Structural analysis of microtubule motor using peptide array system

• Project/Area Number: 20870033
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Yokohama City University
• Principal Investigator: HAYASHI Ikuko Yokohama City University, 生命ナノシステム科学研究科, 准教授 (80464527)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,198,000 (Direct Cost: ¥2,460,000、Indirect Cost: ¥738,000); Fiscal Year 2009: ¥1,469,000 (Direct Cost: ¥1,130,000、Indirect Cost: ¥339,000); Fiscal Year 2008: ¥1,729,000 (Direct Cost: ¥1,330,000、Indirect Cost: ¥399,000)
• Keywords: 微小管 / 細胞骨格 / x線結晶構造解析 / ペプチドアレイ

Research Abstract

I have established the bacterial over-expression system of the FHA domain region in KIF1C. The oligomerization state of recombinant proteins was examined by gel-filtration and chemical cross-linking analyses. We are now investigating the intra-molecular interaction site using peptide-array system.

Research Products

• [Journal Article] Characterisation of a conserved "threonine clasp" in CAP-Gly domains: role of a functionally critical OH/πinteraction in protein recognition (2008)
  • Author(s): MJ Plevin, I Hayashi, M Ikura
  • Journal Title: J Am Chem Soc 12 Pages: 14918-14919
  • Peer Reviewed
• [Journal Article] 微小管伸長端結合蛋白質の分子制御機構 (2008)
  • Author(s): 林郁子
  • Journal Title: 生化学 6 Pages: 521-530
• [Journal Article] Characterisation of a conserved “threonine clasp"in CAP-Gly domains : role of a functionally critical OH/πinterac tion in protein recognition. (2008)
  • Author(s): MJ Plevin, I Hayashi, M Ikura
  • Journal Title: J Am Chem Soc 12 Pages: 14918-14919
  • Peer Reviewed
• [Journal Article] 微小管伸長端結合蛋白質の分子制御機構 (2008)
  • Author(s): 林 郁子
  • Journal Title: 生化学 6 Pages: 521-530
• [Presentation] Crystal Structure of Microtubule binding protein CLASP2 (2009)
  • Author(s): T Maki, I Hayashi
  • Organizer: Gordon Research Conference
  • Place of Presentation: 米国ニューハンプシャー州
• [Presentation] 微小管伸長端結合蛋白質CLASP2の微小管安定化機構の解析 (2009)
  • Author(s): 真木孝尚, 林郁子
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Crystal Structure of Microtubule binding protein CLASP2 (2009)
  • Author(s): 林郁子, 真木孝尚
  • Organizer: Gordon Research Conference "Motile & Contractile systems"
  • Place of Presentation: アメリカ合衆国ニューハンプシャー州
• [Presentation] 微小管伸長端結合蛋白質CLASP2の微小管安定化機構の解析 (2009)
  • Author(s): 真木孝尚, 林郁子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Remarks] 横浜市立大学大学院バイオエキスパート研究体験シリーズ「イオン濃度を測る・蛍光計測」(平成22年5月29日)にて研究成果の一部を公開
  • URL: http://www.tsurumi.yokohama-cu.ac.jp/biophys/hayashi
• [Remarks]
  • URL: http://www.tsurumi.yokohama-cu.ac.jp/biophys/hayashi
• [Remarks]
  • URL: http://www.tsurumi.yokohama-cu.ac.jp/biophys/hayashi