| Project/Area Number |
20810018
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Osaka University |
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Principal Investigator |
SHIMMA Shuichi Osaka University, 科学教育機器リノベーションセンター, 特任研究員 (30515896)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
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| Keywords | 質量分析 / 生体分子 / 構造解析 / 高エネルギー衝突解離 / 糖鎖 / 生理活性物質 / 糖ペプチド / リン脂質 / チャージリモートフラグメンテーション / リン酸化ペプチド |
|---|
| Research Abstract |
A multi-turn tandem time-of-flight (TOF) mass spectrometer (MULTUM-TOF/TOF) was used in this study. The instrument has two features. One is high precursor ion selectivity of the first TOF, which has much longer flight distance. Another is the elimination of fragment ions via post-source decay process. Components of post-source decay change its kinetic energy, therefore they cannot path through electric sectors. We performed the structural analysis of biomolecules using high-energy collision induced dissociation (HE-CID). I confirmed that I could obtain a lot of useful information to determine precise structure of biomolecules with small quantity of analyte. The HE-CID using MULTUM-TOF/TOF is a powerful tool to determine structure of novel bioactive substances in the future.
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