| Project/Area Number |
20860031
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Intelligent mechanics/Mechanical systems
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
HOSHINO Takayuki Tokyo University of Agriculture and Technology, 大学院・工学府, 特任助教 (00516049)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,289,000 (Direct Cost: ¥2,530,000、Indirect Cost: ¥759,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,729,000 (Direct Cost: ¥1,330,000、Indirect Cost: ¥399,000)
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| Keywords | 細胞内電極 / 自己組織化 / 細胞運動 / ナノ電極 / ナノニードル / 集束イオンビーム / 細胞内計測 / 細胞接着 / 単一細胞計測 / 生体インターフェース |
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| Research Abstract |
We demonstrated the insertion of an intracellular nanoneedle into a single living cell which was caused by a cell-driven force. The cell-driven assembly of bio-hybrid micro and nanocomponents offers a potential route in nano-bioengineering research on new fabrication tools of bio-micro electro-mechanical systems (bio-MEMS) without direct mechanical manipulation. We describe the cell-driven self-insertion of a single conductive tungsten nanoneedle of 190nm diameter. A single nanoneedle was fabricated on the bottom of each 15μm wide glass microwell. A myoblast C2C12 cell migrated into a microwell; within 3hours, the insertion of a nanoneedle into the cell was observed using a three-dimensional laser scanning confocal microscope. Observation of fluorescence labeled viable cells indicated that no dead cells were present in the microwells during the nanoneedle insertion process. The intracellular nanoneedles were broken after a maximum of 2days.
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