The immunoregulation of Notch signaling in graft versus host disease.

• Project/Area Number: 20890031
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: University of Tsukuba
• Principal Investigator: NISHIKII Hidekazu University of Tsukuba, 附属病院, 病院講師 (30512834)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: Notchシグナル / 移植片対宿主病 / 造血幹細胞移植

Research Abstract

To clalify how Notch signal works on the immunoregulation system in the graft versus host disease (GVHD), we established the mouse-GVHD model using bone marrow transplant. We also established the purification system of hematopoietic progenitor cells derived from bone marrow and T cells derived from spleen using FACS sorting and MACS. Then, we modified T cell signaling by high-titered retrovirus system.