Biochemical study of clock-associated proteins in plants

• Project/Area Number: 20870040
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: NAKAMICHI Norihito The Institute of Physical and Chemical Research, 生産制御研究チーム, 基礎科学特別研究員 (90513440)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: シロイヌナズナ / 生物時計 / 転写因子 / ChIP / 植物 / 概日リズム / PRR / 転写抑制因子 / クロマチン免疫沈降 / 分子生物学 / 低温ストレス / DNAアレイ / インフォマティクス

Research Abstract

I found that PSEUDO-RESPONSE REGULATOR 9 (PRR9), PRR7, and PRR5 act as transcriptional repressors of CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) genes.

Research Products

• [Journal Article] PSEUDO-RESPONSE REGULATORS 9, 7, and 5 are transcriptional repressors in the Arabidopsis circadian clock. (2010)
  • Author(s): Norihito Nakamichi, Takatoshi Kiba, Rossana Henriques, Takeshi Mizuno, Nam-Hai Chua, Hitoshi Sakakibara.
  • Journal Title: The Plant Cell vol.22 Pages: 594-605
  • NAID: 130006991402
  • Peer Reviewed
• [Journal Article] Transcript profiling of an Arabidopsis PSEUDO RESPONSE REGULATOR arrhythmic triple mutant reveals a role for the circadian clock in cold stress response. (2009)
  • Author(s): Norihito Nakamichi, Miyako Kusano, Atsushi Fukushima, Masanori Kita, Shogo Ito, Takafumi Yamashino, Kazuki Saito, Hitoshi Sakakibara, Takeshi Mizuno.
  • Journal Title: Plant Cell Physiol vol.50(3) Pages: 447-462
  • NAID: 10027338110
  • Peer Reviewed
• [Journal Article] Impact of clock-associated Arabidopsis pseudo-response regulators in metabolic coordination. (2009)
  • Author(s): Atsushi Fukushima, Miyako Kusano, Norihito Nakamichi, Makoto Kobayashi, Naomi Hayashi, Hitoshi Sakakibara, Takeshi Mizuno, Kazuki Saito.
  • Journal Title: Proc Natl Acad Sci U S A vol.106(17) Pages: 7251-7256
  • Peer Reviewed
• [Journal Article] Linkage between circadian clock and tricarboxylic acid cycle in Arabidopsis. (2009)
  • Author(s): Norihito Nakamichi, Atsushi Fukushima, Miyako Kusano, Hitoshi Sakakibara, Takeshi Mizuno, Kazuki Saito.
  • Journal Title: Plant Signal Behav. vol.4(7) Pages: 660-662
  • Peer Reviewed
• [Journal Article] Impact of clock-associated Arabidopsis pseudo-response regulators in metabolic coordination (2009)
  • Author(s): Fukushima, et al.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 7251-7256
  • Peer Reviewed
• [Journal Article] Linkage between circadian clock and tricarboxylic acid cycle in Arabidopsis (2009)
  • Author(s): Nakamichi, et al.
  • Journal Title: Plant Signaling & Behavior 4 Pages: 660-662
  • Peer Reviewed
• [Journal Article] Transcript Profiling of an Arabidopsis PSEUDO RESPONSE REGULATOR Arrhythmic Triple Mutant Reveals a Role for the Circadian Clock in Cold Stress Response (2009)
  • Author(s): Nakamichi N, et al.
  • Journal Title: Plant Cell Physiol (印刷中)
  • NAID: 10027338110
  • Peer Reviewed
• [Journal Article] Insight into missing genetic links between two evening-expressed pseudo-response regulator genes TOC1 and PRR5 in the circadian clock-controlled circuitry in Arabidopsis thaliana. (2008)
  • Author(s): Shogo Ito, Yosuke Niwa, Norihito Nakamichi, Hideaki Kawamura, Takafumi Yamashino, Takeshi Mizuno
  • Journal Title: Plant Cell Physiol. vol.49(2) Pages: 201-213
  • NAID: 10027328807
  • Peer Reviewed
• [Journal Article] Involvement of Arabidopsis clock-associated pseudo-response regulators in diurnal oscillations of gene expression in the presence of environmental time cues. (2008)
  • Author(s): Takafumi Yamashino, Shogo Ito, Yosuke Niwa, Atsushi Kunihiro, Norihito Nakamichi, Takeshi Mizuno.
  • Journal Title: Plant Cell Physiol. vol.49(12) Pages: 1839-1850
  • NAID: 10027335869
  • Peer Reviewed
• [Journal Article] A Genetic Study of the Arabidopsis Circadian Clock with Reference to the TIMING OF CAB EXPRESSION 1 (TOC1) Gene (2008)
  • Author(s): Ito S, et al.
  • Journal Title: Plant Cell Physiol (印刷中)
  • Peer Reviewed
• [Journal Article] Involvement of Arabidopsis clock-associated pseudo-response regulators in diurnal oscillations of gene expression in the presence of environmental time cues (2008)
  • Author(s): Yamashino T, et al.
  • Journal Title: Plant Cell Physiol 49 Pages: 1839-1850
  • Peer Reviewed
• [Presentation] シロイヌナズナの疑似レスポンスレギュレーターは生物時計で機能する転写抑制因子である (2010)
  • Author(s): 中道範人、木羽隆敏、Rossana Henriques、水野猛、Nam-Hai Chua、榊原均
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-20
• [Presentation] PSEUDO-RESPONSE REGULATOR 9, 7 and 5 are Transcriptional Repressors of CCA1 and LHY in Arabidopsis Circadian Clock (2009)
  • Author(s): 中道範人、木羽隆敏、水野猛、榊原均
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Pseudo-response regulator 9,7 and 5 are transcriptional repressors of CCA1 and LHY in Arabidopsis circadian clock (2009)
  • Author(s): 中道範人、木羽隆敏、水野猛、榊原均
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] Pseudo-response regulator 9,7 and 5 are repressors of CCA1 and LHY transcription in Arabidopsis circadian clock (2009)
  • Author(s): Nakamichi, et al.
  • Organizer: Plant Biology 2009
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2009-07-26
• [Presentation] PSEUDO-RESPONSE REGULATOR 9, 7 and 5 are Repressors of CCA1 and LHY Transcription in Arabidopsis Circadian Clock (2009)
  • Author(s): Norihito Nakamichi, Takatoshi Kiba, Rossana Henriques, Takeshi Mizuno, Nam-Hai Chua, Hitoshi Sakakibara.
  • Organizer: Plant Biology 2009
  • Place of Presentation: Honolulu
  • Year and Date: 2009-07-23
• [Presentation] シロイヌナズナ時計変異体のアレイ解析 (2009)
  • Author(s): 中道範人、草野都、福島敦史、伊藤照悟、山篠貴史、斉藤和季、水野猛、榊原均
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-18
• [Presentation] 植物時計変異体のDNAアレイ解析 (2008)
  • Author(s): 中道範人、草野都、福島敦史、伊藤照悟、山篠貴史、斉藤和季、水野猛、榊原均
  • Organizer: 第15回時間生物学会学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2008-11-08
• [Presentation] 植物時計変異体のDNAアレイ解析 (2008)
  • Author(s): 中道範人, ら
  • Organizer: 第15回時間生物学会学術大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2008-11-08
• [Remarks] ホームページ
  • URL: http://labs.psc.riken.jp/brt/
• [Remarks] プレスリリース
  • URL: http://www.riken.jp/r-world/info/release/press/2010/100318_2/detail.html
• [Remarks]
  • URL: http://labs.psc.riken.jp/brt/index.html
• [Patent(Industrial Property Rights)] バイオマスが増大し、かつ環境ストレスが向上した形質転換植物およびその作出方法 (2009)
  • Inventor(s): 中道範人、榊原均
  • Industrial Property Rights Holder: 理化学研究所
  • Industrial Property Number: 2009-244595
  • Filing Date: 2009-10-23
  • Acquisition Date: 2009-10-23
• [Patent(Industrial Property Rights)] バイオマスが増大し、かつ環境ストレスが向上した形質転換植物およびその作出方法 (2009)
  • Inventor(s): 中道範人、榊原均
  • Industrial Property Rights Holder: 理化学研究所
  • Filing Date: 2009-12-30
  • Acquisition Date: 2009-12-30
• [Patent(Industrial Property Rights)] バイオマスが増大し、かつ環境ストレス耐性が向上した形質転換植物およびその作出方法 (2009)
  • Inventor(s): 中道範人, 榊原均
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Industrial Property Number: 2009-244595
  • Filing Date: 2009-10-23
• [Patent(Industrial Property Rights)] バイオマスが増大し、かつ環境ストレス耐性が向上した形質転換植物およびその作出方法 (2009)
  • Inventor(s): 中道範人, 榊原均
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Filing Date: 2009-12-30
  • Overseas