Development of gene FISH for environmental microbiology
| Project/Area Number |
20860009
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Tohoku University |
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Principal Investigator |
KUBOTA Kengo Tohoku University, 大学院・工学研究科, 助教 (80455807)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥3,341,000 (Direct Cost: ¥2,570,000、Indirect Cost: ¥771,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,781,000 (Direct Cost: ¥1,370,000、Indirect Cost: ¥411,000)
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| Keywords | FISH法 / 遺伝子 / 環境微生物 / 機能遺伝子 / microbial autecology / シングルセル / two-pass TSA-FISH法 / mcr遺伝子 / gap遺伝子 / aps遺伝子 / 視覚的検出技術 |
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| Research Abstract |
In this study, gene FISH is developed for environmental microbiology. Two-pass TSA-FISH with oligonucleotide or polynucleotide probes successfully developed for detection of single copy genes in prokaryotic cells. Application of the technique to environmental samples was also successful ; further application of this technique for environmental microbial autecology is possible.
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Report
(3 results)
Research Products
(14 results)
