Anti-tumor effect of connexin gene for cancer therapy

• Project/Area Number: 20890036
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Chiba University
• Principal Investigator: SATO Hiromi Chiba University, 大学院・薬学研究院, 助教 (30506887)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: がん / 薬学 / エピジェネティック / 薬剤感受性 / 併用効果 / コネキシン遺伝子 / 脱メチル化薬 / 遺伝子 / 癌

Research Abstract

We have investigated the anti-tumor effect of connexin (Cx) gene, component of intercellular gap junction. Finally 5-aza-2'-deoxyxytidine could induce Cx32 expression in renal cell carcinoma, which exerted not only anti-proliferative effect but also improvement of chemotherapeutic cytotoxicity. On the other hands, Cx43 was found to enhance cisplatin cytotoxicity against mesothelioma. Above all, Cx could be useful for treatment of refractory cancers.

Research Products

• [Journal Article] Enhanced effect of connexin 43 on cisplatin-induced cytotoxicity in mesothelioma cells (2009)
  • Author(s): Sato H, Iwata H, Takano Y, Yamada R, Okuzawa H, Nagashima Y, Yamaura K, Ueno K, Yano T.
  • Journal Title: Journal of Pharmacological Sciences 110 Pages: 466-475
  • NAID: 10025738277
  • Peer Reviewed
• [Journal Article] Improvement of sensitivity to vinblastine by restoration of connexin 32 gene in renal cell carcinoma cells (2009)
  • Author(s): Takano Y, Iwata H, Sato H, Okuzawa H, Yamada R, Nagashima Y, Yamaura K, Ueno K, Yano T.
  • Journal Title: Proc AACR 50 Pages: 713-713
  • Peer Reviewed
• [Journal Article] Comparative study of histamine H4 receptor expression in human dermal fibroblasts (2009)
  • Author(s): Ikawa Y, Shiba K, Ohki E, Mutoh N, Suzuki M, Sato H, Ueno K.
  • Journal Title: The Journal of Toxicological Sciences 33 Pages: 503-508
  • NAID: 110006950531
  • Peer Reviewed
• [Journal Article] Expression of histamine H4 receptor in human epidermal tissues and attenuation of experimental pruritus using H4 receptor agonist (2009)
  • Author(s): Yamaura K, Oda M, Suwa E, Suzuki M, Sato H, Ueno K.
  • Journal Title: The Journal of Toxicological Sciences 34 Pages: 427-431
  • Peer Reviewed
• [Journal Article] Enhanced effect of connexin 43 on cisplatin-induced cytotoxicity in mesothelioma cell. (2009)
  • Author(s): Sato H, Iwata H, Takano Y, Yamada R, Okuzawa H, Nagashima Y, Yamaura K, Ueno K, Yano T
  • Journal Title: Journal of Pharmacological Sciences 110 Pages: 466-475
  • NAID: 10025738277
  • Peer Reviewed
• [Journal Article] Improvement of sensitivity to vinblastine by restoration of connexin 32 gene in renal cell carcinoma cells. (2009)
  • Author(s): Takano Y, Iwata H, Sato H, Okuzawa H, Yamada R, Nagashima Y, Yamaura K, Ueno K, Yano T
  • Journal Title: Proc AACR 50 Pages: 713-713
  • Peer Reviewed
• [Journal Article] Comparative study of histamine H4 receptor expression in human dermal fibroblasts. (2009)
  • Author(s): Ikawa Y, Shiba K, Ohki E, Mutoh N, Suzuki M, Sato H, Ueno K.
  • Journal Title: The Journal of Toxicological Sciences 33 Pages: 503-508
  • NAID: 110006950531
  • Peer Reviewed
• [Journal Article] Expression of histamine H4 receptor in human epidermal tissues and attenuation of experimental pruritus H4 receptor agonist. (2009)
  • Author(s): Yamaura K, Oda M, Suwa E, Suzuki M, Sato H, Ueno K.
  • Journal Title: The Journal of Toxicological Sciences 34 Pages: 427-431
  • Peer Reviewed
• [Journal Article] The inhibitory effect of connexin 32 gene on metastasis in renal cell carcinoma (2008)
  • Author(s): Sato H, Hagiwara H, Senba H, Fukumoto K, Nagashima Y, Yamasaki H, Ueno K, Yano T.
  • Journal Title: Molecular Carcinogenesis 47 Pages: 403-409
  • Peer Reviewed
• [Journal Article] The inhibitory effect of connexin 32 gene on metastasis in renal cell carcinoma. (2008)
  • Author(s): Sato H, Hagiwara H, SenbaH, Fukumoto K, Nagashima Y, Yamasaki H, Ueno K and Yano T.
  • Journal Title: Molecular Carcinogenesis 47 Pages: 403-409
  • Peer Reviewed
• [Presentation] 脱メチル化薬による腎細胞癌のビンブラスチン感受性増強作用 (2010)
  • Author(s): 岩田紘樹, 佐藤洋美, 山田遼太, 一之宮紗紀, 柳原碧, 山浦克典, 矢野友啓, 上野光一
  • Organizer: 第130回日本薬学会年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] ミカン科植物由来成分全合成中間体の癌細胞増殖抑制作用の検討 (2010)
  • Author(s): 柳原碧, 山田遼太, 佐藤洋美, 岩田紘樹, 熊本卓哉, 一之宮紗紀, 山浦克典, 矢野友啓, 石川勉, 上野光一
  • Organizer: 第130回日本薬学会年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] 悪性中皮腫細胞におけるγ-トコトリエノール (γ-T3) とスタチンの併用効果 (2010)
  • Author(s): 一之宮紗紀, 佐藤洋美, 中島啓介, 岩田紘樹, 山田遼太, 柳原碧, 山浦克典, 矢野友啓, 上野光一
  • Organizer: 第130回日本薬学会年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] 悪性中皮腫細胞におけるγ-トコトリエノール(γ-T3)とスタチンの併用効果 (2010)
  • Author(s): 一之宮紗紀、佐藤洋美、中島啓介、岩田紘樹、山田遼太、柳原碧、山浦克典、矢野友啓、上野光一
  • Organizer: 第130回日本薬学会年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] Anti-tumor activities of p-quinone monooximes obtained from the synthetic pathway of natural product, macarpine (2010)
  • Author(s): Ryota Yamada, Hiromi Sato, Hiroki Iwata, Midori Yanagihara, Saki Ichinomiya, Tomohiro Yano, Katsunori Yamaura, Koichi Ueno
  • Organizer: 第83回日本薬理学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-03-17
• [Presentation] The tumor suppressive effect of connexin gene (2009)
  • Author(s): Hiromi Sato
  • Organizer: JSPS拠点交流事業セミナー (R-1)
  • Place of Presentation: Chulalongkorn University, Bangkok, Thailand
  • Year and Date: 2009-12-01
• [Presentation] The tumor suppressive effect of connexin gene (2009)
  • Author(s): Hiromi Sato
  • Organizer: JSPS拠点交流事業セミナー(R-1)
  • Place of Presentation: Chulalongkorn University, Bangkok, Thailand
  • Year and Date: 2009-12-01
• [Presentation] コネキシン43導入による悪性中皮腫細胞の薬物感受性の改善作用について (2009)
  • Author(s): 山田遼太, 佐藤洋美, 岩田紘樹, 一之宮紗紀, 柳原碧, 山浦克典, 矢野友啓, 上野光一
  • Organizer: 第121回 日本薬理学会関東部会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-10
• [Presentation] Anti-tumor activities of p-quinone monooximes obtained from the synthetic pathway of natural product, macarpine (2009)
  • Author(s): Hiroki Iwata, Hiromi Sato, Tomohiro Yano Koichi Ueno
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-03
• [Presentation] Combination effect of a HDAC inhibitor and sunitinib against renal cell carcinoma (2009)
  • Author(s): Hiromi Sato, Hiroki Iwata, Tomohiro Yano Koichi Ueno
  • Organizer: 第68回 日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-03
• [Presentation] Anti-tumor activities of p-quinone monooximes obtained from the synthetic pathway of natural product, macarpine (2009)
  • Author(s): Hiroki Iwata, Hiromi Sato, Tomohiro Yano, Koichi Ueno
  • Organizer: 第68回 日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-03
• [Presentation] Induction of connexin 32 by 5-Aza-2'-deoxycytidine can enhance vinblastine-induced cytotoxicity in renal cell carcinoma (2009)
  • Author(s): Yasuyuki Takano, Hiroki Iwata, Hiromi Sato, Tomohiro Yano Koichi Ueno
  • Organizer: 第68回 日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-01
• [Presentation] Improvement of sensitivity to vinblastine by restoration of connexin 32 gene in renal cell carcinoma cells (2009)
  • Author(s): Yasuyuki Takano, Hiroki Iwata, Hiromi Sato, Hiroko Okuzawa, Ryota Yamada, Yoji Nagashima, Katsunori Yamaura, Koichi Ueno, Tomohiro Yano
  • Organizer: 米国癌学会議 (AACR) 100^<th> Annual Meeting
  • Place of Presentation: Denver, CO, USA
  • Year and Date: 2009-04-19
• [Presentation] Improvement of sensitivity to vinblastine by restoration of connexin 32 gene in renal cell carcinoma cells (2009)
  • Author(s): Yasuyuki Takano, Hiroki Iwata, Hiromi Sato, Hiroko Okuzawa, Ryota Yamada, Yoji Nagashima, Katsunori Yamaura, Koichi Ueno, Tomohiro Yano
  • Organizer: 米国癌学会(AACR)100^<th> Annual meeting
  • Place of Presentation: Denver, CO, USA
  • Year and Date: 2009-04-19
• [Presentation] Improvement of chemosensitivity by restoration of connexin 32 gene (2009)
  • Author(s): Hiromi Sato, Yasuyuki Takano, Hiroki Iwata, Hiroko Okuzawa, Ryota Yamada, Katsunori Yamaura, Koichi Ueno, Tomohiro Yano
  • Organizer: 第82回 日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-18
• [Presentation] Improvement of chemosensitivity by restoration of connexin 32 gene. (2009)
  • Author(s): Hiromi Sato, Yasuyuki Takano, Hiroki Iwata, Hiroko Okuzawa, Ryota Yamada, Katsunori Yamaura, Koichi Ueno, Tomohiro Yano
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-03-18
• [Presentation] コネキシン遺伝子の薬物感受性改善作用に関する研究 (2009)
  • Author(s): 佐藤洋美, 岩田紘樹, 高野泰幸, 奥澤紘子, 山田遼太, 山浦克典, 上野光一, 矢野友啓
  • Organizer: 第19回 細胞間ジャンクション研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-01-17
• [Presentation] コネキシン遺伝子の薬物感受性改善作用に関する研究 (2009)
  • Author(s): 佐藤洋美、岩田紘樹、高野泰幸、奥澤紘子、山田遼太、山浦克典、上野光一、矢野友啓
  • Organizer: 第19回細胞間ジャンクション研究会
  • Place of Presentation: 大手町サンケイブラザ
  • Year and Date: 2009-01-17
• [Presentation] The tumor suppressive effect of connexin 32 gene (2008)
  • Author(s): Hiromi Sato, Tomohiro Yano, Hiroki Iwata, Yasuyuki Takano, Hiroko Okuzawa, Ryota Yamada, Hiromi Hagiwara, Koichi Ueno
  • Organizer: 第2回次世代を担う若手医療薬科学シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2008-12-20
• [Presentation] The tumor suppressive effect of connexin 32 gene. (2008)
  • Author(s): Hiromi Sato, Tomohiro Yano, Hiroki Iwata, Yasuyuki Takano, Hiroko Okuzawa, Ryota Yamada, Hiromi Hagiwara, Koichi Ueno
  • Organizer: 第2回次世代を担う若手医療薬科学シンポジウム
  • Place of Presentation: 京都大学
  • Year and Date: 2008-12-20
• [Presentation] Connexin 43 potentiates cisplatin-induced citotoxicity in malignant mesothelioma cells (2008)
  • Author(s): Hiromi Sato, Tomohiro Yano, Yasuyuki Takano, Hiroki Iwata, Koichi Ueno
  • Organizer: 第67回 日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] Connexin 43potentiates cisplatin-induced citotoxicity in malignantmesothelioma cells (2008)
  • Author(s): Hiromi Sato, Tomohiro Yano, Yasuyuki Takano, Hiroki Iwata, Koichi Ueno
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-29
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