Functional characterization of Porphyromonas gingivalis Mfa1 fimbriae

• Project/Area Number: 20890248
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: HASEGAWA Yoshiaki Aichi Gakuin University, 歯学部・微生物学講座, 講師 (70460524)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥2,743,000 (Direct Cost: ¥2,110,000、Indirect Cost: ¥633,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,703,000 (Direct Cost: ¥1,310,000、Indirect Cost: ¥393,000)
• Keywords: 細菌 / 歯周病 / Porphyromonas gingivalis / Mfa1線毛 / 微量成分 / P.gingivalis / PGN0288 / Mfa2 / 短線毛 / P. gingivalis

Research Abstract

In this study we focused on the four ORFs (mfa2, PG0180, PG0181, and PG0182) downstream of the mfa1 gene encoding the major fimbrilin Mfa1. We demonstrated that mfa2 is involved in the length regulation of the Mfa1 fimbriae. Neither PG0180 nor PG0181 were present in the purified Mfa1 fimbriae from the PG0182 mutant, suggesting that PG0182 is required for the assembly of PG0180 and PG0181 into fiber. The mutants of downstream genes increased autoaggregation activity compared with the parent strain. These data indicates that downstream products of the mfa1 gene are involved in function and structure of Mfa1 fimbriae.

Research Products

• [Journal Article] (2009)
  • Author(s): Yoshiaki Hasegawa, Jun Iwami, Keiko Sato, Yoonsuk Park, Tatsuo Atsumi, Keiichi Moriguchi, Yukitaka Murakami, Richard J. Lamont, Hiroshi Nakamura, Norikazu, Ohno, Fuminobu Yoshimura
  • Journal Title: Anchoring and length regulation of Porphyromonas gingivalis Mfa1 fimbriae by the down stream gene product Mfa2 vol.155 Pages: 3333-3347
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalisのMfa1線毛の微量成分遺伝子mfa2の機能解析 (2009)
  • Author(s): 長谷川義明, 尾関万里奈, 西川清, 村上幸孝, 永野恵司, 吉村文信
  • Journal Title: Bacterial Adherence and Biofilm 22巻 Pages: 95-101
• [Journal Article] Anchoring and length regulation of Porphyromonas gingivalis Mfa1 fimbriae by the downstream gene product Mfa2 (2009)
  • Author(s): Hasegawa, Y., Iwami, J., Sato, K., Park, Y., Nishikawa, K., Atsumi, T., Moriguchi, K., Murakami, Y., Lamont, R.J., Nakamura, H., Ohno, N., Yoshimura, F.
  • Journal Title: Microbiology VOL.155 Pages: 3333-3347
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalis のMfa1線毛の微量成分遺伝子mfa2の機能解析 (2009)
  • Author(s): 長谷川義明, 尾関万里奈, 西川清, 村上幸孝, 永野恵司, 吉村文信
  • Journal Title: Bacterial Adherence and Biofilm 22巻 Pages: 95-101
• [Presentation] Localization of Mfa2, a length regulator of Porphyromonas gingivalis Mfa1 fimbriae (2010)
  • Author(s): 長谷川義明, 永野恵司, 村上幸孝, 吉村文信
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2010-03-27
• [Presentation] Roles of FimB for FimA fimbriae expression in Porphyromonas gingivalis (2010)
  • Author(s): 永野恵司, 長谷川義明, 村上幸孝, 吉村文信
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2010-03-27
• [Presentation] 歯周病関連細菌Porphyromonas gingivalisのMfa1線毛の長さの制御を担うMfa2の局在と機能に関する研究 (2010)
  • Author(s): 吉村文信, 長谷川義明, 永野恵司, 村上幸孝
  • Organizer: 第39回東海乳酸菌研究会
  • Place of Presentation: ビル(名古屋)
  • Year and Date: 2010-02-06
• [Presentation] 歯周病関連細菌 Porphyromonas gingivalis のMfa1線毛の長さの制御を担うMfa2の局在と機能に関する研究 (2010)
  • Author(s): 吉村文信, 長谷川義明, 永野恵司, 村上幸孝
  • Organizer: 第39回東海乳酸菌研究会
  • Place of Presentation: 中日ビル(名古屋)
  • Year and Date: 2010-02-06
• [Presentation] 歯周病関連細菌Porphyromonas gingivalisのFimA線毛の形態および機能に及ぼすFimBの役割に関する研究 (2009)
  • Author(s): 永野恵司, 長谷川義明, 村上幸孝, 吉村文信
  • Organizer: 第46回日本細菌学会中部支部総会
  • Place of Presentation: 名城大学薬学部(名古屋)
  • Year and Date: 2009-10-24
• [Presentation] 歯周病関連細菌 Porphyromonas gingivalis のFimA線毛の形態および機能に及ぼすFimBの役割に関する研究 (2009)
  • Author(s): 永野恵司, 長谷川義明, 村上幸孝, 吉村文信
  • Organizer: 第46回日本細菌学会中部支部総会
  • Place of Presentation: 名城大学薬学部(名古屋)
  • Year and Date: 2009-10-24
• [Presentation] Porphyromonas gingivalisのFimA線毛の形態および機能に及ぼすFimBの役割に関する研究 (2009)
  • Author(s): 永野恵司, 長谷川義明, 村上幸孝, 西山宗一郎, 吉村文信
  • Organizer: 第74回愛知学院大学歯学会学術大会
  • Place of Presentation: (名古屋)
  • Year and Date: 2009-06-14
• [Presentation] Role of mfa2 in Porphyromonas gingivalis Mfa1 fimbriae. (2009)
  • Author(s): Yoshiaki Hasegawa, Jun Iwami, Keiichi Moriguchi, Yukitaka Murakami, Norikazu Ohno, Hiroshi Nakamura, Richard J. Lamont, Fuminobu Yoshimura
  • Organizer: 87th General session and Exhibition of the IADR
  • Place of Presentation: Miami Beach Convention Center (Miami)
  • Year and Date: 2009-04-03
• [Presentation] Role of mfa2 in Porphyromonas gingivalis Mfa1 fimbriae (2009)
  • Author(s): Hasegawa, Y., Iwami, J., Moriguchi, K., Murakami, Y., Ohno, N., Nakamura, H., Lamont, R.J., Yoshimura, F.
  • Organizer: 87^<th> General session and Exhibition of the IADR
  • Place of Presentation: Miami Beach Convention Center(Miami)
  • Year and Date: 2009-04-03
• [Presentation] Porphyromonas gingivalisのMfa1線毛微量成分遺伝子の解析と変異株の作製 (2008)
  • Author(s): 長谷川義明, 尾関万里奈, 村上幸孝, 吉村文信
  • Organizer: 第50回歯科基礎医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-25
• [Presentation] Porphyromonas gingivalisのMfa1線毛微量成分遺伝子の解析と変異株作製 (2008)
  • Author(s): 長谷川義明
  • Organizer: 第50回歯科基礎医学会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-25
• [Presentation] Porphyromonas gingivalisのMfa1線毛の微量成分遺伝子mfa2の機能解析 (2008)
  • Author(s): 長谷川義明, 尾関万里奈, 西川清, 村上幸孝, 永野恵司, 吉村文信
  • Organizer: Bacterial Adherence and Biofilm
  • Place of Presentation: 兵庫県立淡路夢舞台国際会議場(兵庫)
  • Year and Date: 2008-07-04
• [Presentation] Porphyromonas gingivalisのMfa1線毛の微量成分遺伝子mfa2の機能解析 (2008)
  • Author(s): 長谷川義明
  • Organizer: Bacterial adherence and biofilm 第22回学術集会
  • Place of Presentation: 兵庫
  • Year and Date: 2008-07-04