| Project/Area Number |
20880031
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Okayama University of Science |
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Principal Investigator |
HARA Hirofumi Okayama University of Science, 工学部, 講師 (80511071)
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| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
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| Keywords | 放線菌 / 環境汚染物質 / ChIP-chip解析 / 二成分制御系 / 転写制御因子 / 環境汚染物質分解 / ロドコッカス属細菌 / DNAマイクロアレイ / 網羅的解析 |
|---|
| Research Abstract |
Rhodococcus jostii RHA1 was grown on a broad range of aromatic compounds and vigorously degrades polychlorinated biphenyls (PCBs). Two-component signal transduction system, BphT1T2, is necessary for activation of biphenyl degradation genes in R. jostii RHA1. In this study, we performed global chromatin immunopercipitation-on-chip (ChIP-chip) analysis to identify novel BphT1 binding region in R. jostii RHA1. As a result, we identified 9 ORFs as direct target of BphS1T1, which including IclR type transcriptional regulator, possible endonuclease, and some hypothetical proteins. This result indicated that BphS1T1 is a master regulator of biphenyl degradation, and directly activated to transcriptional regulator and endonibonuclease to regulate expression level of many ORFs in RHA1.
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