Elucidation of the molecular recognition mechanism to venomous proteins of a novel small serum protein (SSP) from venomous snake serum.

• Project/Area Number: 20870038
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Fukuoka University
• Principal Investigator: SHIOI Narumi Fukuoka University, 理学部, 助教 (50510187)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: 蛇毒蛋白質 / 毒蛇血清蛋白質 / 生体防御機構 / アポトーシス誘導蛋白質 / 生化学

Research Abstract

Snake venom contains many proteins with several pharmacological functions. On the other hand, endogenous inhibitors to these venomous proteins are present in the snake serum. In the present study, some serum proteins were purified from serum of Habu snake, and the inhibitory effects to the neurotoxin and the hemorrhagic toxin from the own venom were examined. Moreover, one of these proteins strongly inhibited the protein that induces the death of vascular cells. This study also clarified how the serum proteins recognize the venom proteins.

Research Products

• [Journal Article] Terada Snake fetuin: Isolation and structural analysis of new fetuin family proteins from the sera of venomous snakes (2009)
  • Author(s): Narumi Aoki, Masanobu Deshimaru, Kenji Kihara, Shigeyuki
  • Journal Title: Toxicon Vol.54 Pages: 481-490
  • Peer Reviewed
• [Journal Article] CHARACTERISTC DIFFERENCE OF BIOLOGICAL ACTIVITY, BETWEEN ARG-CONTAINING AND LYS-CONTAINING PEPTIDES (2009)
  • Author(s): Yuki Mizoe, Narumi Shioi (Aoki), Shigeyuki Terada, Yoshiki Uchida, Masahide Kuroki, Sannamu Lee
  • Journal Title: Peptide Science Pages: 213-216
  • Peer Reviewed
• [Journal Article] Snake fetuin : Isolation and structural analysis of new fetuin family proteins from the sera of venomous snakes (2009)
  • Author(s): Narumi Aoki
  • Journal Title: Toxicon 54 Pages: 481-490
  • Peer Reviewed
• [Journal Article] CHARACTERISTC DIFFERENCE OF BIOLOGICAL ACTIVITY, BETWEEN ARG-CONTAINING AND LYS-CONTAINING PEPTIDES (2009)
  • Author(s): Yuki Mizoe, Narumi Shioi(Aoki), Shigeyuki Teradal, Yoshiki Uchida, Masahide Kuroki, Sannamu Lee
  • Journal Title: Peptide Science Proceeding 2009 Pages: 213-216
  • Peer Reviewed
• [Journal Article] Terada Accelerated evolution of small serum proteins (SSPs) ; the PSP94 family proteins in a Japanese viper (2008)
  • Author(s): Narumi Aoki, Hisashi Matsuo, Masanobu Deshimaru, Shigeyuki
  • Journal Title: Gene Vol.426 Pages: 7-14
  • Peer Reviewed
• [Journal Article] Accelerated evolution of small serum proteins (SSPs) ; the PSP94 family proteins in a Japanese viper (2008)
  • Author(s): Narumi Aoki
  • Journal Title: Gene 426 Pages: 7-14
  • Peer Reviewed
• [Presentation] CHARACTERISTC DIFFERENCE OF BIOLOGICAL ACTIVITY, BETWEEN ARG-CONTAINING AND LYS-CONTAINING PEPTIDES (2009)
  • Author(s): Yuki Mizoe, Narumi Shioi(Aoki), Shigeyuki Terada, Yoshiki Uchida, Masahide Kuroki, Sannamu Lee
  • Organizer: The 46th Japanese Peptide Symposium
  • Place of Presentation: 福岡(北九州国際会議場)
  • Year and Date: 2009-11-05
• [Presentation] Expression and functional analysis of recombinant SSP-1 (2009)
  • Author(s): 林理恵子、池田真吾、塩井(青木)成留実、寺田成之
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] SSP-2のCRISP及びHSFとの結合領域の解析、A analysis of binding regions of SSP-2 to CRISP and HSF (2009)
  • Author(s): 清村康子、村田順平、塩井(青木)成留実、寺田成之
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] nhibition of apoptosis-inducing activity of Habu venom protein Hvl by SSP-HSF complex (2009)
  • Author(s): 小川瑛輝、水上幸、塩井(青木)成留実、寺田成之
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] SSP-HSF複合体によるハブ毒Hv1のアポトーシス誘導活性の阻害 (2009)
  • Author(s): 小川瑛輝, 水上幸, 塩井(青木)成留実, 寺田成之
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド/国際会議場
  • Year and Date: 2009-10-22
• [Presentation] 大腸菌によるハブSSP-1の発現と機能解析 (2009)
  • Author(s): 林理恵子・池田真吾・塩井(青木)成留実・寺田成之
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド/国際会議場
  • Year and Date: 2009-10-22
• [Presentation] SSP-2のCRISP及びHSFとの結合領域の解析 (2009)
  • Author(s): 清村康子・村田順平・塩井(青木)成留実・寺田成之
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド/国隙会議場
  • Year and Date: 2009-10-22
• [Presentation] ハブゲノム中に存在するL1転移因子様配列の分子進化 (2009)
  • Author(s): 牛尾昴也、田中泰圭、塩井(青木)成留実、弟子丸正伸、寺田成之
  • Organizer: 平成21年度日本生化学会九州支部例会
  • Place of Presentation: 九州大学病院キャンパスコラボステーション
  • Year and Date: 2009-05-17
• [Presentation] ハブ毒Hv1のアポトーシス誘導活性へのSSP-1の影響 (2009)
  • Author(s): 小川瑛輝、塩井(青木)成留実、黒木喜美子、前仲勝実、寺田成之
  • Organizer: 平成21年度日本生化学会九州支部例会
  • Place of Presentation: 九州大学病院キャンパスコラボステーション
  • Year and Date: 2009-05-17
• [Presentation] ハブSSP-1とHSFの結合領域の解析 (2009)
  • Author(s): 池田真吾、林理恵子、塩井(青木)成留実、寺田成之
  • Organizer: 平成21年度日本生化学会九州支部例会
  • Place of Presentation: 九州大学病院キャンパスコラボステーション
  • Year and Date: 2009-05-17
• [Presentation] ハブおよびマムシ血漿中のSSP結合タンパク質の解析 (2009)
  • Author(s): 鬼塚研治、西嶋あゆ美、塩井(青木)成留実、寺田成之
  • Organizer: 平成21年度日本生化学会九州支部例会
  • Place of Presentation: 九州大学病院キャンパスコラボステーション
  • Year and Date: 2009-05-17
• [Presentation] ハブ血清SSP-1の結合タンパク質の同定BMB2008 (2008)
  • Author(s): 青木成留実、楢崎正明、小川瑛輝、寺田成之
  • Organizer: 第31回日本分子生物学会年会.第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] ハブ血清SSP-1の結合タンパク質の同定 (2008)
  • Author(s): ○青木成留実, 楢崎正明, 小川瑛輝, 寺田成之
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2008-12-09