| Budget Amount *help |
¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
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| Research Abstract |
In humans, all GluR2 mRNAs in neurons are completely edited at the Q/R site and the majority of AMPA receptors have GluR2 in their composition, making AMPA receptors Ca^<2+>-impermeable. RNA editing at this site is specifically catalyzed by adenosine deaminase acting on RNA 2 (ADAR2) in mammals including humans. We analyzed the expression levels of ADAR2 mRNA and its activity at the GluR2 Q/R site on laser-captured motor neurons from 29 cases with sporadic ALS compared with those from normal subjects. We demonstrate that inefficient GluR2 Q/R site-editing occurs in a significant proportion of motor neurons in all the ALS cases examined. Universal reduction of ADAR2 in motor neurons is likely a pathognomonic molecular change in sporadic ALS.
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