Study on novel meiosis specific factors for chromosome segregation

• Project/Area Number: 20870011
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: ISHIGURO Kei-ichiro The University of Tokyo, 分子細胞生物学研究所, 助教 (30508114)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: 減数分裂 / 染色体分配 / 生殖細胞 / 動原体 / 細胞分裂 / 染色体

Research Abstract

We have screened meiosis specific centromere factors from mouse germ cells using affinity purification and yeast two hybrid techniques. We identified novel kinetochore/chromosome proteins which are specifically expressed in germ cells. Those factors may play a role in changing kinetochore structure for faithful chromosome segregation in meiosis I where a pair of sister kinetochores are in close proximity for monopolar spindle-microtuble attachment.

Research Products

• [Journal Article] Phosphorylation of H2A by Bub1 Prevents Chromosomal Instability Through Localizing Shugoshin (2010)
  • Author(s): Kawashima SA, Yamagishi Y, Honda T, Ishiguro KI, Watanabe Y
  • Journal Title: Science 327 Pages: 172-177
  • Peer Reviewed
• [Journal Article] Phosphorylation of H2A by Bubl Prevents Chromosomal Instability Through Localizing Shugoshin (2010)
  • Author(s): Kawashima S.A.
  • Journal Title: Science 327 Pages: 172-177
  • Peer Reviewed
• [Presentation] 高等動物における減数分裂特異的な動原体因子の解析 (2010)
  • Author(s): 石黒啓一郎, 南部あや, 渡邊嘉典
  • Organizer: 染色体ワークショップ
  • Place of Presentation: 三島
  • Year and Date: 2010-01-20
• [Presentation] ヒト Bubl はヒストンH2Aのリン酸化を介してシュゴシンの局在位置を規定する (2010)
  • Author(s): 本田貴史, 川島茂裕, 山岸有哉, 石黒啓一郎, 渡邊嘉典
  • Organizer: 染色体ワークショップ
  • Place of Presentation: 三島
  • Year and Date: 2010-01-20
• [Presentation] マウスの減数分裂特異的な動原体タンパク質の解析 (2009)
  • Author(s): 石黒啓一郎
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-13
• [Presentation] マウスの減数分裂特異的な動原体タンパク質の解析 (2009)
  • Author(s): 石黒啓一郎, 南部あや, 渡邊嘉典
  • Organizer: 第32回日本分子生物学会年会ワークショップ
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] マウス減数分裂期における新規染色体因子の同定 (2008)
  • Author(s): 石黒啓一郎, 藤山紗理, 南部あや, 加藤茂明, 渡邊嘉典
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] マウス減数分裂期における新規染色体因子の同定 (2008)
  • Author(s): 石黒啓一郎
  • Organizer: 分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] マウス減数分裂期セントロメア複合体における新規タンパク質の同定 (2008)
  • Author(s): 石黒啓一郎
  • Organizer: 染色体ワークショップ
  • Place of Presentation: 小田原
  • Year and Date: 2008-01-30
• [Book] 分子細胞生物学辞典 (第二版) (2008)
  • Author(s): 石黒啓一郎-分担執筆
  • Publisher: 東京化学同人
• [Book] 分子細胞生物学辞典(第2版) (2008)
  • Author(s): 石黒啓一郎(共同執筆)
  • Total Pages: 1200
  • Publisher: 東京化学同人