Development of glycopeptide array aiming for diagnosis of infectious diseases
| Project/Area Number |
20890007
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Hokkaido University |
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Principal Investigator |
KATO Kentaro Hokkaido University, 大学院・先端生命科学研究院, 博士研究員 (50508885)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,224,000 (Direct Cost: ¥2,480,000、Indirect Cost: ¥744,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,664,000 (Direct Cost: ¥1,280,000、Indirect Cost: ¥384,000)
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| Keywords | 糖ペプチドアレイ / レクチン / 感染症 |
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| Research Abstract |
MUC2 mucin (glyco)peptides with fifty-six patterns of N-acetylgalactosamine (GalNAc) residue attachment were synthesized and immobilized on the plate. Binding affinities of VVA-B4, a plant lectin, toward GalNAc attached MUC2 peptides were measured using ELISA. The results suggested that the affinity was defined by the sites of GalNAc attachment on the peptides. The plate with GalNAc attached MUC2 peptides was successfully developed and this made possible for measuring the affinities of parasite lectins toward GalNAc attached MUC2 peptides.
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Report
(3 results)
Research Products
(2 results)
