Functional characterization of Porphyromonas gingivalis Mfa1 fimbriae
| Project/Area Number |
20890248
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
HASEGAWA Yoshiaki Aichi Gakuin University, 歯学部・微生物学講座, 講師 (70460524)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥2,743,000 (Direct Cost: ¥2,110,000、Indirect Cost: ¥633,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,703,000 (Direct Cost: ¥1,310,000、Indirect Cost: ¥393,000)
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| Keywords | 細菌 / 歯周病 / Porphyromonas gingivalis / Mfa1線毛 / 微量成分 / P.gingivalis / PGN0288 / Mfa2 / 短線毛 / P. gingivalis |
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| Research Abstract |
In this study we focused on the four ORFs (mfa2, PG0180, PG0181, and PG0182) downstream of the mfa1 gene encoding the major fimbrilin Mfa1. We demonstrated that mfa2 is involved in the length regulation of the Mfa1 fimbriae. Neither PG0180 nor PG0181 were present in the purified Mfa1 fimbriae from the PG0182 mutant, suggesting that PG0182 is required for the assembly of PG0180 and PG0181 into fiber. The mutants of downstream genes increased autoaggregation activity compared with the parent strain. These data indicates that downstream products of the mfa1 gene are involved in function and structure of Mfa1 fimbriae.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article]2009
Author(s)
Yoshiaki Hasegawa, Jun Iwami, Keiko Sato, Yoonsuk Park, Tatsuo Atsumi, Keiichi Moriguchi, Yukitaka Murakami, Richard J. Lamont, Hiroshi Nakamura, Norikazu, Ohno, Fuminobu Yoshimura
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Journal Title
Anchoring and length regulation of Porphyromonas gingivalis Mfa1 fimbriae by the down stream gene product Mfa2 vol.155
Pages: 3333-3347
Related Report
Peer Reviewed
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[Journal Article] Anchoring and length regulation of Porphyromonas gingivalis Mfa1 fimbriae by the downstream gene product Mfa22009
Author(s)
Hasegawa, Y., Iwami, J., Sato, K., Park, Y., Nishikawa, K., Atsumi, T., Moriguchi, K., Murakami, Y., Lamont, R.J., Nakamura, H., Ohno, N., Yoshimura, F.
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Journal Title
Microbiology VOL.155
Pages: 3333-3347
Related Report
Peer Reviewed
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[Presentation] Role of mfa2 in Porphyromonas gingivalis Mfa1 fimbriae.2009
Author(s)
Yoshiaki Hasegawa, Jun Iwami, Keiichi Moriguchi, Yukitaka Murakami, Norikazu Ohno, Hiroshi Nakamura, Richard J. Lamont, Fuminobu Yoshimura
Organizer
87th General session and Exhibition of the IADR
Place of Presentation
Miami Beach Convention Center (Miami)
Year and Date
2009-04-03
Related Report
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[Presentation] Role of mfa2 in Porphyromonas gingivalis Mfa1 fimbriae2009
Author(s)
Hasegawa, Y., Iwami, J., Moriguchi, K., Murakami, Y., Ohno, N., Nakamura, H., Lamont, R.J., Yoshimura, F.
Organizer
87^<th> General session and Exhibition of the IADR
Place of Presentation
Miami Beach Convention Center(Miami)
Year and Date
2009-04-03
Related Report
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