Journal Article] Vancomycin Derivative with Damaged d-Ala-d-Ala Binding Cleft Binds to Cross-linked Peptidoglycan in the Cell Wall of Staphylococcus aureus (2008)
  • Author(s): Sung Joon Kim, Shigeru Matsuoka, Gary J. Patti, Jacob Schaefer
  • Journal Title: Biochemistry 47 Pages: 3822-3831
  • Peer Reviewed
• [Journal Article] Self-Assembly of Amphotericin B is Probably Surrounded by Ergosterol ; Bimolecular Interactions as Evidenced by Solid State NMR and CD Spectra (2008)
  • Author(s): Yusuke Kasai, Nobuaki Matsumori, Yuichi Umegawa, Shigeru Matsuoka, Hiroyuki Ueno, Hiroki Ikeuchi, Tohru Oishi, Michio Murata
  • Journal Title: Chemistry Eur.J. 14 Pages: 1178-1185
  • Peer Reviewed
• [Presentation] 巨大ペプチド系天然物ポリセオナミドBの全合成と構造活性相関 (2010)
  • Author(s): 篠原直樹, 飯田真依子, 溝口友紀, 松岡茂, 井上将行
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-29
• [Presentation] アンチラトキシンを構造基盤とした光スイッチ分子の開発 (2010)
  • Author(s): 大倉健, 後藤良祐, 松岡茂, 井上将行
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-29
• [Presentation] ポリグルタミン-チオフラビンT分子複合体の構造解析 (2010)
  • Author(s): 村井元紀, 松岡茂, 井上将行
  • Organizer: 日本薬学会 第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] ポリグルタミン-チオフラビンT分子複合体の構造解析 (2010)
  • Author(s): 村井元紀、松岡茂、井上将行
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] 天然低分子化合物への固体NMRの応用 (2010)
  • Author(s): 松岡茂
  • Organizer: 平成21年度NMR外部利用事業報告会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-15
• [Presentation] ポリセオナミドを構造基盤とした人工機能分子の合成研究 (2009)
  • Author(s): 伊藤寛晃, 松岡茂, 井上将行
  • Organizer: 第28回メディシナルケミストリーシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-11-25
• [Presentation] トリパノソーマDHODの阻害剤の合成と複合体構造 (2009)
  • Author(s): 飯田真依子, 李羅榮, 上條真, 松岡茂, 井上将行, 本間光輝, 田中昭子, 稲岡ダニエル健, 北潔
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-28
• [Presentation] アンチラトキシン側鎖部の構造と機能 (2009)
  • Author(s): 大倉健, 後藤良祐, 松岡茂, 井上将行
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-26
• [Presentation] アンチラトキシン構造類縁体の網羅的合成 (2009)
  • Author(s): 後藤良祐, 菅原達也, 飯田真依子, 大倉健, 松岡茂, 井上将行
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-26
• [Presentation] ポリセオナミドを構造基盤とした人工機能分子の合成研究 (2009)
  • Author(s): 伊藤寛晃, 松岡茂, 井上将行
  • Organizer: 日本薬学会 第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-26
• [Presentation] アンチラトキシン類縁体の網羅的全合成と機能解析 (2008)
  • Author(s): 大倉健, 後藤良祐, 飯田真依子, 李羅榮, 松岡茂, 井上将行
  • Organizer: 第34回反応と合成の進歩シンポジウム
  • Place of Presentation: 京都
• [Presentation] 巨大ペプチド系天然物ポリセオナミドBの全合成 (2008)
  • Author(s): 篠原直樹, 田名部真太郎, 高橋友章, 溝口友紀, 大倉健, 伊藤寛晃, 飯田真依子, 李羅榮, 平間正博, 松岡茂, 井上将行
  • Organizer: 第50回天然有機化合物討論会
  • Place of Presentation: 福岡
• [Book] ソレル有機化学(第3章) (2009)
  • Author(s): 村田道雄, 石橋正己, 木越英夫, 佐々木誠 (監訳), 荒井孝義, 荒井緑, 有本博一, 石橋正己, 及川雅人, 大石徹, 木越英夫, 北将樹, 此木敬一, 佐竹真幸, 佐々木誠, 末永聖武, 早川一郎, 松岡茂, 松森信明, 村田道雄 (共訳)
  • Publisher: 東京化学同人