Protein-expression profiles to develop prognostic biomarkers and molecular function of bone and soft tissue tumors

• Project/Area Number: 20890218
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Juntendo University
• Principal Investigator: SUEHARA Yoshiyuki Juntendo University, 医学部, 助教 (70509405)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: プロテオミクス / 骨軟部腫瘍 / 腫瘍マーカー / GIST / Pfetic / 肉腫 / リン酸化 / 免疫沈降 / 滑膜肉腫 / Pfetin

Research Abstract

For developing prognosis biomarkers of bone and soft tissue tumors, we conducted global protein expression studies using 2D-DIGE. We identified pfetin, which is prognosis biomarker of GISTs and S-protein which is prognosis biomarker of syonovial sarcomas. In order to analyzing the function of pfetin, we examined a immunoprecipitation using pfetin antibody, We identified co-pfetin proteins as candidate protein of prognosis markers for GISTs.

Research Products

• [Journal Article] Pfetin as a Prognostic Biomarker in Gastrointestinal Stromal Tumor: Novel Monoclonal Antibody and External Validation Study in Multiple Clinical Facilities. (2010)
  • Author(s): Kazutaka Kikuta, Yoshiyuki Suehara, Akira Kawai, Setsuo Hirohashi, Tadashi Kondo, et al
  • Journal Title: pn J Clin Oncol 40(inpress) Pages: 60-72
  • Peer Reviewed
• [Journal Article] Late recurrence of giant cell tumor of bone after adjuvant treatment. (2010)
  • Author(s): Yoshiyuki Suehara, Masahiko Nozawa, Sung-gon Kim, Masataka Nagayama, Tomoko Kojima, Tomoaki Torigoe, Daisuke Kubota, Yasuo Yazawa, Toshiharu Matsumoto
  • Journal Title: Journal of Orthopaedic Surgery (in press)
  • Peer Reviewed
• [Journal Article] Treatment outcome of bone and soft tissue sarcoma in elderly patients. (2010)
  • Author(s): Tomoaki Torigoe, Atsuhiko Terakado, Yoshiyuki Suehara, Hisashi Kurosawa
  • Journal Title: Journal of Orthopaedic Surgery (in press)
  • Peer Reviewed
• [Journal Article] GST-P1 as a histological biomarker of synovial sarcoma revealed by proteomics (2009)
  • Author(s): Yoshiyuki Suehara, Kazutaka Kikuta, Robert Nakayama, Naobumi Tochigi, Kunihiko Seki, Hitoshi Ichikawa, Kiyonaga Fujii, Tadashi Hasegawa, Tadakazu Shimoda, Hisashi Kurosawa, Hirokazu Chuman, Yasuo Beppu, Akira Kawai, Setsuo Hirohashi, Tadashi Kondo
  • Journal Title: Proteomics Clin Appl 3(5) Pages: 623-634
  • Peer Reviewed
• [Journal Article] Anatomic site-specific proteomic signatures of gastrointestinal stromal tumors (2009)
  • Author(s): Yoshiyuki Suehara, Kazutaka Kikuta, Robert Nakayama, Kiyonaga Fujii, Hitoshi Ichikawa, Tatsuhiro Shibata Kunihiko Seki, Tadashi Hasegawa, Masahiro Gotoh, Naobumi Tochigi, Tadakazu Shimoda, Yasuhiro Shimada, Takeshi Sano, Yasuo Beppu, Hisashi Kurosawa, SetYoshiyuki Suehara, Kazutaka Kikuta, Rober
  • Journal Title: Proteomics Clin Appl 3(5) Pages: 584-596
  • Peer Reviewed
• [Journal Article] GST-P1 as a histological biomarker of synovial sarcoma revealed by proteomics (2009)
  • Author(s): Yoshiyuki Suehara(1番目15名)
  • Journal Title: Proteomics Clinical Applications 3(5) Pages: 623-634
  • Peer Reviewed
• [Journal Article] Anatomic site-specific proteomic signatures of gastrointestinal stromal tumors (2009)
  • Author(s): Yoshiyuki Suehara(1番目18名)
  • Journal Title: Proteomics Clinical Applications 3(5) Pages: 584-596
  • Peer Reviewed
• [Journal Article] Pfetin, as a prognostic biomarker of gastrointestinal stromal tumors revealed by proteomics (2008)
  • Author(s): Yoshiyuki Suehara, Tadashi Kondo, Kunihiko Seki, Tatsuhiro Shibata, Kiyonaga Fujii, Masahiro Gotoh, Tadashi Hasegawa, Yasuhiro Shimada, Mitsuru Sasako, Tadakazu Shimoda, Hisashi Kurosawa, Yasuo Beppu, Akira Kawai, Setsuo Hirohashi
  • Journal Title: Clin Cancer Res 14 Pages: 1707-1717
  • Peer Reviewed
• [Journal Article] Pfetin predicts outcome for patients with gastrointestinal stromal tumors (2008)
  • Author(s): Yoshiyuki Suehara, et al.
  • Journal Title: Nature Clinical Practice Oncology 5(7) Pages: 364-365
  • Peer Reviewed
• [Journal Article] Protein-expression profiles associated with clinicopathological features of bone and soft tissue sarcomas (2008)
  • Author(s): Akira Kawai, Tadashi Kondo, Yoshiyuki Suehara, Kazutaka Kikuta, Setsuo Hirohashi
  • Journal Title: Clin Orthop Relat Res. 466(9) Pages: 2099-106
  • Peer Reviewed
• [Journal Article] Xanthoma of bone associated with lipoprotein lipase deficiency. (2008)
  • Author(s): Torigoe T, Terakado A, Suehara Y, Kurosawa H.
  • Journal Title: Skeletal Radiol. 37(12) Pages: 1153-6
  • Peer Reviewed
• [Journal Article] Myxoinflammatory fibroblastic sarcoma. (2008)
  • Author(s): Kobayashi E, Kawai A, Endo M, Suehara Y, Takeda K, Nakatani F, Asano T, Sakuraba M, Chuman H, Seki K, Beppu Y.
  • Journal Title: J Orthop Sci. 13(6) Pages: 566-71
  • NAID: 10025353332
  • Peer Reviewed
• [Journal Article] 骨軟部肉腫の網羅的発現解析 (2008)
  • Author(s): 末原義之
  • Journal Title: 癌と化学療法:Current Organ Topics 35(3) Pages: 407-410
• [Journal Article] 悪性骨・軟部腫瘍(滑膜肉腫)におけるプロテオーム解析および新たな腫瘍マーカー開発に関する研究 (2008)
  • Author(s): 末原義之
  • Journal Title: 整形外科療法:整形外科トピックス Vol.59No.6 Pages: 406-406
• [Journal Article] Pfetin as a prognostic biomarker of gastrointestinal stromal tumors revealed by proteomics (2008)
  • Author(s): Suehara Y, et al.
  • Journal Title: Clinical Cancer Research 15 ; 14(6) Pages: 1707-1707
  • Peer Reviewed
• [Journal Article] Pfetin predicts outcome for patients with gastrointestinal stromal tumors (2008)
  • Author(s): Suehara Y, et al.
  • Journal Title: Nature Clinical Practice Oncology 5(7) Pages: 364-365
• [Journal Article] Protein-expression profiles associated with clinicopathological features of bone and soft tissue sarcomas (2008)
  • Author(s): Kawai A, et al.
  • Journal Title: Clinical Orthopedics Related Research 466(9) Pages: 2099-2106
  • Peer Reviewed
• [Presentation] 滑膜肉腫組織亜型に関するプロテオーム解析(Proteomics signatures corresponding to synovial sarcoma subtypes) (2009)
  • Author(s): 末原義之
  • Organizer: 第24回日本整形外科学基礎学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2009-11-06
• [Presentation] 腫瘍マーカーの開発を目的とした骨軟部腫瘍におけるプロテオーム解析 (2009)
  • Author(s): 末原義之
  • Organizer: 中央区港区整形外科医会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-23
• [Presentation] GST-P1 as a histological biomarker of synovial sarcoma revealed by proteomics(滑膜肉腫組織亜型に関するプロテオーム解析) (2009)
  • Author(s): 末原義之
  • Organizer: 日本癌学会68回総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-03
• [Presentation] GST-p1 as a histological biomarker of synovial sarcoma revealed by proteomics (2009)
  • Author(s): 末原義之
  • Organizer: 15^<th>, International Symposium on Limb Salvage
  • Place of Presentation: Boston, USA
  • Year and Date: 2009-09-24
• [Presentation] 消化管間質腫瘍(GIST)の予後予測マーカーの開発を目的としたプロテオミクスの検討 (2009)
  • Author(s): 末原義之
  • Organizer: 第95回日本消化器病学会:シンポジウム:GIST研究の進歩と臨床応用
  • Place of Presentation: 札幌
  • Year and Date: 2009-05-07
• [Presentation] GST- p1 as a histological biomarker of synovial sarcoma revealed by proteomics. (2009)
  • Author(s): Yoshiyuki Suehara, Akira Kawai, Tadashi Kondo, et al
  • Organizer: 15th, International Symposium on Limb Salvage
  • Place of Presentation: Boston, USA
• [Presentation] 滑膜肉腫組織亜型に関するプロテオーム解析(Proteomics signatures corresponding to synovial sarcoma subtypes) (2009)
  • Author(s): 末原義之菊田一貴栃木直文中馬広一川井章別府保男廣橋説雄近藤格
  • Organizer: 第6回関東骨軟部基礎を語る会
  • Place of Presentation: 東京
• [Presentation] ワークショップ:GIST研究の進歩と臨床応用:消化管間質腫瘍(GIST)の予後予測マーカーの開発を目的としたプロテオミクスの検討 (2009)
  • Author(s): 末原義之渡辺純夫近藤格
  • Organizer: 第95回日本消化器病学会
  • Place of Presentation: 札幌
• [Presentation] 滑膜肉腫組織亜型に関するプロテオーム解析(Proteomics signatures corresponding to synovial sarcoma subtypes) (2009)
  • Author(s): 末原義之菊田一貴栃木直文中馬広一川井章別府保男廣橋説雄近藤格
  • Organizer: 第82回日本整形外科学会学術総会
  • Place of Presentation: 福岡
• [Presentation] GST-P1 as a histological biomarker of synovial sarcoma revealed by proteomics(滑膜肉腫組織亜型に関するプロテオーム解析) (2009)
  • Author(s): 末原義之菊田一貴栃木直文中馬広一川井章別府保男廣橋説雄近藤格
  • Organizer: 日本癌学会68回総会
  • Place of Presentation: 東京
• [Presentation] 招待講演:腫瘍マーカーの開発を目的とした骨軟部腫瘍におけるプロテオーム解析 (2009)
  • Author(s): 末原義之
  • Organizer: 中央区港区整形外科医会学術講演会
  • Place of Presentation: 東京
• [Presentation] 滑膜肉腫組織亜型に関するプロテオーム解析(Proteomics signatures corresponding to synovial sarcoma subtypes) (2009)
  • Author(s): 末原義之菊田一貴栃木直文中馬広一川井章別府保男廣橋説雄近藤格
  • Organizer: 第24回日本整形外科学基礎学術集会
  • Place of Presentation: 東京
• [Presentation] Grobal protein-expression analysis of bone and soft tissue tumors. (2008)
  • Author(s): Yoshiyuki Suehara, Akira Kawai, Setsuo Hirohashi, Tadashi Kondo, et al
  • Organizer: 13eme Reunion de Societe Franco-Japonaise d'Orthopedie.
  • Place of Presentation: Tokyo
• [Presentation] Pfetin, as a prognostic biomarker of gastrointestinal stromal tumors revealed by proteomics. (2008)
  • Author(s): Yoshiyuki Suehara, Kunihiko Seki, Kiyonaga Fujii, Tadashi Hasegawa, Tadakazu Shimoda, Yasuo Beppu, Akira Kawai, Setsuo Hirohashi, Tadashi Kondo
  • Organizer: 14th Annual Connective Tissue Oncology Society Meeting
  • Place of Presentation: London, UK: CTOS Young Investigator Award
• [Presentation] 招待講演(教育研修講演)骨軟部腫瘍における新規バイオマーカーの探索-プロテオミクスの視点から- (2008)
  • Author(s): 末原義之
  • Organizer: 第5回関東骨軟部基礎を語る会
  • Place of Presentation: 東京
• [Presentation] 骨肉腫の化学療法感受性予測マーカーの開発を目的としたプロテオミクスの検討 (2008)
  • Author(s): 末原義之近藤格長谷川匡関邦彦下田忠和中馬広一川井章別府保男廣橋説雄
  • Organizer: 第41回日本整形外科学会・骨軟部腫瘍学術集会
  • Place of Presentation: 横浜
• [Presentation] 消化管間質腫瘍(GISTs)の予後予測マーカーの開発を目的としたプロテオミクスの検討 (2008)
  • Author(s): 末原義之近藤格長谷川匡関邦彦下田忠和川井章別府保男廣橋説雄
  • Organizer: 第41回日本整形外科学会・骨軟部腫瘍学術集会
  • Place of Presentation: 横浜
• [Presentation] シンポジウム骨・軟部腫瘍の治療:Future perspectives(骨・軟部腫瘍治療の新展開):骨・軟部腫瘍の予後・組織マーカーの開発を目的としたプロテオーム解析 (2008)
  • Author(s): 末原義之近藤格長谷川匡関邦彦栃木直文下田忠和中馬広一川井章別府保男廣橋説雄
  • Organizer: 第23回日本整形外科学基礎学術集会
  • Place of Presentation: 京都
• [Presentation] Proteomic signature corresponding to original site of gastrointestinal stromal tumor (2008)
  • Author(s): 末原義之菊田一貴中山ロバート藤井清永市川仁長谷川匡関邦彦栃木直文下田忠和川井章別府保男廣橋説雄近藤格
  • Organizer: 日本癌学会67回総会
  • Place of Presentation: 名古屋
• [Presentation] 招待講演:腫瘍マーカーの開発を目的とした骨軟部腫瘍におけるプロテオーム解析 (2008)
  • Author(s): 末原義之
  • Organizer: 第164回骨・軟部腫瘍症例検討会
  • Place of Presentation: 東京
• [Presentation] Pfetin, as a prognostic biomarker of gastrointestinal stromal tumors revealed by proteomics (2008)
  • Author(s): Suehara Y, et al.
  • Organizer: 14th Annual Connective Tissue Oncology Society Meeting
  • Place of Presentation: London, UK
• [Book] がん看護実践シリーズ12;骨軟部腫瘍 (2008)
  • Author(s): 末原義之川井章
• [Book] がん看護 実践シリーズ12 ; 骨軟部腫瘍 (2008)
  • Author(s): 末原義之(共著)
  • Publisher: メヂカルフレンド社
• [Patent(Industrial Property Rights)] セセルニン-1の使用、滑膜肉腫の予後予測方法及び検査用試薬のキット (2009)
  • Inventor(s): 近藤格、川井章、末原義之
  • Industrial Property Rights Holder: 近藤格、川井章、末原義之
  • Industrial Property Number: 2009-285045
  • Filing Date: 2009-12-16
  • Acquisition Date: 2009-12-16
• [Patent(Industrial Property Rights)] セセルニン-1の使用、滑膜肉腫の予後予測方法及び検査用試薬のキット (2009)
  • Inventor(s): 末原義之, 他
  • Industrial Property Rights Holder: 末原義之, 他
  • Industrial Property Number: 2009-285045
  • Filing Date: 2009-12-16