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Cultivation of methanogens using the coculture method.

Research Project

Project/Area Number 20810046
Research Category

Grant-in-Aid for Young Scientists (Start-up)

Allocation TypeSingle-year Grants
Research Field Environmental technology/Environmental materials
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

SAKAI Sanae  Japan Agency for Marine-Earth Science and Technology, 海洋・極限環境生物圏領域, ポストドクトラル研究員 (70512911)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,328,000 (Direct Cost: ¥2,560,000、Indirect Cost: ¥768,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,768,000 (Direct Cost: ¥1,360,000、Indirect Cost: ¥408,000)
Keywords分離・培養 / 嫌気性微生物 / メタン生成古細菌 / 共生
Research Abstract

We previously reported the isolation of novel methanogens by using a new cultivation method、 referred to as the coculture method. Here, we extended our coculture method to various anaerobic environmental samples. As a result, we successfully cultivated some uncharacterized methanogens in coculture enrichments and eventually isolated new methanogens, within the order Methanomicrobiales and Methanocellales.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (6 results)

All 2010 2009 2008

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (2 results)

  • [Journal Article] Methanocella arvoryzae sp. nov., a hydrogenotrophic methanogen, isolated from Italian rice field soil.2010

    • Author(s)
      Sanae Sakai, Ralf Conrad, Werner Liesack, Hiroyuki Imachi
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology (in press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Methanocella arvoryzae sp.nov., a hydrogenotrophic methanogen, isolated from Italian rice field soil2010

    • Author(s)
      Sanae Sakai
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cultivation of methanogens under low-hydrogen conditions by using the coculture method.2009

    • Author(s)
      Sanae Sakai, Hiroyuki Imachi, Yuji Sekiguchi, I-Cheng Tseng, Akiyoshi Ohashi, Hideki Harada, Yoichi Kamagata
    • Journal Title

      Applied and Environmental Microbiology 75

      Pages: 4892-4896

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cultivation of methanogens under low-hydrogen conditions by using the coculture method2009

    • Author(s)
      Sanae Sakai
    • Journal Title

      Applied and Environmental Microbiology 75

      Pages: 4892-4896

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Isolation and characterization of Rice Cluster I methanogens.2009

    • Author(s)
      Sanae Sakai, Ralf Conrad, Hiroyuki Imachi
    • Organizer
      12th International Symposium on Microbial Ecology ISME 12
    • Year and Date
      2009-08-18
    • Related Report
      2009 Final Research Report
  • [Presentation] Isolation and characterization of Rice Cluster I methanogens2008

    • Author(s)
      Sanae Sakai
    • Organizer
      12^<th> International Symposium on Microbial Ecology ISME 12
    • Place of Presentation
      オーストラリア,ケアンズ,コンベンションセンター
    • Year and Date
      2008-08-18
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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