| Budget Amount *help |
¥3,081,000 (Direct Cost: ¥2,370,000、Indirect Cost: ¥711,000)
Fiscal Year 2009: ¥1,456,000 (Direct Cost: ¥1,120,000、Indirect Cost: ¥336,000)
Fiscal Year 2008: ¥1,625,000 (Direct Cost: ¥1,250,000、Indirect Cost: ¥375,000)
|
|---|
| Research Abstract |
In search of biological activity of 2-benzazepine derivatives, we found that one of 2-benzazepine derivatives (compound A) promoted cell migration of human keratinocyte-derived cell line (HaCat cells) and wound healing of the skin in mouse. Here a structure-function relationship of 2-benzazepine derivatives was evaluated using in vitro wound healing assay (scratch assay) with HaCaT cells. A side chain at N-2 position was important for this biological activity of 2-benzazepine derivatives. The steric size between methyl and ethyl seems optimal at this side chain position, and longer alkyl chain than propyl group at N-2 position spoiled the biological activity. In the scratch assay, compound A -induced migration was blocked by the neutralizing antibody against transforming growth factor-beta (TGF-β), while neutralizing antibody against EGF had no effect. Those results indicate that compound A promotes the epithelial migration increasing the release of TGF-β.
|
