Establishment of a mouse lung-specific gene suppression system using adeno-associated virus carrying short hairpin RNA

• Project/Area Number: 20890052
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Respiratory organ internal medicine
• Research Institution: The University of Tokyo
• Principal Investigator: KAGE Hidenori The University of Tokyo, 医学部附属病院, 助教 (80513390)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: アデノ随伴ウイルス / short hairpin RNA / RNA干渉 / びまん性肺疾患 / Tgfβ1

Research Abstract

We demonstrate that the AAV type 2/5 hybrid vector can carry short hairpin RNA, transduce mouse alveolar epithelium-derived LA-4 cells, and suppress Tgfβ1 expression. This study opens the possibility to suppress an arbitrary gene function in lung alveolar epithelium-derived cells, and help analyze the pathogenesis of pulmonary diseases.

Research Products

• [Presentation] Delivery of short interfering RNA using adeno-associated virus type 2/5 (2010)
  • Author(s): Hidenori Kage
  • Organizer: American Thoracic Society International Conference 2010
  • Place of Presentation: New Orleans USA
  • Year and Date: 2010-05-17
• [Presentation] Delivery of short interfering RNA to alveolar epithelium-derived cells using adeno-associated virus type 2/5 (2010)
  • Author(s): Kage H, Sunohara M, Kawakami M, Kitano K, Emote N, Watanabe K, Aki N, Kusakabe M, Hamano E, Ohishi N, Nagase T, Takai D
  • Organizer: AACR-IASLC Joint Conference on Molecular Origins of Lung Cancer
  • Place of Presentation: Coronado, CA
• [Presentation] Delivery of short interfering RNA using adenoassociated virus type 2/5 (2010)
  • Author(s): Kage H, Sunohara M, Kawakami M, Emoto N, Watanabe K, Aki N, Hamano E, Ohishi N, Nagase T, Takai D
  • Organizer: American Thoracic Society International Conference
  • Place of Presentation: New Orleans, CA
• [Presentation] Knockdown of Tgfb1 in alveolar epithelium-derived LA-4 cells using replication-defective adeno-associated virus carrying short hairpin RNA (2009)
  • Author(s): Hidenori Kage
  • Organizer: American Thoracic Society International Conference 2009
  • Place of Presentation: San Diego
  • Year and Date: 2009-05-19
• [Presentation] Knockdown of Tgfβ1 in alveolar epithelium-derived LA-4 cells using replication-defective adeno-associated virus carrying short hairpin RNA (2009)
  • Author(s): Kage H, Aki N, Emote N, Watanabe K, Kutomi T, Kusakabe M, Hamano E, Kitagawa H, Ohishi N, Nagase T, Yatomi Y, Takai D
  • Organizer: American Thoracic Society International Conference
  • Place of Presentation: San Diego, CA
• [Presentation] Knockdown of Tgfβ1 in alveolar epithelium-derived LA-4 cells using replication-defective adeno-associated virus carrying short hairpin RNA
  • Author(s): Kage H, Aki N, Emote N, Watanabe K, Kutomi T, Kusakabe M, Hamano E, Kitagawa H, Ohishi N, Nagase T, Yatomi Y, Takai D
  • Organizer: 第49回日本呼吸器学会
  • Place of Presentation: 東京