The structural basis of the Fe-S cluster biosynthesis

• Project/Area Number: 20870002
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tohoku University
• Principal Investigator: YABE Toshiki Tohoku University, 多元物質科学研究所, 特別教育研究教員 (10451634)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 鉄硫黄蛋白質 / 葉緑体 / 鉄硫黄クラスター / 光化学系

Research Abstract

The biosynthesis of Fe-S clusters is a highly regulated process involving several proteins. To investigate the molecular basis of the Fe-S cluster biosynthesis pathway, we carried out the structural analysis of the holo form of chloroplast CnfU. Curve-fitting analysis of EXAFS indicated that the coordination environment of the [2Fe-2S] cluster on crystalline CnfU has a different environment from the environment at solution holo-CnfU. The structure of holo-CnfU supports a model in which CnfU has suitable structural feature for Fe-S cluster formation and transfer under an elevated oxygen environment.

Research Products

• [Journal Article] Arabidopsis cytosolic Nbp35 homodimer can assemble both [2Fe-2S] and [4Fe-4S] clusters in two distinct domains (2009)
  • Author(s): Hirokazu Kohbushi, 他
  • Journal Title: Biochemical and Biophysical Research Communications 378 Pages: 810-815
  • Peer Reviewed
• [Journal Article] Structural Analysis of Arabidopsis CnfU Protein : An Iron-Sulfur Cluster Biosynthetic Scaffold in Chloroplasts (2008)
  • Author(s): Toshiki Yabe, Eiki Yamashita, Akihiro Kikuchi, Kozo Morimoto, Atsushi Nakagawa, Tomitake Tsukihara, Masato Nakai
  • Journal Title: J.Mol.Biol. 381 Pages: 160-173
  • Peer Reviewed
• [Journal Article] Structural Analysis of Arabidopsis CnfU Protein : An Iron-Sulfur Cluster Biosynthetic Scaffold in Chloroplasts (2008)
  • Author(s): Toshiki Yabe, 他
  • Journal Title: Journal of Molecular Biology 381 Pages: 160-173
  • Peer Reviewed
• [Presentation] The structural basis of the iron-sulfur cluster biosynthesis in plant chloroplasts (2009)
  • Author(s): Toshiki Yabe, Masaki Unno, Akihiro Kikuchi, Eiki Yamashita, Toshitaka Matsui, Masato Nakai, Masao Ikeda-Saito
  • Organizer: ICBIC 14
  • Place of Presentation: Nagoya Congress Center, Nagoya, JAPAN
  • Year and Date: 2009-07-25
• [Presentation] The structural basis of the iron-sulfur cluster biosynthesis in plant chloroplasts (2009)
  • Author(s): Toshiki Yabe, 他
  • Organizer: ICBIC 14
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-25
• [Presentation] 鉄硫黄クラスター生合成機構の構造基盤 (2009)
  • Author(s): 矢部俊樹, 海野昌喜, 菊池晶裕, 山下栄樹, 松井敏高, 中井正人, 齋藤正男
  • Organizer: 第42回酸化反応討論会
  • Place of Presentation: 仙台
• [Presentation] The structural basis of the iron-sulfur cluster biosynthesis in plant chloroplasts (2009)
  • Author(s): Toshiki Yabe, Masaki Unno, Akihiro Kikuchi, Eiki Yamashita, Toshitaka Matsui, Masato Nakai, Masao Ikeda-Saito
  • Organizer: Gordon Research Conferences on Cell Biology of Metals
  • Place of Presentation: Salve Regina University, Newport, RI, USA
• [Presentation] 鉄硫黄蛋白質成熟化機構の構造生物学 (2008)
  • Author(s): 矢部俊樹, 海野昌喜, 山下栄樹, 菊池晶裕, 中井正人, 齋藤正男
  • Organizer: 日本生物物理学会東北支部例会
  • Place of Presentation: 仙台
  • Year and Date: 2008-08-30