Screening of the effective microbial functions for the sustainable resin production

• Project/Area Number: 20880001
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Hirosaki University
• Principal Investigator: SONOKI Tomonori Hirosaki University, 農学生命科学部, 准教授 (20502264)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: テレフタル酸 / p-ヒドロキシ安息香酸 / 相同組換え / サイクロセリン / チロシンアンモニアリアーゼ / 応用微生物 / バイオマス

Research Abstract

This study was focused on the establishment of effective homologous recombination and the identification of essential microbial function for p-hydroxybenzoate (HBA) and terephthalate (TPA) production. The results showed that (i) the effective homologous recombination in Pseudomonas putida JCM6156, (ii) importance of effective expression of tyrosine ammonia lyase, (iii) identification of bacteia involved in 2,5-dihydroxyterephthalate degradation.