| Project/Area Number |
20880040
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | National Agricultural Research Organization |
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Principal Investigator |
HATTA Takeshi National Agricultural Research Organization, 動物衛生研究所, 研究員 (00455304)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥3,133,000 (Direct Cost: ¥2,410,000、Indirect Cost: ¥723,000)
Fiscal Year 2009: ¥1,456,000 (Direct Cost: ¥1,120,000、Indirect Cost: ¥336,000)
Fiscal Year 2008: ¥1,677,000 (Direct Cost: ¥1,290,000、Indirect Cost: ¥387,000)
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| Keywords | マダニ / アミノペプチダーゼ / バベシア原虫 / マダニ媒介感染症 |
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| Research Abstract |
Tick is well known biological vector of zoonotic infectious diseases such as Lyme disease but the tick molecular function regarding pathogen transmission is poorly understood. A terminal enzyme of host blood digestion in tick midgut was an aminopeptidase, HlLAP which was shown to be involved in the regulatory effect on babesial migration in tick by inhibiting its invasion. Based on the cellular biological study, we observed that HlLAP gene silencing by RNA interference induced the immature generation of peritrophic membrane (PM) in midgut. PM is the mechanical barrier of midgut epithelium against bacterial or xenobiotic substances in host blood meal. Therefore, it was assumed that HlLAP which was responsible for the generation of PM disruption of which inhibited indirectly an invasion of Babesia parasite into the midgut epithelium.
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