| Project/Area Number |
20870022
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
HIRANO Yasuhiro Kyoto University, 生命機能研究科, 特任研究員 (10508641)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
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| Keywords | 核膜 / 1分子解析 / LEMタンパク質 / BAF / リン酸化 / コア領域 / NMR |
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| Research Abstract |
The unique structure termed 'core region' is formed on the center of chromosome at telophase. In this study, I aimed to analyze the core formation mechanism at a single molecular level. To approach this issue, I investigated 1) the 3D-structure of emerin, and 2) a BAF binding site on the chromosomes, those of which were crucial factors to form the core region. By a nuclear magnetic resonance analysis, it was revealed that emerin did not take a solid structure in a solution except the LEM domain, which directly binds to BAF. On the other hand, BAF relatively binds to a transcription-active region at telophase although it binds to a transcription-negative region at interphase. From these evidences, the molecular dynamics of the proteins needed to form the core region are gradually uncovered at a single molecular level.
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