A study on oral function of hyposalivation models

• Project/Area Number: 20890203
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Prosthetic dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: NAKAMOTO Tetsuji Kyushu Dental College, 歯学部, 助教 (30514989)
• Research Collaborator: KONDO Yusuke 九州歯科大学, 歯学部・大学院 ; KIDOKORO Manami 九州歯科大学, 歯学部・大学院 ; MUKAIBOU Taro 九州歯科大学, 歯学部・大学院
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: 唾液腺 / 口腔乾燥症 / イオン / ムスカリン性レセプター / ピロカルピン / セビメリン / 水分泌

Research Abstract

Fluid secretion in response to muscarinic agonists was analyzed using a mouse ex vivo submandibular perfusion technique. Lowering the temperature from the body temperature induced less salivation. Also, increasing the osmolarity in perfusion solution caused less fluid secretion. Pharmacological inhibition on channels, receptors and transporters crucial for fluid secretion also inhibited fluid secretion. Ion concentrations in secreted ex vivo saliva turned out their ion compositions were different among hyposalivaiton models. The results suggest that ion concentration analysis might work for identifying the target which causes hyposalivation.

Research Products

• [Journal Article] 分泌機能を制御するCl-チャネル: Transmembrane Protein 16A/ Anoctamin 1 (2009)
  • Author(s): 中本哲自
  • Journal Title: 九州歯科学会雑誌 63 Pages: 133-137
  • NAID: 130004502045
  • Peer Reviewed
• [Journal Article] 分泌機能を制御するCl-チャネル:Transmembrane Protein 16A/A Anoctamin 1 (2009)
  • Author(s): 中本哲自
  • Journal Title: 九州歯科学会雑誌 63 Pages: 133-137
  • NAID: 130004502045
• [Presentation] 低出力パルス超音波が唾液腺分泌に及ぼす影響 (2009)
  • Author(s): 城所愛美, 近藤祐介, 向坊太郎, 中本哲自, 細川隆司
  • Organizer: 第39回 (社)日本口腔インプラント学会・学術大会,第29回 (社)日本口腔インプラント学会中国・四国支部総会・学術大会併催
  • Place of Presentation: 大阪
  • Year and Date: 2009-09-26
• [Presentation] The relationship between fluid secretion and temperature (2009)
  • Author(s): Mukaibou, T., Nakamoto, T., Kondo, Y., Kidokoro, M., Masaki, C., Hosokawa, R.
  • Organizer: 第57回JADR学術大会2nd Meeting of IADR Pan Asian Pacific Federation (PAPF) 1st Meeting of the IADR Asia/Pacific Region (APR)
  • Place of Presentation: 武漢,中華人民共和国
  • Year and Date: 2009-09-23
• [Presentation] Purinergic P2X7 receptors mediate ATP-induced saliva secretion by the mouse submandibular gland (2009)
  • Author(s): Nakamoto, T., Brown D.A., Catalan, M.A., Gonzalez-Begne, M, Romanenko, V.G., Melvin, J.E.
  • Organizer: 第11回 外分泌腺国際シンポジウム,徳島09
  • Place of Presentation: 徳島
  • Year and Date: 2009-07-24
• [Presentation] Cevimeline and Pilocarpine induced salivation from perfused mouse submandibular gland (2009)
  • Author(s): Kondo, Y., Nakamoto, T., Kidokoro, M., Mukaibou, T., Masaki, C., Hosokawa, R.
  • Organizer: 第11回 外分泌腺国際シンポジウム,徳島09
  • Place of Presentation: 徳島
  • Year and Date: 2009-07-23
• [Presentation] Fluid secretion and osmotic effect in mouse submandibular gland (2009)
  • Author(s): Kidokoro, M., Nakamoto, T., Kondo, Y., Mukaibou, T., Masaki, C., Hosokawa, R.
  • Organizer: 第11回 外分泌腺国際シンポジウム,徳島09
  • Place of Presentation: 徳島
  • Year and Date: 2009-07-23
• [Presentation] The relationship between fluid secretion and temperature (2009)
  • Author(s): Mukaibou, T., Nakamoto, T., Kondo, Y., Kidokoro, M., Masaki, C., Hosokawa, R.
  • Organizer: 第11回 外分泌腺国際シンポジウム,徳島09
  • Place of Presentation: 徳島
  • Year and Date: 2009-07-23
• [Presentation] Cevimeline induced salivation from perfused mouse submandibular glands (2009)
  • Author(s): Kondo, Y., Nakamoto, T., Kidokoro, M., Mukaibou, T., Masaki. C., Hosokawa, R.
  • Organizer: 87th General Session & Exhibition of the IADR
  • Place of Presentation: Miami, USA
  • Year and Date: 2009-04-03
• [Presentation] Cevimeline induced salivation from perfused mouse submandibular glands (2009)
  • Author(s): Kondo, Y., Nakamoto, T., Kidokoro, M., Mukaibou, T., Masaki, C., Hosokawa, R.
  • Organizer: 87th General Session & Exhibition of the IADR
  • Place of Presentation: Miami, FL, USA
  • Year and Date: 2009-04-02
• [Presentation] 低出力パルス超音波が唾液腺分泌に及ぼす影響 (2009)
  • Author(s): 城所愛美, 近藤祐介, 向坊太郎, 正木千尋, 中本哲自, 細川隆司
  • Organizer: 第6回歯科物理刺激研究会
  • Place of Presentation: 北九州
  • Year and Date: 2009-03-28
• [Presentation] Purinergic P2X7 receptors mediate ATP-induced saliva secretion by the mouse submandibular gland (2009)
  • Author(s): Nakamoto, T., Brown, D.A., Catalan, M.A., Gonzalez-Begne, M., Romanenko, V.G., Melvin, J.E.
  • Organizer: 第11回外分泌腺国際シンポジウム, 徳島09
  • Place of Presentation: Tokushima
• [Presentation] Cevimeline and Pilocarpine induced salivation from perfused mouse submandibular gland (2009)
  • Author(s): Kondo, Y. Nakamoto, T. Kidokoro, M. Mukaibo, T. Masaki, C. Hosokawa, R
  • Organizer: 第11回外分泌腺国際シンポジウム, 徳島09
  • Place of Presentation: Tokushima
• [Presentation] Fluid secretion and osmotic effect in mouse submandibular gland (2009)
  • Author(s): Kidokoro, M. Nakamoto, T. Kondo, Y. Mukaibo, T. Masaki, C. Hosokawa, R.
  • Organizer: 第11回外分泌腺国際シンポジウム, 徳島09
  • Place of Presentation: Tokushima
• [Presentation] The relationship between fluid secretion and temperature. (2009)
  • Author(s): Mukaibo, T. Nakamoto, T. Kondo, Y. Kidokoro, M. Masaki, C. Hosokawa, R.
  • Organizer: 第11回外分泌腺国際シンポジウム, 徳島09
  • Place of Presentation: Tokushima
• [Presentation] The relationship between fluid secretion and temperature. (2009)
  • Author(s): Mukaibo, T. Nakamoto, T. Kondo, Y. Kidokoro, M. Masaki, C. Hosokawa, R.
  • Organizer: 第57回JADR学術大会, 2nd Meeting of IADR Pan Asian Pacific Federation(PAPF), 1st Meeting of the IADR Asia/Pacific Region (APR)
  • Place of Presentation: 武漢中国
• [Presentation] 低出力パルス超音波が唾液腺分泌に及ぼす影響, 第39回(社)日本口腔インプラント学会・学術大会 (2009)
  • Author(s): 城所愛美, 近藤祐介, 向坊太郎, 中本哲自, 細川隆司
  • Organizer: 第29回(社)日本口腔インプラント学会中国・四国支部総会・学術大会併催
  • Place of Presentation: 大阪
• [Presentation] マウス顎下腺陰イオン分泌におけるHCO3-の役割-Nkcc1-/-における唾液分泌の検証- (2008)
  • Author(s): 中本哲自, 近藤祐介, Jennifer Scantlin, Mark Wagner, James E. Melvin, 細川隆司
  • Organizer: 第68回九州歯科学会総会
  • Place of Presentation: 北九州
  • Year and Date: 2008-05-31
• [Presentation] マウス灌流顎下腺の温度依存性分泌増強効果 (2008)
  • Author(s): 中本哲自, Jennifer Scantlin, Mark Wagner, James E. Melvin, 細川隆司
  • Organizer: 第68回九州歯科学会総会
  • Place of Presentation: 北九州
  • Year and Date: 2008-05-31
• [Presentation] マウス顎下腺唾液イオン解析による機能評価 (2008)
  • Author(s): 中本哲自, 近藤祐介, 城所愛美, 向坊太郎, 正木千尋, 細川隆司
  • Organizer: 平成20年度(社)日本補綴歯科学会九州・中国・四国支部合同学術大会
  • Place of Presentation: 別府
• [Presentation] Agonist induced Fluid Secretion from Mouse Submandibular gland is Temperature Dependent (2008)
  • Author(s): Nakamoto, T., Hosokwa, R., Melvin J.E.
  • Organizer: 日中歯科医学会大会2008
  • Place of Presentation: Xian CHINA