Construction of DNA nanostructures for photoelectric conversion

• Project/Area Number: 20850031
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: University of Hyogo
• Principal Investigator: TAKADA Tadao University of Hyogo, 大学院・工学研究科, 助教 (60511699)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,276,000 (Direct Cost: ¥2,520,000、Indirect Cost: ¥756,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,716,000 (Direct Cost: ¥1,320,000、Indirect Cost: ¥396,000)
• Keywords: DNA / 光電デバイス / 光エネルギー / ナノテクノロジー / 遺伝子解析

Research Abstract

Polymerase extension reaction was used to construct the DNA nanostructures and introduce photoredox molecules into DNA. Triphosphate derivatives labeled with the redox-active molecules were synthesized and used to incorporate into DNA by polymerase extension reaction. Photoelectric conversion system based on DNA nanostructures was constructed by using this method.

Research Products

• [Journal Article] Fluorescent Analysis of Excess Electron Transfer through DNA (2010)
  • Author(s): Takada, T. ; Tanaka, C. ; Nakamura, M. ; Yamana, K.
  • Journal Title: Bioorg. Med. Chem. Lett. 20 Pages: 994-994
• [Journal Article] Syntheses and Fluorescence of RNA Conjugates Having Pyrene-Modified Adenosine and Nitrobenzene-Modified Uridine Base Pairs (2010)
  • Author(s): Fukuda, M. ; Nakamura, M. ; Takada, T. ; Yamana, K.
  • Journal Title: Tetrahedron Lett. 51 Pages: 1732-1732
• [Journal Article] Fluorescent Analysis of Excess Electron Transfer through DNA (2010)
  • Author(s): Takada, T., et al.
  • Journal Title: Bioorg.Med.Chem.Lett. 20 Pages: 994-997
  • Peer Reviewed
• [Journal Article] "Signal-On" Detection of DNA Hole Transfer at the Single Molecule Level (2009)
  • Author(s): Takada, T. ; Takeda, Y. ; Fujitsuka, M. ; Majima, T.
  • Journal Title: J. Am. Chem. Soc. 131 Pages: 6656-6656
• [Journal Article] RNA-Mediated Electron Transfer: Double Exponential Distance Dependence (2009)
  • Author(s): Maie, K. ; Miyagi, K. ; Takada, T. ; Nakamura, M. ; Yamana, K.
  • Journal Title: J. Am. Chem. Soc. 131 Pages: 13188-13188
• [Journal Article] Fluorescence Quenching Properties of Multiple Pyrene-Modified RNAs (2009)
  • Author(s): Maie, K. ; Nakamura, M. ; Takada, T. ; Yamana, K.
  • Journal Title: Bioorg. Med. Chem. 17 Pages: 4996-4996
• [Journal Article] Synthesis and characterization of deoxyuridine triphosphates labeled with pyrene (2009)
  • Author(s): Tanimizu, Y. ; Takada, T. ; Nakamura, M. ; Yamana, K.
  • Journal Title: Nucleic Acids Symp Ser (Oxf) 131
• [Journal Article] DNA ligation using photoremovable protecting groups (2009)
  • Author(s): Kawano, Y. ; Takada, T. ; Nakamura, M. ; Yamana, K.
  • Journal Title: Nucleic Acids Symp Ser (Oxf) 173
• [Journal Article] Synthesis and hybridization of oligonucleotides attached to a redox reporter via ethenyl linker at 5-position of pyrimidine base (2009)
  • Author(s): Hasegawa, Y. ; Nakamura, M. ; Takada, T. ; Yamana, K.
  • Journal Title: Nucleic Acids Symp Ser (Oxf) 145
• [Journal Article] Duplex formation of multiple pyrene-modified RNAs (2009)
  • Author(s): Fukuda, M. ; Nakamura, M. ; Takada, T. ; Yamana, K.
  • Journal Title: Nucleic Acids Symp Ser (Oxf) 133
• [Journal Article] Signal-On Detection of DNA Hole Transfer at the Single Molecule Level (2009)
  • Author(s): Takada, T., et al.
  • Journal Title: J.Am.Chem.Soc. 131 Pages: 6656-6657
  • Peer Reviewed
• [Presentation] Construction of a DNA monolayer possessing multiple chromophores on a gold surface by using polymerase extension reaction (2010)
  • Author(s): Takada, T.
  • Organizer: ACS 239th National Meeting
  • Place of Presentation: 米国
  • Year and Date: 2010-03-25
• [Presentation] ポリメラーゼ伸長反応によるDNAの光機能化 (2009)
  • Author(s): 渡辺小百合, 高田忠雄, 中村光伸, 山名一成
  • Organizer: 日本化学会第89春季年会(2009)
  • Place of Presentation: 日本大学
  • Year and Date: 2009-03-27
• [Presentation] レドックス修飾DNAアプタマー固定化チップによる生体分子の電気化学的検出 (2009)
  • Author(s): 林英理子・高田忠雄・中村光伸・山名一成
  • Organizer: 日本化学会第89春季年会講演予稿集
• [Presentation] ポリメラーゼ伸長反応によるDNAの光機能化 (2009)
  • Author(s): 渡辺小百合・高田忠雄・中村光伸・山名一成
  • Organizer: 日本化学会第89春季年会講演予稿集
• [Presentation] 電子ドナー・アクセプター修飾RNAの合成と性質 (2009)
  • Author(s): 真家賢治・中村光伸・高田忠雄・山名一成
  • Organizer: 日本化学会第89春季年会講演予稿集
• [Presentation] フェロセン共役核酸塩基の合成と性質 (2009)
  • Author(s): 長谷川裕介・高田忠雄・中村光伸・山名一成
  • Organizer: 日本化学会第89春季年会講演予稿集
• [Presentation] 光脱離性保護基を利用したDNA連結反応 (2009)
  • Author(s): 河野裕太・高田忠雄・中村光伸・山名一成
  • Organizer: 日本化学会第89春季年会講演予稿集
• [Presentation] ピレン修飾三リン酸誘導体の合成と評価 (2009)
  • Author(s): 谷水陽介・高田忠雄・中村光伸・山名一成
  • Organizer: 日本化学会第89春季年会講演予稿集
• [Presentation] RNA二本鎖上に構築されたピレン会合体 (2009)
  • Author(s): 福田稔・真家賢治・中村光伸・高田忠雄・山名一成
  • Organizer: 日本化学会第89春季年会講演予稿集