Analysis of nano-drug delivery system in tumor with low vascular permeability with permeability-enhancing agents.

• Project/Area Number: 20890018
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: General surgery
• Research Institution: Tohoku University
• Principal Investigator: KAWAI Masaaki Tohoku University, 大学院・医学系研究科, 助教 (80513530)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: DDS / 新生血管 / EPR効果 / 量子ドット / 血管内皮細胞増殖因子 / 虚血性腫瘍 / Quantum Dot / マウス片側肢虚血モデル / 血管透過性亢進剤 / in vivoイメージング

Research Abstract

We clarified appropriate sizes of nanoparticles to use enhanced permeability and retention effects by the single molecular imaging method in vivo. The dynamics of the particles depends on the place in the tumor. We made vascular endothelial growth factor (VEGF)-quantum dot (Qdot) complex. VEGF-Qdot complex attaches vascular endothelial growth factor receptor (VEGFR) expressing tumor cell in vitro higher than platlet-derived growth factor (PDGF)-Qdot and Qdot only. We confirmed reduced blood flow of the mouse ischemic limb model we made by the laser Doppler apparatus.

Research Products

• [Journal Article] Dynamics of different sized solid state nanocrystals as tracers for a drug delivery system in the interstitium of a human tumor xenograft (2009)
  • Author(s): Kawai M, Higuchi H, Takeda M, Kobayashi Y, Ohuchi N.
  • Journal Title: Breast Cancer Research
  • Peer Reviewed
• [Journal Article] Dynamics of different sized solid state nanocrystals as tracers for a drug delivery system in the interstitium of a human tumor xenograft. (2009)
  • Author(s): Kawai M, Higuchi H, Takeda M, Kobayashi Y, Ohuchi N
  • Journal Title: Breast Cancer Research 11
  • Peer Reviewed
• [Presentation] 分子イメージングIn vivoナノイメージングで解き明かすがん転移とDDSの仕組み (2009)
  • Author(s): 権田幸祐、渡邉朋信、河合賢朗、武田元博、樋口秀男、大内憲明
  • Organizer: 第25回日本DDS学会学術集会
  • Place of Presentation: 東京ドームホテル
  • Year and Date: 2009-07-03
• [Presentation] ナノ粒子をトレーサーとした鏡視下手術の基礎的検討 (2009)
  • Author(s): 日景允, 河合賢朗
  • Organizer: ナノ学会第7回大会
  • Place of Presentation: 東京大学本郷キャンパス (浅野地区) 武田ホール
  • Year and Date: 2009-05-10
• [Presentation] ナノ粒子をトレーサーとした鏡視下手術の基礎的検討 (2009)
  • Author(s): 日景允, 武田元博, 亀井尚, 権田幸祐, 小林正樹, 熊坂増高, 中野徹, 櫻井遊, 河合賢朗, 叢莉蔓, 濱中洋平, 濱田庸, 里見進, 大内憲明
  • Organizer: ナノ学会第7回大会
  • Place of Presentation: 東京大学本郷キャンパス(浅野地区)武田ホール
  • Year and Date: 2009-05-10
• [Presentation] Novel bio-imaging by functional nano-particle (2009)
  • Author(s): Ohuchi N, Kawai M.
  • Organizer: 9th International Symposium of Tohoku University Global COE Program
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2009-03-24
• [Presentation] Novel bio-imaging by functional nano-particle (2009)
  • Author(s): Ohuchi N, Takada M, Gonda K, Kawai M, Cong L, Sakurai Y
  • Organizer: 9^<th> International Symposium of Tohoku University Global COE Program
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2009-03-24
• [Presentation] Bioimaging and dynamics of functional nano-particles (2009)
  • Author(s): Takeda M, Kawai M
  • Organizer: The 2nd International Symposium on Nanomedicine, Asian Core Symposium-Nano and Biomedical Molecular Science-
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2009-02-04
• [Presentation] Basic examination of the fluorescent endoscopic surgery using nanoparticle tracer (2009)
  • Author(s): Hikage M, Kawai M.
  • Organizer: The 2nd International Symposium on Nanomedicine, Asian Core Symposium-Nano and Biomedical Molecular Science-
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2009-02-04
• [Presentation] Bioimaging and dynamics of functional nano-particles (2009)
  • Author(s): Takeda M, Gonda K, Kawai M, Sakurai Y, Cong L, Higuchi H, Ohuchi N
  • Organizer: The 2^<nd> International Symposium on Nanomedicine, Asian Core Symposium-Nano anc Biomedical Molecular Science-
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2009-02-04
• [Presentation] Basic examination of the fluorescent endoscopic surgery using nanoparticle tracer (2009)
  • Author(s): Hikage M, Takeda M, Kamei T, Kobayashi M, Gonda K, Nakano T, Sakurai Y, Kawai M, Liman C, Hamanaka Y, Kumasaka M, Satomi S, Ohuchi N
  • Organizer: The 2^<nd> International Symposium on Nanomedicine, Asian Core Symposium-Nano and Biomedical Molecular Science-
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2009-02-04
• [Presentation] 分子イメージング In vivo ナノイメージングで解き明かすがん転移とDDSの仕組み (2009)
  • Author(s): 権田幸祐, 河合賢朗
  • Organizer: 第25回日本DDS学会学術集会
  • Place of Presentation: 東京ドームホテル
• [Presentation] Bio-imaging by functional nano-particles of nano to macro scale (2008)
  • Author(s): Takeda M, Kawai M.
  • Organizer: The 13th Internatonal Conferencenon Biomedical Engineering
  • Place of Presentation: National University of Singapore, Singapore
  • Year and Date: 2008-12-04
• [Presentation] The feature of the interstitial nano drug delivery system with fluorescent nanocrystals of different sizes in the human tumor xenograft in mice (2008)
  • Author(s): Kawai M.
  • Organizer: The 13th Internatonal Conferencenon Biomedical Engineering
  • Place of Presentation: National University of Singapore, Singapore
  • Year and Date: 2008-12-04
• [Presentation] 機能性ナノ粒子による生体イメージングの臨床検査・外科への応用 (2008)
  • Author(s): 武田元博, 河合賢朗
  • Organizer: 第55回日本臨床検査医学会学術集会、シンポジウム10 : 医学領域におけるナノ粒子展開をめぐる話題「機能性ナノ粒子による生体イメージングの臨床検査への応用」
  • Place of Presentation: 名古屋
  • Year and Date: 2008-11-27
• [Presentation] Imaging of cancer metastasis in living tumor with quantum dot (2008)
  • Author(s): Gonda K, Kawai M.
  • Organizer: The 7th International Symposium on Nano-Biomedical Engineering
  • Place of Presentation: National Cheng Kung University, Tainan
  • Year and Date: 2008-10-24
• [Presentation] Imaging of cancer metastasis in living tumor with quantum dots (2008)
  • Author(s): Gonda K, Takeda M, Kawai M, Sakurai Y, Higuchi H, Ohuchi, N
  • Organizer: The 7th International Symposium on Nano-Biomedical Engineering
  • Place of Presentation: National Cheng Kung University, Tainan
  • Year and Date: 2008-10-24
• [Presentation] 蛍光ナノ粒子による腫瘍間質ドラッグデリバリーシステムの生体内一分子イメージング (2008)
  • Author(s): 河合賢朗
  • Organizer: 第16回 日本乳癌学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-27
• [Presentation] 蛍光ナノ粒子による腫瘍間質ドラッグデリバリーシステムの生体内-分子イメージング (2008)
  • Author(s): 河合賢朗
  • Organizer: 第16回日本乳癌学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-27
• [Presentation] Interstitial Nano-particle Tracking and Analysis of the Movements in the Human Tumor Xenografts in Mice (2008)
  • Author(s): Kawai M.
  • Organizer: The 26th Congress of the International Association for Breast Cancer Research
  • Place of Presentation: 岡山県倉敷市
  • Year and Date: 2008-09-24
• [Presentation] Interstitial Nano-particle Tracking and Analysis of the Movements in the Human Tumor Xenografts in Mice (2008)
  • Author(s): Kawai M
  • Organizer: The 26^<th> Congress of the International Association for Breast Cancer Research
  • Place of Presentation: 岡山県倉敷市
  • Year and Date: 2008-09-24
• [Presentation] 粒径の異なるナノ粒子による腫瘍間質ドラッグデリバリーシステムの解析 (2008)
  • Author(s): 河合賢朗
  • Organizer: 第18回乳癌基礎研究会
  • Place of Presentation: 福島
  • Year and Date: 2008-07-15
• [Presentation] 腫瘍間質におけるナノスケールでの分子挙動解析によるDDSの解明 (2008)
  • Author(s): 河合賢朗、樋口秀男、武田元博、石田孝宣、鈴木昭彦、甘利正和、宇佐美伸、桜井遊、渡部剛、大内憲明
  • Organizer: 第108回日本外科学会定期学術集会
  • Place of Presentation: 長崎
  • Year and Date: 2008-05-15
• [Presentation] 蛍光ナノ粒子を用いた担がんマウスにおける腫瘍間質ナノドラッグデリバリーシステムの解析 (2008)
  • Author(s): 河合賢朗
  • Organizer: ナノ学会第6回大会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-07
• [Book] 外科治療. 外科医に必要ながん化学療法の知識B. がん化学療法の実際7. 乳癌「乳癌の集学的治療と化学療法」 (2008)
  • Author(s): 河合賢朗
• [Book] 臨床病理. 医学領域におけるナノ粒子展開をめぐる話題機能性ナノ粒子による生体イメージングの臨床検査への応用 (2008)
  • Author(s): 武田元博, 河合賢朗
  • Total Pages: 60
• [Book] 外科医に必要ながん化学療法の知識B. がん化学療法の実際7. 乳癌「乳癌の集学的治療と化学療法」 (2008)
  • Author(s): 河合賢朗、武田元博、大内憲明一
  • Publisher: 外科治療