Investigation and isolation of unknown fungal groups in subsurface biosphere below the sea-floor.

• Project/Area Number: 20870044
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Biodiversity/Systematics
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: NAGANO Yuriko Japan Agency for Marine-Earth Science and Technology, 海洋・極限環境生物圏領域, ポストドクトラル研究員 (30512917)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,224,000 (Direct Cost: ¥2,480,000、Indirect Cost: ¥744,000); Fiscal Year 2009: ¥1,508,000 (Direct Cost: ¥1,160,000、Indirect Cost: ¥348,000); Fiscal Year 2008: ¥1,716,000 (Direct Cost: ¥1,320,000、Indirect Cost: ¥396,000)
• Keywords: 極限微生物 / 菌類 / 深海環境 / 地下生物圏 / 海洋資源 / 真菌 / 深海 / 地殻 / 極限環境微生物 / 真菌の初期進化 / ツボカビ門菌類 / 生物多様性 / クローン解析

Research Abstract

This study revealed that unknown fungal communities exist in deep subsurface ecosystems with unexpected diversity and novelty. More than 100 fungal species were isolated from deep-sea sediments, deep-sea sponges, shellfishes, sea plants lying on deep-sea floor and other organisms collected in deep-sea environments. All species were identified and screened for antimicrobial agents. As some isolates appear to produce antimicrobial agents, this has great potential for future application research.

Research Products

• [Journal Article] Fungal diversity in deep-sea sediments-the presence of novel fungal groups. (2010)
  • Author(s): Nagano Y, Hatada Y, Nunoura T, Takami H, Miyazaki J, Takai K, Horikoshi K.
  • Journal Title: Fungal Ecology (In press)
  • Peer Reviewed
• [Journal Article] Fungal diversity in deep-sea sediments-the presence of novel fungal groups (2010)
  • Author(s): Yuriko Nagano
  • Journal Title: Fungal Ecology (In press)
  • Peer Reviewed
• [Journal Article] Aquimarina macrocephali sp.nov., isolated from the sediment adjacent to sperm whale carcasses off Kagoshima, Japan (2010)
  • Author(s): Masayuki Miyazaki
  • Journal Title: IJSEM (In press)
  • Peer Reviewed
• [Journal Article] Aquimarina macrocephali sp. nov., isolated from the sediment adjacent to sperm whale carcasses off Kagoshima (2009)
  • Author(s): Miyazaki M, Nagano Y, Fujiwara Y, Hatada Y, Nogi Y.
  • Journal Title: Japan. IJSEM (In press)
  • Peer Reviewed
• [Presentation] 日本における海洋生物の多様性 (2009)
  • Author(s): Fujikura K, Okutani T, Nagahama T, Nogi Y, Lindsay DJ, Yamamoto H, Kitazato H, Nagano Y, Takaki Y, 他40名
  • Organizer: 日本ベントス・プランクトン学会
  • Place of Presentation: Hokkaido, Japan
  • Year and Date: 2009-10-18
• [Presentation] 深海域からのツボカビ様遺伝子の検出と多様性 (2009)
  • Author(s): Nagahama T, Takahashi E, Nagano Y, Miyazaki M, Nogi Y, Horikoshi K.
  • Organizer: 日本菌学会第53回大会
  • Place of Presentation: Tottori, Japan
  • Year and Date: 2009-08-20
• [Presentation] 深海域からのツボカビ様遺伝子の検出と多様性 (2009)
  • Author(s): 長濱統彦
  • Organizer: 日本菌学会第53回大会
  • Place of Presentation: 鳥取大学
  • Year and Date: 2009-08-19
• [Presentation] Discovery of the unknown fungal group that is distributed in global deep-sea environments. (2009)
  • Author(s): Nagano Y, Nagahama T, Hatada Y, Nunoura T, Takami H, Miyazaki J, Takai K, Horikoshi K.
  • Organizer: The 3rd Congress of European Microbiologists
  • Place of Presentation: Gothenburg, Sweden
  • Year and Date: 2009-06-30
• [Presentation] Fungal diversity in deep-sea sediments-Discovery of the unknown fungal group that is distributed in global deep-sea environments- (2009)
  • Author(s): 長野由梨子
  • Organizer: FEMS 2009 The 3rd Congress of European Microbiologists
  • Place of Presentation: Gothenberg, Sweden
  • Year and Date: 2009-06-30
• [Presentation] 深海の微生物に有用酵素を求めて (2009)
  • Author(s): Hatada Y, Nagano Y, Mori K, Konishi M, Ohta Y.
  • Organizer: 第12回マリンバイオテクノロジー学会大会
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2009-05-31
• [Presentation] Industrially relevant enzymes from the deep-sea and the host-vector system for their hyper-production BIT Life Sciences (2009)
  • Author(s): Hatada Y, Nagano Y, Konishi M, Ohta Y.
  • Organizer: 2nd Annual World Congress of Industrial Biotechnology 2009
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-04-06
• [Presentation] Fungal diversity in deep-sea sediments-A whole new world? (2009)
  • Author(s): Nagano Y, Hatada Y, Nagahama T, Nunoura T, Takami H, Horikoshi K.
  • Organizer: The 25th Fungal Genetics Conference
  • Place of Presentation: Pacific Grove, CA, USA
  • Year and Date: 2009-03-18
• [Presentation] Fungal diversity in deep-sea sediments-A whole new world?- (2009)
  • Author(s): 長野由梨子
  • Organizer: 25th Fungal Genetics Conference
  • Place of Presentation: Pacific Grove, CA, U.S.A.
  • Year and Date: 2009-03-18
• [Presentation] 伊豆・小笠原海溝最深部(9, 760m)海底堆積物中における未知真菌群集の調査 (2009)
  • Author(s): Nagano Y, Nagahama T, Hatada Y, Nunoura T, Miyazaki J, Takai K.
  • Organizer: Blue Earth 2009
  • Place of Presentation: okyo, Japan
  • Year and Date: 2009-03-13
• [Presentation] メタン冷湧水域からのBasal fungal lineageの検出と多様性 (2009)
  • Author(s): Nagahama T, Takahashi E, Nagano Y
  • Organizer: Blue Earth 2009
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2009-03-13
• [Presentation] 伊豆・小笠原海溝最深部(9,760m)海底堆積物中における未知真菌群集の調査 (2009)
  • Author(s): 長野由梨子
  • Organizer: Blue Earth 2009
  • Place of Presentation: 立教大学、東京
  • Year and Date: 2009-03-13