Development of glycopeptide array aiming for diagnosis of infectious diseases

Research Project

Project/Area Number	20890007
Research Category	Grant-in-Aid for Young Scientists (Start-up)
Allocation Type	Single-year Grants
Research Field	Laboratory medicine
Research Institution	Hokkaido University
Principal Investigator	KATO Kentaro Hokkaido University, 大学院・先端生命科学研究院, 博士研究員 (50508885)
Project Period (FY)	2008 – 2009
Project Status	Completed (Fiscal Year 2009)
Budget Amount *help	¥3,224,000 (Direct Cost: ¥2,480,000、Indirect Cost: ¥744,000) Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2008: ¥1,664,000 (Direct Cost: ¥1,280,000、Indirect Cost: ¥384,000)
Keywords	糖ペプチドアレイ / レクチン / 感染症
Research Abstract	MUC2 mucin (glyco)peptides with fifty-six patterns of N-acetylgalactosamine (GalNAc) residue attachment were synthesized and immobilized on the plate. Binding affinities of VVA-B4, a plant lectin, toward GalNAc attached MUC2 peptides were measured using ELISA. The results suggested that the affinity was defined by the sites of GalNAc attachment on the peptides. The plate with GalNAc attached MUC2 peptides was successfully developed and this made possible for measuring the affinities of parasite lectins toward GalNAc attached MUC2 peptides.