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Construction of a genetically-engineered hyper hydrogen-producing strain useful to the future energy-supplying system by hydrogen gas

Research Project

Project/Area Number 20880022
Research Category

Grant-in-Aid for Young Scientists (Start-up)

Allocation TypeSingle-year Grants
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionKyushu Institute of Technology

Principal Investigator

MAEDA Toshinari  Kyushu Institute of Technology, 大学院・生命体工学研究科, 助教 (00470592)

Research Collaborator SANCHEZ-TORRES Viviana  テキサスA&M大学, 化学工学科・博士後期
WOOD Thomas k  テキサスA&M大学, 化学工学科, 教授
Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,029,000 (Direct Cost: ¥2,330,000、Indirect Cost: ¥699,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,729,000 (Direct Cost: ¥1,330,000、Indirect Cost: ¥399,000)
Keywords水素ガス / バイオテクノロジー / 微生物 / 生体機能利用 / 遺伝子工学 / 代謝工学 / タンパク質工学 / 水素
Research Abstract

It would be certainly required to develop a new energy-supplying system such as hydrogen energy in place of petroleum energy. This study attempted to enhance bacterial hydrogen production as much as possible by genetic engineering which allows to activate a metabolic pathway and to enhance the enzyme catalytic function related to hydrogen production. In addition, some interesting results obtained by random DNA mutation approach and DNA microarray approach would hold great promise for further improving bacterial hydrogen production.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (26 results)

All 2009 2008 Other

All Journal Article (15 results) (of which Peer Reviewed: 14 results) Presentation (8 results) Remarks (3 results)

  • [Journal Article] 大腸菌の代謝改変およびタンパク質改変による水素ガスの高度生産化2009

    • Author(s)
      前田憲成、尾川博昭
    • Journal Title

      Journal of Environmental Biotechnology Vol.9、No.2

      Pages: 69-74

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein engineering of the transcriptional activator FhlA to enhance Escherichia coli hydrogen production2009

    • Author(s)
      SANCHEZ-TORRES V., MAEDA T., WOOD T.
    • Journal Title

      Applied and Environmental Microbiology Vol.75,No.17

      Pages: 5639-5646

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 大腸菌の代謝改変およびタンパク質改変による水素ガスの高度生産化2009

    • Author(s)
      前田憲成, 尾川博昭
    • Journal Title

      Journal of Environmental Biotechnology 9

      Pages: 69-74

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protein engineering of the transcriptional activator FhIA to enhance Escherichia coli hydrogen production2009

    • Author(s)
      SANCHEZ-TORRES V., MAEDAT., WOOD T.
    • Journal Title

      Applied and Environmental Microbiology 75

      Pages: 5639-5646

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reconfiguring the quorum-sensing regulator SdiA of Escherichia coli to control biofilm formation via indole and N-acylhomoserine lactones2009

    • Author(s)
      LEE J., MAEDA T., HONG S.H., WOOD T.K.
    • Journal Title

      Applied and Environmental Microbiology 75

      Pages: 1703-1716

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced production of lactic acid with reducing excess sludge by lactate fermentation2009

    • Author(s)
      MAEDA T., YOSHIMURA T., SHIMAZU T., SHIRAI Y., OGAWA H.I.
    • Journal Title

      Journal of Hazardous Materials 168

      Pages: 656-663

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formate detection by potassium permanganate for enhanced hydrogen production in Escherichia coli2008

    • Author(s)
      Maeda T., Wood T.K.
    • Journal Title

      International Journal of Hydrogen Energy Vol.33,No.9

      Pages: 2409-2412

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein engineering of hydrogenase 3 to enhance hydrogen production2008

    • Author(s)
      Maeda T., Sanchez-Torres V., Wood T.K. Wood
    • Journal Title

      Applied Microbiology and Biotechnology Vol.79,No.1

      Pages: 77-86

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metabolically-engineered bacteria for producing hydrogen via fermentation2008

    • Author(s)
      Vardar-Schara G., Maeda T., Wood T.K.
    • Journal Title

      Microbial Biotechnology Vol.1,No.2

      Pages: 107-125

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metabolic engineering to enhance bacterial hydrogen production2008

    • Author(s)
      Maeda T., Sanchez-Torres V., Wood T.K.
    • Journal Title

      Microbial Biotechnology Vol.1,No.1

      Pages: 33-39

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein engineering of hydrogenase 3 to enhance hydrogen production2008

    • Author(s)
      MAEDA Toshinari
    • Journal Title

      Applied Microbiology and Biotechnology 79

      Pages: 77-86

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formate detection by potassium permanganate for enhanced hydrogen production in Escherichia coli2008

    • Author(s)
      MAEDA Toshinari
    • Journal Title

      Internal Journal of Hydrogen Energy 33

      Pages: 2409-2412

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolically-engineered bacteria for producing hydrogen viafermentation2008

    • Author(s)
      VARDAR-SCHALA Gonul
    • Journal Title

      Microbial Biotechnology 1

      Pages: 107-125

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolic engineering to enhance bacterial hydrogen production2008

    • Author(s)
      MAEDA Toshinari
    • Journal Title

      Microbial Biotechnology 1

      Pages: 30-39

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolic Engineering to Enhance Bioenergy Production

    • Author(s)
      MAEDA T.
    • Journal Title

      The 9th International Symposium on Clean Technology and Green Energy

    • Related Report
      2009 Final Research Report
  • [Presentation] Metabolic Engineering to Enhance Bioenergy Production2009

    • Author(s)
      MAEDA T.
    • Organizer
      The 9th International Symposium on Clean Technology and Green Energy
    • Place of Presentation
      Yeungnam University, Gyeongbuk, Korea
    • Year and Date
      2009-11-13
    • Related Report
      2009 Final Research Report
  • [Presentation] Metabolic Engineering to Enhance Bioenergy Production2009

    • Author(s)
      MAEDA T.
    • Organizer
      The 9th International Symposium on Clean Tech nology and Green Energy
    • Place of Presentation
      Yeungnam University, Gyeongbuk, Korea
    • Year and Date
      2009-11-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] 大腸菌の代謝改変およびタンパク質改変による水素ガスの高度生産化2009

    • Author(s)
      前田憲成
    • Organizer
      第37回シンポジウム「バイオエネルギー生産の最前線」, 環境バイオテクノロジー学会
    • Place of Presentation
      東京大学
    • Year and Date
      2009-06-24
    • Related Report
      2009 Final Research Report
  • [Presentation] 大腸菌の代謝改変およびタンパク質改変による水素ガスの高度生産化2009

    • Author(s)
      前田憲成
    • Organizer
      第37回シンポジウム「バイオエネルギー生産の最前線」,環境バイオテクノロジー学会
    • Place of Presentation
      東京大学
    • Year and Date
      2009-06-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Protein Engineering of Hydrogenase 3 to Enhance Hydrogen Production2008

    • Author(s)
      前田憲成、尾川博昭、Wood T.K.
    • Organizer
      第31回日本分子生物学会・第81回日本生化学大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2009 Final Research Report
  • [Presentation] Protein engineering of hydrogenase 3 to enhance hydrogen production2008

    • Author(s)
      前田憲成
    • Organizer
      第31回日本分子生物学会・第81回日本生化学大会合同大会BMB2008
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] シアノバクテリアの水素生成酵素による大腸菌の水素消費活性の阻害2008

    • Author(s)
      前田憲成、尾川博昭、Wood Thomas K.
    • Organizer
      第60回日本生物工学会大会
    • Place of Presentation
      東北学院大学
    • Year and Date
      2008-08-27
    • Related Report
      2009 Final Research Report
  • [Presentation] シアノバクテリアの水素生成酵素による大腸菌の水素消費活性の阻害2008

    • Author(s)
      前田憲成
    • Organizer
      第60回日本生物工学会大会
    • Place of Presentation
      東北学院大学土樋キャンパス
    • Year and Date
      2008-08-27
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://horyu.jimut.kyutech.ac.jp/kit/AnnualReport2010.nsf

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://horyu.jimut.kyutech.ac.jp/kyouin/index.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://horyu.jimut.kyutech.ac.jp/kyouin/index.html

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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