HMBA-induced erythroid differentiation decreases sphingosine kinase 1 gene expression of a mouse erythroleukemia cell line

• Project/Area Number: 20890251
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Chubu University
• Principal Investigator: SOBUE Sayaka Chubu University, 生命健康科学部, 助手 (50513347)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: SPHK1 / 赤白血病 / 分子標的療法 / sphingosine kinase 1 / 白血病 / 分化誘導療法

Research Abstract

Friend cell is a mouse erythroleukemia cell line inducible to the erythroid lineage with HMBA. Increased expression of sphingosine kinase 1 (SPHK1), a sphingolipid metabolic enzyme, has been reported in Friend cells. In the current study, we analyzed the mechanism of its SPHK1 overexpression and the sequential changes during HMBA-induced differentiation.

Research Products

• [Journal Article] ATRA inhibits ceramide kinase transcription in a human neuroblastoma cell line, SH-SY5Y cells : the role of COUP-TFI. (2010)
  • Author(s): Murakami M, Ito H, Hagiwara K, Yoshida K, Sobue S, Ichihara M, Takagi A, Kojima T, Tanaka K, Koizumi-T K, Kyogashima M, Suzuki M, Banno Y, Nozawa Y, Murate T.
  • Journal Title: J Neurochem. 112 Pages: 511-520
  • Peer Reviewed
• [Journal Article] ATRA inhibits ceramide kinase transcription in a human neuroblastoma cell line, SH-SY5Y cells : the role of COUP-TFI (2010)
  • Author(s): Murakami M.
  • Journal Title: J Neurochem. 112 Pages: 511-520
  • Peer Reviewed
• [Journal Article] GATA-1 and GATA-2 binding to 3' enhancer of WT1 gene is essential for its transcription in acute leukemia and solid tumor cell lines. (2009)
  • Author(s): Furuhata A, Murakami M, Ito H, Gao S, Yoshida K, Sobue S, Kikuchi R, Iwasaki T, Takagi A, Kojima T, Suzuki M, Abe A, Naoe T, Murate T.
  • Journal Title: Leukemia. 23 Pages: 1270-1277
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of neutral sphingomyelinase 2 gene expression of a human breast cancer cell line, MCF-7, induced by the anti-cancer drug, daunorubicin. (2009)
  • Author(s): Ito H, Murakami M, Furuhata A, Gao S, Yoshida K, Sobue S, Hagiwara K, Takagi A, Kojima T, Suzuki M, Banno Y, Tanaka K, Tamiya-Koizumi K, Kyogashima M, Nozawa Y, Murate T.
  • Journal Title: Biochim Biophys Acta. 1789 Pages: 681-690
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of neutral sphingomyelinase 2 gene expression of a human breast cancer cell line, MCF-7, induced by the anti-cancer drug, daunorubicin (2009)
  • Author(s): Ito H.
  • Journal Title: Biochim Biophys Acta. 1789 Pages: 681-690
  • Peer Reviewed
• [Journal Article] GATA-1 and GATA-2 binding to 3' enhancer of WT1 gene is essential for its transcription in acute leukemia and solid tumor cell lines (2009)
  • Author(s): A Furuhata
  • Journal Title: Leukemia (In press)
  • Peer Reviewed
• [Journal Article] Implications of sphingosine kinase 1 expression level for the cellular sphingolipid rheostat : relevance as a marker for daunorubicin sensitivity of leukemia cells. (2008)
  • Author(s): Sobue S, Nemoto S, Murakami M, Ito H, Kimura A, Gao S, Furuhata A, Takagi A, Kojima T, Nakamura M, Ito Y, Suzuki M, Banno Y, Nozawa Y, Murate T.
  • Journal Title: Int J Hematol. 87 Pages: 266-275
  • Peer Reviewed
• [Journal Article] Characterization of myelodysplastic syndrome and aplastic anemia by immunostaining of p53 and hemoglobin F and karyotype analysis: differential diagnosis between refractory anemia and aplastic anemia. (2008)
  • Author(s): Iwasaki T, Murakami M, Sugisaki C, Sobue S, Ohashi H, Asano H, Suzuki M, Nakamura S, Ito M, Murate T.
  • Journal Title: Pathol Int. 58 Pages: 353-360
  • NAID: 10027376865
  • Peer Reviewed
• [Journal Article] v-Src oncogene product increases sphingosine kinase 1 expression through mRNA stabilization : alteration of AU-rich element-binding proteins. (2008)
  • Author(s): Sobue S, Murakami M, Banno Y, Ito H, Kimura A, Gao S, Furuhata A, Takagi A, Kojima T, Suzuki M, Nozawa Y, Murate T.
  • Journal Title: Oncogene. 27 Pages: 6023-6033
  • Peer Reviewed
• [Journal Article] Implications of sphingosine kinase 1 expression level for the cellular sphingolipid rheostat : relevance as a marker for daunorubicin sensitivity of leukemia cells. (2008)
  • Author(s): Sobue S
  • Journal Title: Int J Hematol 87 Pages: 266-275
  • Peer Reviewed
• [Journal Article] Characterization of myelodysplastic syndrome and aplastic anemia by immunostaining of p53 and hemoglobin F and karyotype analysis : differential diagnosis between refractory anemia and aplastic anemia (2008)
  • Author(s): Iwasaki T
  • Journal Title: Pathol Int 58 Pages: 353-360
  • Peer Reviewed
• [Journal Article] v-Src oncogene product increases sphingosine kinase 1 expression through mRNA stabilization : alteration of AU-rich element-binding proteins. (2008)
  • Author(s): Sobue S
  • Journal Title: Oncogene 27 Pages: 6023-6033
  • Peer Reviewed
• [Presentation] 水素水投与後の血中水素濃度動態の検討 (2010)
  • Author(s): 山井一晃
  • Organizer: 第2回水素研究会学術集会
  • Place of Presentation: アルカディア市ヶ谷(東京都)
  • Year and Date: 2010-02-12
• [Presentation] ヒト乳癌細胞株MCF7細胞におけるATRAによるNSMase2の発現調節 (2009)
  • Author(s): 伊藤裕美
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2009-10-22
• [Presentation] v-SrcによるSPHK1 mRNA安定化と発現増強 (2008)
  • Author(s): 祖父江沙矢加
  • Organizer: 第81回日本生化学会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2008-12-09
• [Presentation] Regulatory mechanism of neutral sphingomyelinase 2 gene expression by daunorubicin in MCF-7 cells. (2008)
  • Author(s): Hiromi Ito
  • Organizer: 第81回日本生化学会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2008-12-09
• [Presentation] ATRA inhibits ceramide kinase transcription through an ATRA-related transcription factor, COUP-TF1, in a human neuroblastoma cell line, SH-SY5Y cells. (2008)
  • Author(s): Masashi Murakami
  • Organizer: 第81回日本生化学会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2008-12-09
• [Presentation] Mutated Ras-induced phospholipase D1 transcription in a human colon adenocarcinoma cell line, DLD-1. (2008)
  • Author(s): Siqiang Gao
  • Organizer: 第81回日本生化学会
  • Place of Presentation: 神戸ポートアイランド(神戸市)
  • Year and Date: 2008-12-09
• [Presentation] Regulatory mechanism of neutral sphingomyelinase 2 gene expression by daunorubicin in MCF-7 cells (2008)
  • Author(s): 伊藤裕美
  • Organizer: 第81回日本生化学会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Presentation] ATRA inhibits ceramide kinase transcription through an ATRA-related transcription factor, COUP-TF1, in a human neuroblastoma cell line, SH-SY5Y cells. (2008)
  • Author(s): 村上真史
  • Organizer: 第81回日本生化学会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Presentation] v-SrcによるSPHK1 mRNA安定化と発現増強 (2008)
  • Author(s): 祖父江沙矢加
  • Organizer: 第81回日本生化学会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Presentation] Mutated Ras-induced phospholipase D1 transcriotion in a human colon adenocarcinoma cell line, DLD-1. (2008)
  • Author(s): 高四強
  • Organizer: 第81回日本生化学会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)